minimal_medium_whitchurch_et_al

CultureMech:007261 bacterial DEFINED LIQUID
Original name: Minimal medium; whitchurch et al

Overview

External ontology
MEDIADB:35 (source link unavailable) — Minimal medium; whitchurch et al
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
High-metal flag
True
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007261["minimal_medium_whitchurch_et_al"]
    ing0_Magnesium_chloride["Magnesium chloride"]
    CultureMech_007261 --> ing0_Magnesium_chloride
    CHEBI_86345["CHEBI:86345"]
    ing0_Magnesium_chloride -.-> CHEBI_86345
    ing1_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007261 --> ing1_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing1_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing2_Boric_acid["Boric acid"]
    CultureMech_007261 --> ing2_Boric_acid
    CHEBI_33118["CHEBI:33118"]
    ing2_Boric_acid -.-> CHEBI_33118
    ing3_Calcium_sulfate["Calcium sulfate"]
    CultureMech_007261 --> ing3_Calcium_sulfate
    CHEBI_31346["CHEBI:31346"]
    ing3_Calcium_sulfate -.-> CHEBI_31346
    ing4_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
    CultureMech_007261 --> ing4_Dibasic_sodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing4_Dibasic_sodium_phosphate -.-> CHEBI_34683
    ing5_Sodium_molybdate["Sodium molybdate"]
    CultureMech_007261 --> ing5_Sodium_molybdate
    CHEBI_75215["CHEBI:75215"]
    ing5_Sodium_molybdate -.-> CHEBI_75215
    ing6_Cobaltous_sulfate["Cobaltous sulfate"]
    CultureMech_007261 --> ing6_Cobaltous_sulfate
    CHEBI_53470["CHEBI:53470"]
    ing6_Cobaltous_sulfate -.-> CHEBI_53470
    ing7_Sodium_chloride["Sodium chloride"]
    CultureMech_007261 --> ing7_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing7_Sodium_chloride -.-> CHEBI_26710
    ing8_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007261 --> ing8_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing8_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing9_Manganese_sulfate["Manganese sulfate"]
    CultureMech_007261 --> ing9_Manganese_sulfate
    CHEBI_86364["CHEBI:86364"]
    ing9_Manganese_sulfate -.-> CHEBI_86364
    ing10_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007261 --> ing10_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing10_Ferrous_sulfate -.-> CHEBI_75832
    ing11_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007261 --> ing11_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing11_Zinc_sulfate -.-> CHEBI_35176
    ing12_Ammonium_sulfate["Ammonium sulfate"]
    CultureMech_007261 --> ing12_Ammonium_sulfate
    CHEBI_62946["CHEBI:62946"]
    ing12_Ammonium_sulfate -.-> CHEBI_62946
    ing13_Cupric_sulfate["Cupric sulfate"]
    CultureMech_007261 --> ing13_Cupric_sulfate
    CHEBI_23414["CHEBI:23414"]
    ing13_Cupric_sulfate -.-> CHEBI_23414
    ing14_Sodium_citrate["Sodium citrate"]
    CultureMech_007261 --> ing14_Sodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing14_Sodium_citrate -.-> CHEBI_53258
    ing15_Iron_III__chloride["Iron(III) chloride"]
    CultureMech_007261 --> ing15_Iron_III__chloride

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
5.0 MILLIMOLAR CHEBI:86345
magnesium dichloride hexahydrate
2 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.01 MILLIMOLAR CHEBI:3312
calcium dichloride
3 Boric acid
H3BO3 61.833 g/mol
8.086e-05 MILLIMOLAR CHEBI:33118
boric acid
4 Calcium sulfate
Ca.O4S 136.141 g/mol
0.001469 MILLIMOLAR CHEBI:31346
calcium sulfate
5 Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
3.37 MILLIMOLAR CHEBI:34683
disodium hydrogenphosphate
6 Sodium molybdate
MoO4.2Na 205.916 g/mol
4.856e-05 MILLIMOLAR CHEBI:75215
sodium molybdate (anhydrous)
7 Cobaltous sulfate
Co.O4S 154.996 g/mol
3.557e-05 MILLIMOLAR CHEBI:53470
cobalt(2+) sulfate
8 Sodium chloride
Cl.Na 58.443 g/mol
179.0 MILLIMOLAR CHEBI:26710
sodium chloride
9 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
2.2 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
10 Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
0.0001325 MILLIMOLAR CHEBI:86364
manganese(II) sulfate monohydrate
11 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.0007194 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
12 Zinc sulfate
O4S.Zn 161.453 g/mol
6.955e-05 MILLIMOLAR CHEBI:35176
zinc sulfate
13 Ammonium sulfate
2H4N.O4S 132.141 g/mol
1.51 MILLIMOLAR CHEBI:62946
ammonium sulfate
14 Cupric sulfate
Cu.O4S 159.609 g/mol
0.0001253 MILLIMOLAR CHEBI:23414
copper(II) sulfate
15 Sodium citrate
C6H5O7.3Na 258.069 g/mol
0.1 MILLIMOLAR CHEBI:53258
sodium citrate
16 Iron(III) chloride 0.001 MILLIMOLAR

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:42.833189Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 35, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:42.930249Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:47.254214Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007261
2026-03-17T06:30:57.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:05.462530+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Minimal medium; whitchurch et al" to "minimal_medium_whitchurch_et_al" for consistent matching
2026-06-05T07:41:34.756148+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-31T04:07:02.657128+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored 1 ingredient name(s) truncated at a parenthesis by the MediaDB SQL parser. Resolved against MEDIADB:35's own compound list in media_database.07Oct2015.sql, requiring a unique prefix match and, where more than one matched, an equal Amount_mM.