Overview
- External ontology
- TOGO:M1050 — M30
- Applications
-
- Microbial cultivation
- Notes
- TOGO M1050 records the solid JCM Medium 995 M30 variant: 1030 ml Solution 1 plus 0.2 ml filter-sterilized Solution 2. Solution 1 contains 5 g/L gelrite, aged seawater, Tris-HCl buffer, distilled water, and Modified Hutner's basal salts from M941/JCM 900; Solution 2 contains 50 mg/ml Ampicillin, Na2HPO4 x 2H2O, N-Acetyl-D-glucosamine, and Vitamin solution No. 6 from M1049/JCM 995.
Ingredients (0)
No ingredients recorded.
Preparation
- Prepare Solution 1 from Tris-HCl buffer, distilled water, gelrite, aged seawater, and Modified Hutner's basal salts.
- Prepare Solution 2 from 50 mg/ml Ampicillin, distilled water, Na2HPO4 x 2H2O, N-Acetyl-D-glucosamine, and Vitamin solution No. 6.
- Filter-sterilize Solution 2.
- Add 0.2 ml filter-sterilized Solution 2 to Solution 1.
Solutions
Solution 1
Dose: 1030 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| 0.1 M Tris-HCl buffer (pH 7.5) | 50.0 ML_PER_L | TOGO M1050 / JCM Medium 995 lists 50.0 ml/L 0.1 M Tris-HCl buffer (pH 7.5). |
| Distilled water | 260.0 ML_PER_L | TOGO M1050 / JCM Medium 995 lists 260.0 ml/L Distilled water. |
| gelrite (Gellan Gum) | 5.0 G_PER_L | TOGO M1050 / JCM Medium 995 lists 5.0 g/L gelrite (Gellan Gum). |
| Aged seawater | 700.0 ML_PER_L | TOGO M1050 / JCM Medium 995 lists 700.0 ml/L Aged seawater. |
| Modified Hutner's basal salts | 20.0 ML_PER_L | TOGO M1050 / JCM Medium 995 adds 20.0 ml/L Modified Hutner's basal salts from TOGO M941. |
TOGO M1050 / JCM Medium 995 combines 1030 ml Solution 1 with 0.2 ml filter-sterilized Solution 2.
Solution 2
Dose: 0.2 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Ampicillin (50 mg/ml) | variable VARIABLE | TOGO M1050 / JCM Medium 995 lists 4.0 ml Ampicillin (50 mg/ml) in Solution 2. |
| Distilled water | variable VARIABLE | TOGO M1050 / JCM Medium 995 lists 40.0 ml Distilled water in Solution 2. |
| Na2HPO4 x 2H2O | variable VARIABLE | TOGO M1050 / JCM Medium 995 lists 0.01 g Na2HPO4 x 2H2O in Solution 2. |
| N-Acetyl-D-glucosamine (Sigma) | variable VARIABLE | TOGO M1050 / JCM Medium 995 lists 2.0 g N-Acetyl-D-glucosamine (Sigma) in Solution 2. |
| Vitamin solution No. 6 | variable VARIABLE | TOGO M1050 / JCM Medium 995 lists 10.0 ml Vitamin solution No. 6 in Solution 2. |
TOGO M1050 / JCM Medium 995 adds 0.2 ml filter-sterilized Solution 2 to Solution 1.
Modified Hutner's basal salts
Dose: 20.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 950.0 ML_PER_L | TOGO M941 / JCM Medium 900 lists 950.0 ml/L Distilled water. |
| Nitrilotriacetic acid | 10.0 G_PER_L | TOGO M941 / JCM Medium 900 lists 10.0 g/L Nitrilotriacetic acid. |
| MgSO4 x 7H2O | 29.7 G_PER_L | TOGO M941 / JCM Medium 900 lists 29.7 g/L MgSO4 x 7H2O. |
| CaCl2 x 2H2O | 3.335 G_PER_L | TOGO M941 / JCM Medium 900 lists 3.335 g/L CaCl2 x 2H2O. |
| (NH4)6Mo7O24 x 4H2O | 9.25 MG_PER_L | TOGO M941 / JCM Medium 900 lists 9.25 mg/L (NH4)6Mo7O24 x 4H2O. |
| FeSO4 x 7H2O | 99.0 MG_PER_L | TOGO M941 / JCM Medium 900 lists 99.0 mg/L FeSO4 x 7H2O. |
| Metals "44" | 50.0 ML_PER_L | TOGO M941 / JCM Medium 900 lists 50.0 ml/L Metals "44". |
| KOH | variable VARIABLE | TOGO M941 / JCM Medium 900 dissolves nitrilotriacetic acid and adjusts to pH 7.0 with about 7.3 g KOH before adding the remaining salts. |
| NaOH | variable VARIABLE | TOGO M941 / JCM Medium 900 readjusts Modified Hutner's basal salts to pH 6.8 with NaOH or H2SO4 as needed. |
| H2SO4 | variable VARIABLE | TOGO M941 / JCM Medium 900 readjusts Modified Hutner's basal salts to pH 6.8 with NaOH or H2SO4 as needed. |
TOGO M1050 / JCM Medium 995 adds 20.0 ml/L Modified Hutner's basal salts from TOGO M941.
