Overview
- External ontology
- TOGO:M1066 — Clostridium Swellfunianum Medium
- Applications
-
- Microbial cultivation
- pH
- 7.3
- Notes
- TOGO M1066 records JCM Medium 1009 with 5.0 ml Sol. 1 dispensed anaerobically under N2-CO2 (4:1, v/v), sealed and autoclaved, then supplemented after autoclaving with 0.2 ml Sol. 2 anaerobic stocks per 5.0 ml medium. Sol. 1 contains CaCl2, MgCl2, Resazurin, Trace element solution SL-10 from M433/JCM 433, Trace vitamins from M190/JCM 197, NH4Cl, KCl, KH2PO4, yeast extract, Tryptone, L-Cysteine HCl H2O, distilled water, and N2/CO2 gas; Sol. 2 supplies NaHCO3, Glucose, and Na2S x 9H2O stocks.
Ingredients (0)
No ingredients recorded.
Preparation
- Prepare Sol. 1 with salts, yeast extract, tryptone, cysteine, Trace element solution SL-10, Trace vitamins, CaCl2, MgCl2, and Resazurin stocks, and distilled water.
- Adjust the base medium to pH 7.3.
- Prepare the medium anaerobically under N2-CO2 (4:1, v/v), distribute 5.0 ml aliquots under the same gas mixture, seal, and autoclave.
- After autoclaving, add 0.05 ml 3% Na2S x 9H2O, 0.05 ml filter-sterilized 10% NaHCO3, and 0.1 ml 20% Glucose anaerobic stocks per 5.0 ml medium.
Solutions
Sol. 1
Dose: 5.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NH4Cl | 0.33 G_PER_L | TOGO M1066 / JCM Medium 1009 lists 0.33 g/L NH4Cl. |
| KCl | 0.33 G_PER_L | TOGO M1066 / JCM Medium 1009 lists 0.33 g/L KCl. |
| KH2PO4 | 0.33 G_PER_L | TOGO M1066 / JCM Medium 1009 lists 0.33 g/L KH2PO4. |
| Yeast extract (BD-Difco) | 0.5 G_PER_L | TOGO M1066 / JCM Medium 1009 lists 0.5 g/L Yeast extract (BD-Difco). |
| Tryptone (BD-Difco) | 3.0 G_PER_L | TOGO M1066 / JCM Medium 1009 lists 3.0 g/L Tryptone (BD-Difco). |
| Trace element solution SL-10 | 1.0 ML_PER_L | TOGO M1066 / JCM Medium 1009 adds 1.0 ml/L Trace element solution SL-10. |
| Trace vitamins | 10.0 ML_PER_L | TOGO M1066 / JCM Medium 1009 adds 10.0 ml/L Trace vitamins. |
| 1% CaCl2 x 2H2O solution | 33.0 ML_PER_L | TOGO M1066 / JCM Medium 1009 adds 33.0 ml/L 1% CaCl2 x 2H2O solution. |
| 2% MgCl2 x 6H2O solution | 16.5 ML_PER_L | TOGO M1066 / JCM Medium 1009 adds 16.5 ml/L 2% MgCl2 x 6H2O solution. |
| L-Cysteine HCl H2O | 0.5 G_PER_L | TOGO M1066 / JCM Medium 1009 lists 0.5 g/L L-Cysteine HCl H2O. |
| 1% Resazurin solution | 1.0 ML_PER_L | TOGO M1066 / JCM Medium 1009 adds 1.0 ml/L 1% Resazurin solution. |
| Distilled water | 1.0 L | TOGO M1066 / JCM Medium 1009 lists 1.0 L Distilled water. |
| N2 | variable VARIABLE | TOGO M1066 / JCM Medium 1009 prepares the medium under an N2-CO2 gas mixture. |
| CO2 | variable VARIABLE | TOGO M1066 / JCM Medium 1009 prepares the medium under an N2-CO2 (4:1, v/v) gas mixture. |
TOGO M1066 / JCM Medium 1009 prepares the base anaerobic medium and dispenses 5.0 ml portions before autoclaving.
