Overview
- External ontology
- TOGO:M1064 — Modified Halobacteria Medium-2
- Applications
-
- Microbial cultivation
- pH
- 8.0
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1064 Original source: JCM - JCM_M1007 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1007 Source preparation excerpts: "Add components to distilled water and bring volume to 1.0 L."; "Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.". These instructions are not converted into an inferred step ordering. Separate JCM1007 excerpt: "After autoclaving, adjust pH to 8.0 with sterile Na2CO3 solution.". The modified recipe explicitly states 1.0 g/L MgSO4 heptahydrate (final), also reported as 1 g/L by TOGO M1064. Record this source-stated target, not 20 g/L copied from the base or an inferred 21 g/L sum. The word supplemented and the base recipe's 20 g per 1 L leave a preparation ambiguity: literal addition to the unchanged base cannot produce the stated lower target. This curation does not claim that operational ambiguity is resolved.
Composition
flowchart LR
CultureMech_007581["modified_halobacteria_medium_2"]
ing0_distilled_water["distilled water"]
CultureMech_007581 --> ing0_distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007581 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_007581 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_KCl["KCl"]
CultureMech_007581 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_007581 --> ing4_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing4_MnCl2_4H2O -.-> CHEBI_86368
ing5_sodium_pyruvate["sodium pyruvate"]
CultureMech_007581 --> ing5_sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing5_sodium_pyruvate -.-> CHEBI_50144
ing6_Sodium_glutamate["Sodium glutamate"]
CultureMech_007581 --> ing6_Sodium_glutamate
CHEBI_64220["CHEBI:64220"]
ing6_Sodium_glutamate -.-> CHEBI_64220
ing7_Trisodium_citrate["Trisodium citrate"]
CultureMech_007581 --> ing7_Trisodium_citrate
CHEBI_53258["CHEBI:53258"]
ing7_Trisodium_citrate -.-> CHEBI_53258
ing8_FeCl2_4H2O["FeCl2・4H2O"]
CultureMech_007581 --> ing8_FeCl2_4H2O
CHEBI_86249["CHEBI:86249"]
ing8_FeCl2_4H2O -.-> CHEBI_86249
ing9_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_007581 --> ing9_Yeast_extract__BD_Difco_
ing10_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
CultureMech_007581 --> ing10_Casamino_acids__BD_Difco_
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
distilled water
H2O 18.015 g/mol
|
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound Distilled water is used to bring the recipe to 1.0 L final volume. Its individual input volume and mass are unspecified; the imported 1 g/L is not supported, and the table's 1 L is not a fixed water dose. | |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound The modified recipe explicitly states 1.0 g/L MgSO4 heptahydrate (final), also reported as 1 g/L by TOGO M1064. Record this source-stated target, not 20 g/L copied from the base or an inferred 21 g/L sum. The word supplemented and the base recipe's 20 g per 1 L leave a preparation ambiguity: literal addition to the unchanged base cannot produce the stated lower target. This curation does not claim that operational ambiguity is resolved. |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
200 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component JCM168 specifies 200.0 g in the recipe brought to 1.0 L; 200.0 g / 1.0 L gives the stated per-liter rate. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". Hydrate form is retained; no molar or density conversion is used. JCM1007 inherits this base component; separate exact cross-reference: "Use Medium No. 168 supplemented with 1.0 g/L sodium pyruvate and 1.0 g/L MgSO4·7H2O (final).". |
| 4 |
KCl
Cl.K 74.551 g/mol
|
2 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component JCM168 specifies 2.0 g in the recipe brought to 1.0 L; 2.0 g / 1.0 L gives the stated per-liter rate. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". Hydrate form is retained; no molar or density conversion is used. JCM1007 inherits this base component; separate exact cross-reference: "Use Medium No. 168 supplemented with 1.0 g/L sodium pyruvate and 1.0 g/L MgSO4·7H2O (final).". |
| 5 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.36 MG_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component JCM168 specifies 0.36 mg in the recipe brought to 1.0 L; 0.36 mg / 1.0 L gives the stated per-liter rate. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". Hydrate form is retained; no molar or density conversion is used. JCM1007 inherits this base component; separate exact cross-reference: "Use Medium No. 168 supplemented with 1.0 g/L sodium pyruvate and 1.0 g/L MgSO4·7H2O (final).". |
