NBRC Medium 1296

CultureMech:007470 bacterial COMPLEX SOLID_AGAR

Overview

External ontology
nbrc.medium:1296 (source link unavailable) — NBRC Medium 1296
pH
7.0
Notes
Source: NBRC Medium 1296 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1296 NBRC lists 6 g Nutrient Broth Powder from Kyokuto Pharmaceutical Industrial Co., LTD. (Code 551-02480-7), 1 g pyruvic acid sodium salt, 1 L distilled water, optional agar, and pH 7.0.

Composition

flowchart LR
    CultureMech_007470["NBRC Medium 1296"]
    ing0_Nutrient_Broth_Powder__Kyokuto__["Nutrient Broth Powder (Kyokuto*)"]
    CultureMech_007470 --> ing0_Nutrient_Broth_Powder__Kyokuto__
    ing1_Pyruvic_acid_sodium_salt["Pyruvic acid sodium salt"]
    CultureMech_007470 --> ing1_Pyruvic_acid_sodium_salt
    CHEBI_50144["CHEBI:50144"]
    ing1_Pyruvic_acid_sodium_salt -.-> CHEBI_50144
    ing2_Distilled_water["Distilled water"]
    CultureMech_007470 --> ing2_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing2_Distilled_water -.-> CHEBI_15377
    ing3_Agar__if_needed_["Agar (if needed)"]
    CultureMech_007470 --> ing3_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar__if_needed_ -.-> CHEBI_2509

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Nutrient Broth Powder (Kyokuto*) 6 G_PER_L NBRC Medium 1296 lists 6 g Nutrient Broth Powder from Kyokuto Pharmaceutical Industrial Co., LTD., Code 551-02480-7.
2 Pyruvic acid sodium salt
C3H3O3.Na 110.044 g/mol
1 G_PER_L CHEBI:50144
sodium pyruvate
 NBRC Medium 1296 lists 1 g pyruvic acid sodium salt.
3 Distilled water
H2O 18.015 g/mol
1 L CHEBI:15377
water
 NBRC Medium 1296 lists 1 L distilled water.
4 Agar (if needed) 15 G_PER_L CHEBI:2509
agar
 NBRC Medium 1296 lists 15 g agar if needed.

Preparation

  1. Dissolve Nutrient Broth Powder, pyruvic acid sodium salt, and agar if needed in 1 L distilled water.
  2. Adjust pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-02-20T00:22:36.053461Z nbrc-import Imported from NBRC Source: NBRC, Media No. 1296, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=1296. Imported via ethical web scraping.
2026-03-13T03:08:46.763759Z data-quality-cleanup-v1.0 Data quality fixes Merged 6 duplicate ingredient(s)
2026-03-13T06:19:49.182736Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007470
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Nutrient Broth Powder (Kyokuto*)6gPyruvic acid sodium salt1gDistilled water1LAgar (if needed)15gpH 7.0*Kyokuto Pharmaceutical Industrial Co., LTD., Code; 551-02480-7
2026-05-16T09:03:06.631668+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-05-17T07:06:16.028719+00:00 cleanup_residual_errors.py CLEANUP_RESIDUAL_ERRORS R01_flatten_synonym_text=2
2026-08-19T21:54:22.732836+00:00 recover_nbrc_composition.py Removed corrupt rows, kept curated composition Record already held a grounded ingredient list, so the NBRC medium 1296 page was NOT used — it lists only the commercial premix. Dropped the empty-name row and 0 unparsed solution blob(s) (#299).
2026-09-11T00:00:00-07:00 repair_nbrc_1298_score15.py RESOLVED_NBRC_1298_SCORE15 Replaced MacConkey Agar constituent rows with NBRC Medium 1296's Nutrient Broth Powder, pyruvic acid sodium salt, water, optional agar, and pH 7.0 formula.