Overview
- External ontology
- MEDIADB:35 (source link unavailable) — Minimal medium; whitchurch et al
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- High-metal flag
- True
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007261["minimal_medium_whitchurch_et_al"]
ing0_Magnesium_chloride["Magnesium chloride"]
CultureMech_007261 --> ing0_Magnesium_chloride
CHEBI_86345["CHEBI:86345"]
ing0_Magnesium_chloride -.-> CHEBI_86345
ing1_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007261 --> ing1_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing1_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing2_Boric_acid["Boric acid"]
CultureMech_007261 --> ing2_Boric_acid
CHEBI_33118["CHEBI:33118"]
ing2_Boric_acid -.-> CHEBI_33118
ing3_Calcium_sulfate["Calcium sulfate"]
CultureMech_007261 --> ing3_Calcium_sulfate
CHEBI_31346["CHEBI:31346"]
ing3_Calcium_sulfate -.-> CHEBI_31346
ing4_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
CultureMech_007261 --> ing4_Dibasic_sodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing4_Dibasic_sodium_phosphate -.-> CHEBI_34683
ing5_Sodium_molybdate["Sodium molybdate"]
CultureMech_007261 --> ing5_Sodium_molybdate
CHEBI_75215["CHEBI:75215"]
ing5_Sodium_molybdate -.-> CHEBI_75215
ing6_Cobaltous_sulfate["Cobaltous sulfate"]
CultureMech_007261 --> ing6_Cobaltous_sulfate
CHEBI_53470["CHEBI:53470"]
ing6_Cobaltous_sulfate -.-> CHEBI_53470
ing7_Sodium_chloride["Sodium chloride"]
CultureMech_007261 --> ing7_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing7_Sodium_chloride -.-> CHEBI_26710
ing8_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007261 --> ing8_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing8_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing9_Manganese_sulfate["Manganese sulfate"]
CultureMech_007261 --> ing9_Manganese_sulfate
CHEBI_86364["CHEBI:86364"]
ing9_Manganese_sulfate -.-> CHEBI_86364
ing10_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007261 --> ing10_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing10_Ferrous_sulfate -.-> CHEBI_75832
ing11_Zinc_sulfate["Zinc sulfate"]
CultureMech_007261 --> ing11_Zinc_sulfate
CHEBI_35176["CHEBI:35176"]
ing11_Zinc_sulfate -.-> CHEBI_35176
ing12_Ammonium_sulfate["Ammonium sulfate"]
CultureMech_007261 --> ing12_Ammonium_sulfate
CHEBI_62946["CHEBI:62946"]
ing12_Ammonium_sulfate -.-> CHEBI_62946
ing13_Cupric_sulfate["Cupric sulfate"]
CultureMech_007261 --> ing13_Cupric_sulfate
CHEBI_23414["CHEBI:23414"]
ing13_Cupric_sulfate -.-> CHEBI_23414
ing14_Sodium_citrate["Sodium citrate"]
CultureMech_007261 --> ing14_Sodium_citrate
CHEBI_53258["CHEBI:53258"]
ing14_Sodium_citrate -.-> CHEBI_53258
ing15__Iron_III["'Iron(III"]
CultureMech_007261 --> ing15__Iron_III
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
|
5.0 MILLIMOLAR |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 2 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.01 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 3 |
Boric acid
H3BO3 61.833 g/mol
|
8.086e-05 MILLIMOLAR |
CHEBI:33118
boric acid
|
|
| 4 |
Calcium sulfate
Ca.O4S 136.141 g/mol
|
0.001469 MILLIMOLAR |
CHEBI:31346
calcium sulfate
|
|
| 5 |
Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
|
3.37 MILLIMOLAR |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 6 |
Sodium molybdate
MoO4.2Na 205.916 g/mol
|
4.856e-05 MILLIMOLAR |
CHEBI:75215
sodium molybdate (anhydrous)
|
|
| 7 |
Cobaltous sulfate
Co.O4S 154.996 g/mol
|
3.557e-05 MILLIMOLAR |
CHEBI:53470
cobalt(2+) sulfate
|
|
| 8 |
Sodium chloride
Cl.Na 58.443 g/mol
|
179.0 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 9 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
2.2 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 10 |
Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
|
0.0001325 MILLIMOLAR |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 11 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.0007194 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 12 |
Zinc sulfate
O4S.Zn 161.453 g/mol
|
6.955e-05 MILLIMOLAR |
CHEBI:35176
zinc sulfate
|
|
| 13 |
Ammonium sulfate
2H4N.O4S 132.141 g/mol
|
1.51 MILLIMOLAR |
CHEBI:62946
ammonium sulfate
|
|
| 14 |
Cupric sulfate
Cu.O4S 159.609 g/mol
|
0.0001253 MILLIMOLAR |
CHEBI:23414
copper(II) sulfate
|
|
| 15 |
Sodium citrate
C6H5O7.3Na 258.069 g/mol
|
0.1 MILLIMOLAR |
CHEBI:53258
sodium citrate
|
|
| 16 | 'Iron(III | 0.001 MILLIMOLAR |
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- minimal_medium_whitchurch_et_al (CultureMech:007261)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:42.833189Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 35, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-01-28T19:48:42.930249Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:47.254214Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007261 |
| 2026-03-17T06:30:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:05.462530+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Minimal medium; whitchurch et al" to "minimal_medium_whitchurch_et_al" for consistent matching |
| 2026-06-05T07:41:34.756148+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:46.454811+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 8838205e6374c00fcd501f1de0cf03e485d7751f84029650f765f8f566b390b3 |