869

CultureMech:007500 bacterial DEFINED SOLID_AGAR

Overview

External ontology
nbrc.medium:868 (source link unavailable) — NBRC Medium 868
pH
7.6
Notes
Source: NBRC Medium 868 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=868 The NBRC 1/5 Marine Broth + 0.5% Sodium Pyruvate table lists Bacto Marine Broth 2216, sodium pyruvate, optional agar, filtered aged seawater or Daigo's Artificial Seawater SP, and final pH 7.6.

Composition

flowchart LR
    CultureMech_007500["869"]
    ing0_Bacto_Marine_Broth_2216__Difco_["Bacto Marine Broth 2216 (Difco)"]
    CultureMech_007500 --> ing0_Bacto_Marine_Broth_2216__Difco_
    ing1_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_007500 --> ing1_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing1_Sodium_pyruvate -.-> CHEBI_50144
    ing2_Agar__if_needed_["Agar (if needed)"]
    CultureMech_007500 --> ing2_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar__if_needed_ -.-> CHEBI_2509
    ing3_Sea_water_["Sea water*"]
    CultureMech_007500 --> ing3_Sea_water_
    ing4_Distilled_water["Distilled water"]
    CultureMech_007500 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Bacto Marine Broth 2216 (Difco) 7.5 G_PER_L
2 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
5 G_PER_L CHEBI:50144
Na-pyruvate
3 Agar (if needed) 15 G_PER_L CHEBI:2509
Agar
4 Sea water* 800 ML_PER_L
5 Distilled water
H2O 18.015 g/mol
200 ML_PER_L CHEBI:15377
water

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-02-20T00:22:35.979642Z nbrc-import Imported from NBRC Source: NBRC, Media No. 868, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=868. Imported via ethical web scraping.
2026-03-13T03:09:24.689761Z data-quality-cleanup-v1.0 Data quality fixes Merged 9 duplicate ingredient(s)
2026-03-13T06:19:49.270356Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007500
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Bacto Marine Broth 2216 (Difco)7.5gSodium pyruvate5gAgar (if needed)15gSea water*800mlDistilled water200mlpH 7.6*Filtered, aged seawater or artificial seawater (Daigo's Artificial Seawater SP**)**Wako Pure Chemical Ind., Ltd., Osaka, Japan.
2026-05-16T09:03:06.732868+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-08-19T21:54:23.018314+00:00 recover_nbrc_composition.py Recovered composition from source HTML Re-parsed the NBRC medium 868 composition table from the preserved scrape; 5 ingredient(s) restored, 0 stock solution(s) nested rather than flattened, 0 unparsed solution blob(s) removed (#299).
2026-08-20T19:01:16.102776+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-09-07T00:00:00-07:00 repair_nbrc_partial_score25.py RESOLVED_NBRC_PARTIAL_SCORE25 Preserved the recovered NBRC Medium 868 composition table.