kaminskyj_minimal_medium

Overview

External ontology

MEDIADB:308 (source link unavailable) — Kaminskyj Minimal Medium

Applications

• Cultivation of genome-sequenced organisms
• Metabolic modeling
• Systems biology research

pH

6.5

High-metal flag

True

Notes

Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007206["kaminskyj_minimal_medium"]
    ing0_D_Glucose["D-Glucose"]
    CultureMech_007206 --> ing0_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing0_D_Glucose -.-> CHEBI_17634
    ing1_Distilled_water["Distilled water"]
    CultureMech_007206 --> ing1_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Distilled_water -.-> CHEBI_15377

Ingredients (2)

Preparation

1. Combine 10 g D-glucose, 50 mL 20X nitrate stock, 1 mL trace stock and 1 mL 1% thiamine solution for one litre of MM. Add nutritional supplements only if required by the strain.
2. Adjust to pH 6.5 using 1 M sodium hydroxide. The amount required is not specified.
3. Make up to a final volume of 1 L with distilled water.
4. Autoclave. The source does not specify temperature or duration for this step.

Solutions

Target organisms

No target organisms are recorded.

Recipe variants

• Name

  Anhydrous magnesium sulfate option

  Relationship

  SUBSTITUTED_COMPONENT_VARIANT

  Modifications

  • In the 20X nitrate stock, replace 10.4 g magnesium sulfate heptahydrate per 1 L stock with 5.2 g anhydrous magnesium sulfate per 1 L stock. Keep the stock addition at 50 mL/L final medium.

  Evidence

  • Reference

    https://www.fgsc.net/fgn48/Kaminskyj.htm

    Supports

    SUPPORT

    Snippet

    MgSO 4 ·7H 2 O 10.4 g (5.2 g if anhydrous)

    Explanation

    variants[0]: nitrate-stock magnesium row, source-explicit alternative, not an extra ingredient. Separate volume excerpt: 'Add distilled water to 1 liter.' Thus 5.2 g / 1 L = 5.2 g/L anhydrous salt in stock; 5.2 * 0.050 = 0.26 g/L final medium. Source: doi:10.4148/1941-4765.1175; accessed 2026-10-09.

• Name

  Solid minimal medium

  Relationship

  PHYSICAL_STATE_VARIANT

  Modifications

  • Add 18 g agar to the one-litre MM preparation for solid medium; retain the source's autoclaving step.

  Evidence

  • Reference

    https://www.fgsc.net/fgn48/Kaminskyj.htm

    Supports

    SUPPORT

    Snippet

    For solid medium add 18 g agar.

    Explanation

    variants[1]: MM preparation, not CM (which instead specifies 15 g). Separate excerpt: 'Make to 1 liter with distilled water.' Source states 18 g per one-litre preparation; no separate post-agar final volume is invented. Base remains LIQUID. Source: doi:10.4148/1941-4765.1175; accessed 2026-10-09.

Provenance