Overview
- External ontology
- MEDIADB:308 (source link unavailable) — Kaminskyj Minimal Medium
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- High-metal flag
- True
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007206["kaminskyj_minimal_medium"]
ing0_Sodium_nitrate["Sodium nitrate"]
CultureMech_007206 --> ing0_Sodium_nitrate
CHEBI_63005["CHEBI:63005"]
ing0_Sodium_nitrate -.-> CHEBI_63005
ing1_Sodium_EDTA["Sodium EDTA"]
CultureMech_007206 --> ing1_Sodium_EDTA
ing2_D_Glucose["D-Glucose"]
CultureMech_007206 --> ing2_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing2_D_Glucose -.-> CHEBI_17634
ing3_Cobalt_chloride["Cobalt chloride"]
CultureMech_007206 --> ing3_Cobalt_chloride
CHEBI_35696["CHEBI:35696"]
ing3_Cobalt_chloride -.-> CHEBI_35696
ing4_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007206 --> ing4_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing4_Ferrous_sulfate -.-> CHEBI_75832
ing5_Sodium_molybdate["Sodium molybdate"]
CultureMech_007206 --> ing5_Sodium_molybdate
CHEBI_75215["CHEBI:75215"]
ing5_Sodium_molybdate -.-> CHEBI_75215
ing6_Cupric_sulfate["Cupric sulfate"]
CultureMech_007206 --> ing6_Cupric_sulfate
CHEBI_23414["CHEBI:23414"]
ing6_Cupric_sulfate -.-> CHEBI_23414
ing7_Manganese_chloride["Manganese chloride"]
CultureMech_007206 --> ing7_Manganese_chloride
CHEBI_63041["CHEBI:63041"]
ing7_Manganese_chloride -.-> CHEBI_63041
ing8_Boric_acid["Boric acid"]
CultureMech_007206 --> ing8_Boric_acid
CHEBI_33118["CHEBI:33118"]
ing8_Boric_acid -.-> CHEBI_33118
ing9_Thiamine["Thiamine"]
CultureMech_007206 --> ing9_Thiamine
CHEBI_18385["CHEBI:18385"]
ing9_Thiamine -.-> CHEBI_18385
ing10_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007206 --> ing10_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing10_Magnesium_sulfate -.-> CHEBI_32599
ing11_Potassium_chloride["Potassium chloride"]
CultureMech_007206 --> ing11_Potassium_chloride
CHEBI_32588["CHEBI:32588"]
ing11_Potassium_chloride -.-> CHEBI_32588
ing12_Zinc_sulfate["Zinc sulfate"]
CultureMech_007206 --> ing12_Zinc_sulfate
CHEBI_35176["CHEBI:35176"]
ing12_Zinc_sulfate -.-> CHEBI_35176
ing13_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007206 --> ing13_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing13_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Sodium nitrate
NO3.Na 84.994 g/mol
|
1411.9 MILLIMOLAR |
CHEBI:63005
sodium nitrate
|
|
| 2 | Sodium EDTA | 0.1315 MILLIMOLAR | ||
| 3 |
D-Glucose
C6H12O6 180.156 g/mol
|
55.51 MILLIMOLAR |
CHEBI:17634
D-glucose
|
Cross-references: KEGG:glc_D |
| 4 |
Cobalt chloride
2Cl.Co 129.839 g/mol
|
0.007145 MILLIMOLAR |
CHEBI:35696
cobalt dichloride
|
|
| 5 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.01798 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 6 |
Sodium molybdate
MoO4.2Na 205.916 g/mol
|
0.006199 MILLIMOLAR |
CHEBI:75215
sodium molybdate (anhydrous)
|
|
| 7 |
Cupric sulfate
Cu.O4S 159.609 g/mol
|
0.006408 MILLIMOLAR |
CHEBI:23414
copper(II) sulfate
|
|
| 8 |
Manganese chloride
Cl2Mn 125.844 g/mol
|
0.02526 MILLIMOLAR |
CHEBI:63041
manganese(II) chloride
|
|
| 9 |
Boric acid
H3BO3 61.833 g/mol
|
0.1779 MILLIMOLAR |
CHEBI:33118
boric acid
|
|
| 10 |
Thiamine
C12H17N4OS 265.362 g/mol
|
0.03769 MILLIMOLAR |
CHEBI:18385
thiamine(1+)
|
Cross-references: KEGG:thm |
| 11 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
42.2 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 12 |
Potassium chloride
Cl.K 74.551 g/mol
|
139.5 MILLIMOLAR |
CHEBI:32588
potassium chloride
|
|
| 13 |
Zinc sulfate
O4S.Zn 161.453 g/mol
|
0.07651 MILLIMOLAR |
CHEBI:35176
zinc sulfate
|
|
| 14 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
223.4 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- kaminskyj_minimal_medium (CultureMech:007206)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:45.401642Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 308, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-01-28T19:48:30.155979Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:46.867583Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007206 |
| 2026-03-17T06:30:29.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:05.050088+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Kaminskyj Minimal Medium" to "kaminskyj_minimal_medium" for consistent matching |
| 2026-06-05T07:41:10.337033+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:39.529646+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint e53e4997a5a89e956d64fa6231762aa57ddc2c70843e01bceb14abbe4aad634f |