Overview
- Notes
- Commercial Product: MacConkey Agar Supplier: Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) Catalog: BD 212123, Sigma 05465 Source: https://microbenotes.com/macconkey-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_007470["1298"]
ing0_Peptone["Peptone"]
CultureMech_007470 --> ing0_Peptone
ing1_Proteose_peptone["Proteose peptone"]
CultureMech_007470 --> ing1_Proteose_peptone
ing2_Lactose_monohydrate["Lactose monohydrate"]
CultureMech_007470 --> ing2_Lactose_monohydrate
CHEBI_17716["CHEBI:17716"]
ing2_Lactose_monohydrate -.-> CHEBI_17716
ing3_Bile_salts["Bile salts"]
CultureMech_007470 --> ing3_Bile_salts
CHEBI_22868["CHEBI:22868"]
ing3_Bile_salts -.-> CHEBI_22868
ing4_Sodium_chloride["Sodium chloride"]
CultureMech_007470 --> ing4_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing4_Sodium_chloride -.-> CHEBI_26710
ing5_Neutral_red["Neutral red"]
CultureMech_007470 --> ing5_Neutral_red
CHEBI_86370["CHEBI:86370"]
ing5_Neutral_red -.-> CHEBI_86370
ing6_Crystal_violet["Crystal violet"]
CultureMech_007470 --> ing6_Crystal_violet
CHEBI_41688["CHEBI:41688"]
ing6_Crystal_violet -.-> CHEBI_41688
ing7_Agar["Agar"]
CultureMech_007470 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
ing8__["?"]
CultureMech_007470 --> ing8__
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Peptone
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
17.0 G_PER_L |
MICRO:0000178 (source link unavailable)
peptone
|
Pancreatic digest of gelatin, provides nitrogen and amino acids; Constituent of MacConkey Agar commercial product |
| 2 |
Proteose peptone
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
3.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Meat and casein digest, provides additional nutrients; Constituent of MacConkey Agar commercial product |
| 3 |
Lactose monohydrate
C12H22O11 342.297 g/mol
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
10.0 G_PER_L |
CHEBI:17716
lactose
|
Fermentable carbohydrate for differentiation. Fermentation produces acid causing pH drop and color change; Constituent of MacConkey Agar commercial product |
| 4 |
Bile salts
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
1.5 G_PER_L |
CHEBI:22868
bile salt
|
Selective agent - inhibits gram-positive bacteria; Constituent of MacConkey Agar commercial product |
| 5 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of MacConkey Agar commercial product |
| 6 |
Neutral red
C15H16N4.HCl 288.782 g/mol
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
0.03 G_PER_L |
CHEBI:86370
neutral red
|
pH indicator - turns red/pink below pH 6.8, absorbed by colonies of lactose fermenters; Constituent of MacConkey Agar commercial product |
| 7 |
Crystal violet
C25H30N3.Cl 407.989 g/mol
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
0.001 G_PER_L |
CHEBI:41688
crystal violet
|
Selective agent - inhibits gram-positive bacteria; Constituent of MacConkey Agar commercial product |
| 8 |
Agar
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
13.5 G_PER_L |
CHEBI:2509
agar
|
Solidifying agent; Constituent of MacConkey Agar commercial product |
| 9 | Nutrient Broth Powder (Kyokuto*) G_PER_L |
Preparation
- Dissolve ingredients in distilled water
- Adjust pH if needed
- Sterilize by autoclaving
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- NBRC_1298 (CultureMech:007470)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-02-20T00:22:36.053461Z | nbrc-import | Imported from NBRC | Source: NBRC, Media No. 1296, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=1296. Imported via ethical web scraping. |
| 2026-03-13T03:08:46.763759Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 6 duplicate ingredient(s) |
| 2026-03-13T06:19:49.182736Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007470 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Nutrient Broth Powder (Kyokuto*)6gPyruvic acid sodium salt1gDistilled water1LAgar (if needed)15gpH 7.0*Kyokuto Pharmaceutical Industrial Co., LTD., Code; 551-02480-7 |
| 2026-05-16T09:03:06.631668+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-05-17T07:06:16.028719+00:00 | cleanup_residual_errors.py | CLEANUP_RESIDUAL_ERRORS | R01_flatten_synonym_text=2 |
| 2026-08-06T03:05:04.631589+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint e07afd5834a3f9851f9deeac1cb9b332d21f3ba5e3dce208b54a3d2ac5f03f5a |