clostridium_swellfunianum_medium

CultureMech:007583 bacterial COMPLEX LIQUID
Original name: Clostridium Swellfunianum Medium

Overview

External ontology
TOGO:M1066 — Clostridium Swellfunianum Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1066 Original source: JCM - JCM_M1009 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1009

Composition

flowchart LR
    CultureMech_007583["clostridium_swellfunianum_medium"]
    ing0_Sol__1["Sol. 1"]
    CultureMech_007583 --> ing0_Sol__1
    ing1_Sol__2["Sol. 2"]
    CultureMech_007583 --> ing1_Sol__2
    ing2_Distilled_water["Distilled water"]
    CultureMech_007583 --> ing2_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing2_Distilled_water -.-> CHEBI_15377
    ing3_KH2PO4["KH2PO4"]
    CultureMech_007583 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_NH4Cl["NH4Cl"]
    CultureMech_007583 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_KCl["KCl"]
    CultureMech_007583 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_007583 --> ing6_Yeast_extract__BD_Difco_
    ing7_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
    CultureMech_007583 --> ing7_Tryptone__BD_Difco_
    ing8_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
    CultureMech_007583 --> ing8_L__Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing8_L__Cysteine_HCl_H2O -.-> CHEBI_91248
    ing9_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_007583 --> ing9_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing9_Carbon_dioxide_gas -.-> CHEBI_16526
    ing10_Nitrogen_gas["Nitrogen gas"]
    CultureMech_007583 --> ing10_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing10_Nitrogen_gas -.-> CHEBI_17997

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Sol. 1 5 G_PER_L
2 Sol. 2 0.2 G_PER_L
3 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component
4 KH2PO4
H2O4P.K 136.084 g/mol
0.33 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
5 NH4Cl
Cl.H4N 53.492 g/mol
0.33 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component
6 KCl
Cl.K 74.551 g/mol
0.33 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
7 Yeast extract (BD-Difco) 0.5 G_PER_L Properties: Undefined component
8 Tryptone (BD-Difco) 3 G_PER_L Properties: Undefined component
9 L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
 Properties: Defined component, Simple component, Organic compound
10 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
11 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス

Solutions

1% CaCl2・2H2O solution

Dose: 33 G_PER_L

Role: Mineral source; Properties: Inorganic compound, Solution, Defined component

2% MgCl2・6H2O solution

Dose: 16.5 G_PER_L

Role: Mineral source; Properties: Defined component, Inorganic compound, Solution

1% Resazurin solution

Dose: 1 G_PER_L

Role: pH dependent redox indicator; Properties: Defined component, Solution

Trace element solution SL--10 (see Medium [M433])

Dose: 1 G_PER_L

Cross-reference to Medium M433

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

10% (w/v) NaHCO3 solution*

Dose: 0.05 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

20% (w/v) Glucose solution

Dose: 0.1 G_PER_L

Role: Carbon source; Properties: Organic compound, Solution, Defined component

3% (w/v) Na2S・9H2O solution

Dose: 0.05 G_PER_L

Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:53.858693Z togo-import Imported from TOGO Medium Source: JCM, ID: M1066
2026-01-28T19:48:07.226518Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:04:11.659235+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 8 solution(s) from ingredients
2026-03-13T06:19:49.850448Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007583
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:08.202377+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Clostridium Swellfunianum Medium" to "clostridium_swellfunianum_medium" for consistent matching
2026-05-16T09:03:07.307760+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:30.144250+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:46.827218+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:05.430904+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 98e251edbb0ec497c06388dabd0b475d6aeb5890d54228b3f570f0c630e6eb80