Pae I Medium + 0.1% (w/v) Urea

CultureMech:007453 bacterial COMPLEX SOLID_AGAR

Overview

External ontology
nbrc.medium:1062 (source link unavailable) — NBRC Medium 1062
pH
8.5
Notes
Source: NBRC Medium 1062 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1062 NBRC lists Pae I Medium with 0.1% (w/v) urea, tryptone, yeast extract, NaCl, optional agar, and adjustment to pH 8.5 with 50 mM Tris-HCl buffer.

Composition

flowchart LR
    CultureMech_007453["Pae I Medium + 0.1% (w/v) Urea"]
    ing0_Tryptone["Tryptone"]
    CultureMech_007453 --> ing0_Tryptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_007453 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_007453 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_Urea["Urea"]
    CultureMech_007453 --> ing3_Urea
    CHEBI_16199["CHEBI:16199"]
    ing3_Urea -.-> CHEBI_16199
    ing4_Distilled_water["Distilled water"]
    CultureMech_007453 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377
    ing5_Agar__if_needed_["Agar (if needed)"]
    CultureMech_007453 --> ing5_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar__if_needed_ -.-> CHEBI_2509
    ing6_Tris_HCl_buffer["Tris-HCl buffer"]
    CultureMech_007453 --> ing6_Tris_HCl_buffer
    CHEBI_9754["CHEBI:9754"]
    ing6_Tris_HCl_buffer -.-> CHEBI_9754

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Tryptone 5 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 NBRC Medium 1062 lists 5 g tryptone.
2 Yeast extract 3 G_PER_L FOODON:03315426
yeast extract
 NBRC Medium 1062 lists 3 g yeast extract.
3 NaCl
Cl.Na 58.443 g/mol
10 G_PER_L CHEBI:26710
sodium chloride
 NBRC Medium 1062 lists 10 g NaCl.
4 Urea
CH4N2O 60.056 g/mol
1 G_PER_L CHEBI:16199
urea
 NBRC Medium 1062 lists 1 g urea.
5 Distilled water
H2O 18.015 g/mol
1 L CHEBI:15377
water
 NBRC Medium 1062 lists 1 L distilled water.
6 Agar (if needed) 15 G_PER_L CHEBI:2509
agar
 NBRC Medium 1062 lists 15 g agar if needed.
7 Tris-HCl buffer
C4H11NO3 121.136 g/mol
variable VARIABLE CHEBI:9754
tris
 NBRC Medium 1062 adjusts the medium to pH 8.5 with 50 mM Tris-HCl buffer.

Preparation

  1. Dissolve tryptone, yeast extract, NaCl, urea, and agar if needed in 1 L distilled water.
  2. Adjust pH to 8.5 with 50 mM Tris-HCl buffer.
  3. Sterilize by autoclaving.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-02-20T00:22:36.015984Z nbrc-import Imported from NBRC Source: NBRC, Media No. 1062, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=1062. Imported via ethical web scraping.
2026-03-13T03:09:36.095728Z data-quality-cleanup-v1.0 Data quality fixes Merged 6 duplicate ingredient(s)
2026-03-13T06:19:49.131924Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007453
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Tryptone5gYeast extract3gNaCl10gUrea1gDistilled water1LAgar (if needed)15gAdjust pH to 8.5 with 50 mM Tris-HCl buffer.
2026-05-16T09:03:06.569585+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-08-19T21:54:22.561094+00:00 recover_nbrc_composition.py Recovered composition from source HTML Re-parsed the NBRC medium 1062 composition table from the preserved scrape; 6 ingredient(s) restored, 0 stock solution(s) nested rather than flattened, 0 unparsed solution blob(s) removed (#299).
2026-08-20T19:01:15.731395+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 3 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-09-11T00:00:00-07:00 repair_nbrc_1063_score15.py RESOLVED_NBRC_1063_SCORE15 Added the NBRC Medium 1062 source term and official title, grounded tryptone, yeast extract, agar, and Tris-HCl buffer, and recorded pH 8.5.