Overview
- External ontology
- TOGO:M1162 — Natronolimnobius AHT32 Medium (B)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1162 Original source: JCM - JCM_M1092 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1092
Composition
flowchart LR
CultureMech_007686["natronolimnobius_aht32_medium_b"]
ing0_1_M_Sodium_acetate["1 M Sodium acetate"]
CultureMech_007686 --> ing0_1_M_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing0_1_M_Sodium_acetate -.-> CHEBI_32954
ing1_1_M_Sodium_butyrate["1 M Sodium butyrate"]
CultureMech_007686 --> ing1_1_M_Sodium_butyrate
CHEBI_64103["CHEBI:64103"]
ing1_1_M_Sodium_butyrate -.-> CHEBI_64103
ing2_Nitrogen_gas["Nitrogen gas"]
CultureMech_007686 --> ing2_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing2_Nitrogen_gas -.-> CHEBI_17997
ing3_Oxygen_gas["Oxygen gas"]
CultureMech_007686 --> ing3_Oxygen_gas
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
1 M Sodium acetate
C2H3O2.Na 82.034 g/mol
|
20 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Defined component, Solution, Organic compound |
| 2 |
1 M Sodium butyrate
C4H7O2.Na 110.088 g/mol
|
20 G_PER_L |
CHEBI:64103
sodium butyrate
|
Properties: Defined component, Organic compound, Solution |
| 3 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 4 | Oxygen gas | variable VARIABLE | Properties: ガス |
Solutions
Soda based mineral medium (see Medium [M1150])
Dose: 1 G_PER_L
Cross-reference to Medium M1150
10% Yeast extract solution
Dose: 0.2 G_PER_L
Role: Nutrient source; Properties: Undefined component
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1162_Natronolimnobius_AHT32_Medium_B (CultureMech:007686)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.036035Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1162 |
| 2026-01-28T19:48:12.050608Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Oxygen gas |
| 2026-03-09T08:04:19.877994+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T06:19:50.608964Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007686 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.008596+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Natronolimnobius AHT32 Medium (B)" to "natronolimnobius_aht32_medium_b" for consistent matching |
| 2026-05-16T09:03:08.044149+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:30.918012+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:04:24.672138+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 54519f0f2a8fb534c5a8989279b6b7aa9f9472b1589c078bc69887f4d6d65099 |