modified_halobacteria_medium_2

CultureMech:007581 archaea COMPLEX LIQUID
Original name: Modified Halobacteria Medium-2

Overview

External ontology
TOGO:M1064 — Modified Halobacteria Medium-2
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M1064 Original source: JCM - JCM_M1007 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1007

Composition

flowchart LR
    CultureMech_007581["modified_halobacteria_medium_2"]
    ing0_distilled_water["distilled water"]
    CultureMech_007581 --> ing0_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_007581 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_007581 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_KCl["KCl"]
    CultureMech_007581 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_MnCl2_4H2O["MnCl2・4H2O"]
    CultureMech_007581 --> ing4_MnCl2_4H2O
    CHEBI_86368["CHEBI:86368"]
    ing4_MnCl2_4H2O -.-> CHEBI_86368
    ing5_sodium_pyruvate["sodium pyruvate"]
    CultureMech_007581 --> ing5_sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing5_sodium_pyruvate -.-> CHEBI_50144
    ing6_Sodium_glutamate["Sodium glutamate"]
    CultureMech_007581 --> ing6_Sodium_glutamate
    CHEBI_64220["CHEBI:64220"]
    ing6_Sodium_glutamate -.-> CHEBI_64220
    ing7_Trisodium_citrate["Trisodium citrate"]
    CultureMech_007581 --> ing7_Trisodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing7_Trisodium_citrate -.-> CHEBI_53258
    ing8_FeCl2_4H2O["FeCl2・4H2O"]
    CultureMech_007581 --> ing8_FeCl2_4H2O
    CHEBI_86249["CHEBI:86249"]
    ing8_FeCl2_4H2O -.-> CHEBI_86249
    ing9_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_007581 --> ing9_Yeast_extract__BD_Difco_
    ing10_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
    CultureMech_007581 --> ing10_Casamino_acids__BD_Difco_

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
3 NaCl
Cl.Na 58.443 g/mol
200 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4 KCl
Cl.K 74.551 g/mol
2 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
5 MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
0.36 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
6 sodium pyruvate
C3H3O3.Na 110.044 g/mol
1 G_PER_L CHEBI:50144
sodium pyruvate
 Role: Carbon source; Properties: Simple component, Organic compound, Defined component
7 Sodium glutamate
C5H8NO4.Na 169.112 g/mol
1 G_PER_L CHEBI:64220
monosodium glutamate
 Role: Nitrogen source; Properties: Defined component, Simple component, Organic compound
8 Trisodium citrate
C6H5O7.3Na 258.069 g/mol
3 G_PER_L CHEBI:53258
sodium citrate
 Role: Buffer; Properties: Defined component, Simple component, Organic compound
9 FeCl2・4H2O
Cl2Fe.4H2O 198.811 g/mol
36 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
10 Yeast extract (BD-Difco) 5 G_PER_L Properties: Undefined component
11 Casamino acids (BD-Difco) 5 G_PER_L Properties: Undefined component

Solutions

Na2CO3 solution

Dose: variable VARIABLE

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:53.855558Z togo-import Imported from TOGO Medium Source: JCM, ID: M1064
2026-01-28T19:48:19.274719Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Na2CO3 solution
2026-03-09T08:04:31.892478+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:49.835904Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007581
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:06.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:08.187614+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified Halobacteria Medium-2" to "modified_halobacteria_medium_2" for consistent matching
2026-05-16T09:03:07.293857+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:30.126518+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:20.995904+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-08-06T03:04:24.451446+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 6e5daa600ec5d71c8e7c8f12bd0a2ae54e48ff7f3ac051119b02d44494d26736