| 6 |
sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
1 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component Explicit 1.0 g/L sodium pyruvate supplement in JCM1007; not present in unmodified JCM168. |
| 7 |
Sodium glutamate
C5H8NO4.Na 169.112 g/mol
|
1 G_PER_L |
CHEBI:64220
monosodium glutamate
|
Role: Nitrogen source; Properties: Defined component, Simple component, Organic compound JCM168 specifies 1.0 g in the recipe brought to 1.0 L; 1.0 g / 1.0 L gives the stated per-liter rate. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". Hydrate form is retained; no molar or density conversion is used. JCM1007 inherits this base component; separate exact cross-reference: "Use Medium No. 168 supplemented with 1.0 g/L sodium pyruvate and 1.0 g/L MgSO4·7H2O (final).". |
| 8 |
Trisodium citrate
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Defined component, Simple component, Organic compound JCM168 specifies 3.0 g in the recipe brought to 1.0 L; 3.0 g / 1.0 L gives the stated per-liter rate. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". Hydrate form is retained; no molar or density conversion is used. JCM1007 inherits this base component; separate exact cross-reference: "Use Medium No. 168 supplemented with 1.0 g/L sodium pyruvate and 1.0 g/L MgSO4·7H2O (final).". |
| 9 |
FeCl2・4H2O
Cl2Fe.4H2O 198.811 g/mol
|
36 MG_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component JCM168 specifies 36.0 mg in the recipe brought to 1.0 L; 36.0 mg / 1.0 L gives the stated per-liter rate. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". Hydrate form is retained; no molar or density conversion is used. JCM1007 inherits this base component; separate exact cross-reference: "Use Medium No. 168 supplemented with 1.0 g/L sodium pyruvate and 1.0 g/L MgSO4·7H2O (final).". |
| 10 | Yeast extract (BD-Difco) | 5 G_PER_L | Properties: Undefined component JCM168 specifies 5.0 g in the recipe brought to 1.0 L; 5.0 g / 1.0 L gives the stated per-liter rate. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". Hydrate form is retained; no molar or density conversion is used. JCM1007 inherits this base component; separate exact cross-reference: "Use Medium No. 168 supplemented with 1.0 g/L sodium pyruvate and 1.0 g/L MgSO4·7H2O (final).". | |
| 11 | Casamino acids (BD-Difco) | 5 G_PER_L | Properties: Undefined component JCM168 specifies 5.0 g in the recipe brought to 1.0 L; 5.0 g / 1.0 L gives the stated per-liter rate. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.". Hydrate form is retained; no molar or density conversion is used. JCM1007 inherits this base component; separate exact cross-reference: "Use Medium No. 168 supplemented with 1.0 g/L sodium pyruvate and 1.0 g/L MgSO4·7H2O (final).". |
Solutions
Na2CO3 solution
Dose: variable VARIABLE
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
Sterile Na2CO3 solution is added after autoclaving to adjust pH to 8.0. Neither a fixed dose nor a stock strength is supplied; no inline strength is invented.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.855558Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1064 |
| 2026-01-28T19:48:19.274719Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Na2CO3 solution |
| 2026-03-09T08:04:31.892478+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:49.835904Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007581 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:06.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:08.187614+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified Halobacteria Medium-2" to "modified_halobacteria_medium_2" for consistent matching |
| 2026-05-16T09:03:07.293857+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:30.126518+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:20.995904+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-10-09T12:51:15.611095+00:00 | codex | CURATED_HALOBACTERIA_SOURCE_CONCENTRATIONS | Source-specific ingredient review with exact PURL-backed excerpts. Trace metals use mg/L, water is final-volume make-up, and modified-medium magnesium retains the explicit source target and preparation ambiguity. Existing chemical mappings and high-metal flags are preserved; no taxa-media claims are added. |