Overview
Composition
flowchart LR
CultureMech_007453["1063"]
ing0_Tryptone5gYeast_extract3gNaCl10gUrea1gDistilled_water1LAgar__if_needed_15gAdjust_pH_to_8_5_with_50_mM_Tris_HCl_buffer_["Tryptone5gYeast extract3gNaCl10gUrea1gDistilled water1LAgar (if nee..."]
CultureMech_007453 --> ing0_Tryptone5gYeast_extract3gNaCl10gUrea1gDistilled_water1LAgar__if_needed_15gAdjust_pH_to_8_5_with_50_mM_Tris_HCl_buffer_
ing1__["?"]
CultureMech_007453 --> ing1__
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone5gYeast extract3gNaCl10gUrea1gDistilled water1LAgar (if needed)15gAdjust pH to 8.5 with 50 mM Tris-HCl buffer. | variable VARIABLE | ||
| 2 | Tryptone G_PER_L |
Preparation
- Dissolve ingredients in distilled water
- Adjust pH if needed
- Sterilize by autoclaving
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- NBRC_1063 (CultureMech:007453)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-02-20T00:22:36.015984Z | nbrc-import | Imported from NBRC | Source: NBRC, Media No. 1062, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=1062. Imported via ethical web scraping. |
| 2026-03-13T03:09:36.095728Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 6 duplicate ingredient(s) |
| 2026-03-13T06:19:49.131924Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007453 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Tryptone5gYeast extract3gNaCl10gUrea1gDistilled water1LAgar (if needed)15gAdjust pH to 8.5 with 50 mM Tris-HCl buffer. |
| 2026-05-16T09:03:06.569585+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-08-06T03:05:04.601919+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3c8ab53303e6001d27f76d71cd748ffc9094dd2ea4324c5eb76dcd6e59da84ae |