869

CultureMech:007500 bacterial DEFINED SOLID_AGAR

Overview

Composition

flowchart LR
    CultureMech_007500["869"]
    ing0_Bacto_Marine_Broth_2216__Difco_7_5gSodium_pyruvate5gAgar__if_needed_15gSea_water_800mlDistilled_water200mlpH_7_6_Filtered__aged_seawater_or_artificial_seawater__Daigo_s_Artificial_Seawater_SP_____Wako_Pure_Chemical_Ind___Ltd___Osaka__Japan_["Bacto Marine Broth 2216 (Difco)7.5gSodium pyruvate5gAgar (if needed..."]
    CultureMech_007500 --> ing0_Bacto_Marine_Broth_2216__Difco_7_5gSodium_pyruvate5gAgar__if_needed_15gSea_water_800mlDistilled_water200mlpH_7_6_Filtered__aged_seawater_or_artificial_seawater__Daigo_s_Artificial_Seawater_SP_____Wako_Pure_Chemical_Ind___Ltd___Osaka__Japan_
    ing1__["?"]
    CultureMech_007500 --> ing1__

Ingredients (2)

# Ingredient Concentration Ontology Notes
1 Bacto Marine Broth 2216 (Difco)7.5gSodium pyruvate5gAgar (if needed)15gSea water*800mlDistilled water200mlpH 7.6*Filtered, aged seawater or artificial seawater (Daigo's Artificial Seawater SP**)**Wako Pure Chemical Ind., Ltd., Osaka, Japan. variable VARIABLE
2 Bacto Marine Broth 2216 (Difco) G_PER_L

Preparation

  1. Dissolve ingredients in distilled water
  2. Adjust pH if needed
  3. Sterilize by autoclaving

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-02-20T00:22:35.979642Z nbrc-import Imported from NBRC Source: NBRC, Media No. 868, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=868. Imported via ethical web scraping.
2026-03-13T03:09:24.689761Z data-quality-cleanup-v1.0 Data quality fixes Merged 9 duplicate ingredient(s)
2026-03-13T06:19:49.270356Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007500
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Bacto Marine Broth 2216 (Difco)7.5gSodium pyruvate5gAgar (if needed)15gSea water*800mlDistilled water200mlpH 7.6*Filtered, aged seawater or artificial seawater (Daigo's Artificial Seawater SP**)**Wako Pure Chemical Ind., Ltd., Osaka, Japan.
2026-05-16T09:03:06.732868+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-08-06T03:05:04.690811+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint dbc30c4aa7188347d2ae0739be89ac9e0fb3eb7a74e9b8da528a59e336e3353f