Metals "44"
Dose: 50.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 1.0 L | TOGO M140 / JCM Medium 149 lists 1.0 L Distilled water. |
| EDTA x 2Na | 250.0 MG_PER_L | TOGO M140 / JCM Medium 149 lists 250.0 mg/L EDTA x 2Na. |
| ZnSO4 x 7H2O | 1095.0 MG_PER_L | TOGO M140 / JCM Medium 149 lists 1095.0 mg/L ZnSO4 x 7H2O. |
| FeSO4 x 7H2O | 500.0 MG_PER_L | TOGO M140 / JCM Medium 149 lists 500.0 mg/L FeSO4 x 7H2O. |
| MnSO4 x H2O | 154.0 MG_PER_L | TOGO M140 / JCM Medium 149 lists 154.0 mg/L MnSO4 x H2O. |
| CuSO4 x 5H2O | 39.2 MG_PER_L | TOGO M140 / JCM Medium 149 lists 39.2 mg/L CuSO4 x 5H2O. |
| Co(NO3)2 x 6H2O | 24.8 MG_PER_L | TOGO M140 / JCM Medium 149 lists 24.8 mg/L Co(NO3)2 x 6H2O. |
| Na2B4O7 x 10H2O | 17.7 MG_PER_L | TOGO M140 / JCM Medium 149 lists 17.7 mg/L Na2B4O7 x 10H2O. |
TOGO M941 / JCM Medium 900 adds 50.0 ml/L Metals 44 from TOGO M140.
Vitamin solution No. 6
Dose: variable VARIABLE
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 1.0 L | TOGO M1049 / JCM Medium 995 lists 1.0 L Distilled water. |
| Biotin | 4.0 MG_PER_L | TOGO M1049 / JCM Medium 995 lists 4.0 mg/L Biotin. |
| Pyridoxine HCl | 20.0 MG_PER_L | TOGO M1049 / JCM Medium 995 lists 20.0 mg/L Pyridoxine HCl. |
| Thiamine HCl 2H2O | 10.0 MG_PER_L | TOGO M1049 / JCM Medium 995 lists 10.0 mg/L Thiamine HCl 2H2O. |
| Calcium pantothenate | 10.0 MG_PER_L | TOGO M1049 / JCM Medium 995 lists 10.0 mg/L Calcium pantothenate. |
| p-Aminobenzoic acid | 10.0 MG_PER_L | TOGO M1049 / JCM Medium 995 lists 10.0 mg/L p-Aminobenzoic acid. |
| Folic acid | 4.0 MG_PER_L | TOGO M1049 / JCM Medium 995 lists 4.0 mg/L Folic acid. |
| Riboflavin | 10.0 MG_PER_L | TOGO M1049 / JCM Medium 995 lists 10.0 mg/L Riboflavin. |
| Nicotinamide | 10.0 MG_PER_L | TOGO M1049 / JCM Medium 995 lists 10.0 mg/L Nicotinamide. |
| Vitamin B12 | 0.2 MG_PER_L | TOGO M1049 / JCM Medium 995 lists 0.2 mg/L Vitamin B12. |
TOGO M1050 / JCM Medium 995 lists 10.0 ml Vitamin solution No. 6 in Solution 2.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.829434Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1050 |
| 2026-01-28T19:48:20.619514Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:34.168798+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:09:03.711217Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:49.729162Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007567 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:09.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:08.079852+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M30" to "m30" for consistent matching |
| 2026-05-16T09:03:07.199400+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:30.026363+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m1050_score15.py | RESOLVED_TOGO_M1050_SCORE15 | TOGO M1050 records the solid JCM Medium 995 M30 variant: 1030 ml Solution 1 plus 0.2 ml filter-sterilized Solution 2. Solution 1 contains 5 g/L gelrite, aged seawater, Tris-HCl buffer, distilled water, and Modified Hutner's basal salts from M941/JCM 900; Solution 2 contains 50 mg/ml Ampicillin, Na2HPO4 x 2H2O, N-Acetyl-D-glucosamine, and Vitamin solution No. 6 from M1049/JCM 995. |