Sol. 2
Dose: 0.2 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| 3% (w/v) Na2S x 9H2O solution | variable VARIABLE | TOGO M1066 / JCM Medium 1009 adds 0.05 ml 3% (w/v) Na2S x 9H2O solution per 5.0 ml medium after autoclaving. |
| 10% (w/v) NaHCO3 solution | variable VARIABLE | TOGO M1066 / JCM Medium 1009 adds 0.05 ml 10% (w/v) NaHCO3 solution per 5.0 ml medium after autoclaving. |
| 20% (w/v) Glucose solution | variable VARIABLE | TOGO M1066 / JCM Medium 1009 adds 0.1 ml 20% (w/v) Glucose solution per 5.0 ml medium after autoclaving. |
TOGO M1066 / JCM Medium 1009 adds 0.2 ml anaerobic stock solutions per 5.0 ml medium after autoclaving.
1% CaCl2 x 2H2O solution
Dose: 33.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| CaCl2 x 2H2O | 10.0 G_PER_L | TOGO M1066 / JCM Medium 1009 lists 1% CaCl2 x 2H2O solution, equivalent to 10.0 g/L CaCl2 x 2H2O. |
TOGO M1066 / JCM Medium 1009 references 1% CaCl2 x 2H2O solution; the listed percentage stock is represented as 10.0 g/L CaCl2 x 2H2O.
2% MgCl2 x 6H2O solution
Dose: 16.5 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MgCl2 x 6H2O | 20.0 G_PER_L | TOGO M1066 / JCM Medium 1009 lists 2% MgCl2 x 6H2O solution, equivalent to 20.0 g/L MgCl2 x 6H2O. |
TOGO M1066 / JCM Medium 1009 references 2% MgCl2 x 6H2O solution; the listed percentage stock is represented as 20.0 g/L MgCl2 x 6H2O.
1% Resazurin solution
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Resazurin | 10.0 G_PER_L | TOGO M1066 / JCM Medium 1009 lists 1% Resazurin solution, equivalent to 10.0 g/L Resazurin. |
TOGO M1066 / JCM Medium 1009 references 1% Resazurin solution; the listed percentage stock is represented as 10.0 g/L Resazurin.
Trace element solution SL-10
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| HCl (25%, 7.7 M) | 10.0 ML_PER_L | TOGO M433 / JCM Medium 433 lists 10.0 ml/L HCl (25%, 7.7 M). |
| FeCl2 x 4H2O | 1.5 G_PER_L | TOGO M433 / JCM Medium 433 lists 1.5 g/L FeCl2 x 4H2O. |
| ZnCl2 | 70.0 MG_PER_L | TOGO M433 / JCM Medium 433 lists 70.0 mg/L ZnCl2. |
| MnCl2 x 4H2O | 100.0 MG_PER_L | TOGO M433 / JCM Medium 433 lists 100.0 mg/L MnCl2 x 4H2O. |
| H3BO3 | 6.0 MG_PER_L | TOGO M433 / JCM Medium 433 lists 6.0 mg/L H3BO3. |
| CoCl2 x 6H2O | 190.0 MG_PER_L | TOGO M433 / JCM Medium 433 lists 190.0 mg/L CoCl2 x 6H2O. |
| CuCl2 x 2H2O | 2.0 MG_PER_L | TOGO M433 / JCM Medium 433 lists 2.0 mg/L CuCl2 x 2H2O. |
| NiCl2 x 6H2O | 24.0 MG_PER_L | TOGO M433 / JCM Medium 433 lists 24.0 mg/L NiCl2 x 6H2O. |
| Na2MoO4 x 2H2O | 36.0 MG_PER_L | TOGO M433 / JCM Medium 433 lists 36.0 mg/L Na2MoO4 x 2H2O. |
| Distilled water | 990.0 ML_PER_L | TOGO M433 / JCM Medium 433 lists 990.0 ml/L Distilled water. |
TOGO M1066 / JCM Medium 1009 adds 1.0 ml/L Trace element solution SL-10 from M433.
