Overview
- External ontology
- TOGO:M1050 — M30
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1050 Original source: JCM - JCM_M995-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=995
Composition
flowchart LR
CultureMech_007567["m30"]
ing0_0_1_M_Tris__HCl_buffer__pH_7_5_["0.1 M Tris--HCl buffer (pH 7.5)"]
CultureMech_007567 --> ing0_0_1_M_Tris__HCl_buffer__pH_7_5_
ing1_Distilled_water["Distilled water"]
CultureMech_007567 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_gelrite__Gellan_Gum_["gelrite (Gellan Gum)"]
CultureMech_007567 --> ing2_gelrite__Gellan_Gum_
ing3_Aged_seawater["Aged seawater"]
CultureMech_007567 --> ing3_Aged_seawater
ing4_Ampicillin__50_mg_ml_["Ampicillin (50 mg/ml)"]
CultureMech_007567 --> ing4_Ampicillin__50_mg_ml_
ing5_Na2HPO4_2H2O["Na2HPO4・2H2O"]
CultureMech_007567 --> ing5_Na2HPO4_2H2O
CHEBI_34683["CHEBI:34683"]
ing5_Na2HPO4_2H2O -.-> CHEBI_34683
ing6_N__Acetyl__D__glucosamine__Sigma_["N--Acetyl--D--glucosamine (Sigma)"]
CultureMech_007567 --> ing6_N__Acetyl__D__glucosamine__Sigma_
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | 0.1 M Tris--HCl buffer (pH 7.5) | 50 G_PER_L | Role: Buffer; Properties: Complex component, Defined component, Solution | |
| 2 |
Distilled water
H2O 18.015 g/mol
|
300.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 260.0, 40.0] |
| 3 | gelrite (Gellan Gum) | 5 G_PER_L | Role: Solidifying component; Properties: Organic compound, Undefined component | |
| 4 | Aged seawater | 700 G_PER_L | Role: Solvating media; Properties: Undefined component, Complex component | |
| 5 | Ampicillin (50 mg/ml) | 4 G_PER_L | Role: Antimicrobial agent; Properties: Defined component, Organic compound, Solution | |
| 6 |
Na2HPO4・2H2O
HO4P.2Na 141.958 g/mol
|
0.01 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 7 | N--Acetyl--D--glucosamine (Sigma) | 2 G_PER_L | Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
Solutions
Solution 1
Dose: 1030 G_PER_L
See mediadive_6108_Solution_1.yaml for composition
Solution 2
Dose: 0.2 G_PER_L
See mediadive_6109_Solution_2.yaml for composition
Modified Hutner's basal salts (see Medium [M941])
Dose: 20 G_PER_L
Cross-reference to Medium M941
Vitamin solution No. 6 (see Medium [M1049])
Dose: 10 G_PER_L
Cross-reference to Medium M1049
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1050_M30 (CultureMech:007567)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.829434Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1050 |
| 2026-01-28T19:48:20.619514Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:34.168798+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:09:03.711217Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:49.729162Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007567 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:09.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:08.079852+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M30" to "m30" for consistent matching |
| 2026-05-16T09:03:07.199400+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:30.026363+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:05.242861+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint f139279c1f57d0275e0b1669dbf420e7c0e502c57c6651fb7438d5916edf23a4 |