Overview
- External ontology
- TOGO:M1162 — Natronolimnobius AHT32 Medium (B)
- Applications
-
- Microbial cultivation
- Notes
- JCM Medium 1092 composes Natronolimnobius AHT32 Medium (B) from 1 L sterilized JCM 1081 Soda based mineral medium plus Trace vitamins, yeast extract, sodium butyrate, and sodium acetate stocks.
Composition
flowchart LR
CultureMech_007686["natronolimnobius_aht32_medium_b"]
ing0_N2["N2"]
CultureMech_007686 --> ing0_N2
CHEBI_17997["CHEBI:17997"]
ing0_N2 -.-> CHEBI_17997
ing1_O2["O2"]
CultureMech_007686 --> ing1_O2
CHEBI_15379["CHEBI:15379"]
ing1_O2 -.-> CHEBI_15379
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
JCM Medium 1092 replaces the gas phase with N2 containing 5% O2 by volume. |
| 2 |
O2
O2 31.998 g/mol
|
variable VARIABLE |
CHEBI:15379
dioxygen
|
JCM Medium 1092 replaces the gas phase with N2 containing 5% O2 by volume. |
Preparation
- Prepare 1.0 L of sterilized JCM 1081 Soda based mineral medium.
- Aseptically add Trace vitamins, yeast extract, sodium butyrate, and sodium acetate stocks.
- Distribute into culture vessels, replace the gas phase with N2 containing 5% O2, and seal with butyl rubber stoppers.
Solutions
Soda based mineral medium
Dose: 1000.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2CO3 | 185.0 G_PER_L | JCM Medium 1081 prints this component in its stock solution. |
| NaHCO3 | 35.0 G_PER_L | JCM Medium 1081 prints this component in its stock solution. |
| NaCl | 16.0 G_PER_L | JCM Medium 1081 prints this component in its stock solution. |
| K2HPO4 | 1.0 G_PER_L | JCM Medium 1081 prints this component in its stock solution. |
| KCl | 5.0 G_PER_L | JCM Medium 1081 prints this component in its stock solution. |
| 4 M NH4Cl solution | 1.0 ML_PER_L | JCM Medium 1081 adds 1.0 ml/L 4 M NH4Cl solution to the soda based mineral medium. |
| Selenite-tungstate solution | 1.0 ML_PER_L | JCM Medium 1081 adds 1.0 ml/L JCM 431 Selenite-tungstate solution. |
| Trace element solution | 1.0 ML_PER_L | JCM Medium 1081 adds 1.0 ml/L JCM 1079 Trace element solution. |
| 1 M MgCl2 solution | 1.0 ML_PER_L | JCM Medium 1081 adds 1.0 ml/L 1 M MgCl2 solution to the soda based mineral medium. |
| Distilled water | 1.0 L | JCM Medium 1081 prints this component in its stock solution. |
JCM Medium 1092 adds 1000.0 ml/L sterilized Soda based mineral medium from JCM Medium 1081.
Trace vitamins
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 2.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Folic acid | 2.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Pyridoxine HCl | 10.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Thiamine HCl | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Riboflavin | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Nicotinic acid | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Calcium pantothenate | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Vitamin B12 | 0.1 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| p-Aminobenzoic acid | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Lipoic acid | 5.0 MG_PER_L | JCM Medium 197 prints this component in its stock solution. |
| Distilled water | 1.0 L | JCM Medium 197 prints this component in its stock solution. |
JCM Medium 1092 adds 10.0 ml/L Trace vitamins from JCM Medium 197.
10% Yeast extract solution
Dose: 0.2 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Yeast extract | 10 PERCENT_W_V | JCM Medium 1092 identifies this as a 10% w/v stock solution. |
JCM Medium 1092 adds 0.2 ml/L 10% Yeast extract solution.
1 M Sodium butyrate
Dose: 20.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Sodium butyrate | 1.0 MOLAR | JCM Medium 1092 identifies this as a 1.0 M stock solution. |
JCM Medium 1092 adds 20.0 ml/L 1 M Sodium butyrate.
1 M Sodium acetate
Dose: 20.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Sodium acetate | 1.0 MOLAR | JCM Medium 1092 identifies this as a 1.0 M stock solution. |
JCM Medium 1092 adds 20.0 ml/L 1 M Sodium acetate.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.036035Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1162 |
| 2026-01-28T19:48:12.050608Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Oxygen gas |
| 2026-03-09T08:04:19.877994+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T06:19:50.608964Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007686 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.008596+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Natronolimnobius AHT32 Medium (B)" to "natronolimnobius_aht32_medium_b" for consistent matching |
| 2026-05-16T09:03:08.044149+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:30.918012+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-10T00:00:00-07:00 | repair_togo_jcm_archaea_score15.py | RESOLVED_TOGO_JCM_ARCHAEA_SCORE15 | JCM Medium 1092 composes Natronolimnobius AHT32 Medium (B) from 1 L sterilized JCM 1081 Soda based mineral medium plus Trace vitamins, yeast extract, sodium butyrate, and sodium acetate stocks. |