Trace vitamins
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 2.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 2.0 mg/L Biotin. |
| Folic acid | 2.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 2.0 mg/L Folic acid. |
| Pyridoxine HCl | 10.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 10.0 mg/L Pyridoxine HCl. |
| Thiamine HCl | 5.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 5.0 mg/L Thiamine HCl. |
| Riboflavin | 5.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 5.0 mg/L Riboflavin. |
| Nicotinic acid | 5.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 5.0 mg/L Nicotinic acid. |
| Calcium pantothenate | 5.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 5.0 mg/L Calcium pantothenate. |
| Vitamin B12 | 0.1 MG_PER_L | TOGO M190 / JCM Medium 197 lists 0.1 mg/L Vitamin B12. |
| p-Aminobenzoic acid | 5.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 5.0 mg/L p-Aminobenzoic acid. |
| Lipoic acid | 5.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 5.0 mg/L Lipoic acid. |
| Distilled water | 1.0 L | TOGO M190 / JCM Medium 197 lists 1.0 L Distilled water. |
TOGO M1066 / JCM Medium 1009 adds 10.0 ml/L Trace vitamins from M190.
10% (w/v) NaHCO3 solution
Dose: variable VARIABLE
| Ingredient | Concentration | Notes |
|---|---|---|
| NaHCO3 | 100.0 G_PER_L | TOGO M1066 / JCM Medium 1009 lists 10% (w/v) NaHCO3 solution, equivalent to 100.0 g/L NaHCO3. |
TOGO M1066 / JCM Medium 1009 references 10% (w/v) NaHCO3 solution; the listed percentage stock is represented as 100.0 g/L NaHCO3.
20% (w/v) Glucose solution
Dose: variable VARIABLE
| Ingredient | Concentration | Notes |
|---|---|---|
| Glucose | 200.0 G_PER_L | TOGO M1066 / JCM Medium 1009 lists 20% (w/v) Glucose solution, equivalent to 200.0 g/L Glucose. |
TOGO M1066 / JCM Medium 1009 references 20% (w/v) Glucose solution; the listed percentage stock is represented as 200.0 g/L Glucose.
3% (w/v) Na2S x 9H2O solution
Dose: variable VARIABLE
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2S x 9H2O | 30.0 G_PER_L | TOGO M1066 / JCM Medium 1009 lists 3% (w/v) Na2S x 9H2O solution, equivalent to 30.0 g/L Na2S x 9H2O. |
TOGO M1066 / JCM Medium 1009 references 3% (w/v) Na2S x 9H2O solution; the listed percentage stock is represented as 30.0 g/L Na2S x 9H2O.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.858693Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1066 |
| 2026-01-28T19:48:07.226518Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:11.659235+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 8 solution(s) from ingredients |
| 2026-03-13T06:19:49.850448Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007583 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:08.202377+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Clostridium Swellfunianum Medium" to "clostridium_swellfunianum_medium" for consistent matching |
| 2026-05-16T09:03:07.307760+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:30.144250+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:46.827218+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m1066_score15.py | RESOLVED_TOGO_M1066_SCORE15 | TOGO M1066 records JCM Medium 1009 with 5.0 ml Sol. 1 dispensed anaerobically under N2-CO2 (4:1, v/v), sealed and autoclaved, then supplemented after autoclaving with 0.2 ml Sol. 2 anaerobic stocks per 5.0 ml medium. Sol. 1 contains CaCl2, MgCl2, Resazurin, Trace element solution SL-10 from M433/JCM 433, Trace vitamins from M190/JCM 197, NH4Cl, KCl, KH2PO4, yeast extract, Tryptone, L-Cysteine HCl H2O, distilled water, and N2/CO2 gas; Sol. 2 supplies NaHCO3, Glucose, and Na2S x 9H2O stocks. |