seawater_pyruvate_thiosulfate_medium

CultureMech:007695 bacterial COMPLEX LIQUID
Original name: Seawater Pyruvate Thiosulfate Medium

Overview

External ontology
TOGO:M1170 — Seawater Pyruvate Thiosulfate Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1170 Original source: JCM - JCM_M1097 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1097

Composition

flowchart LR
    CultureMech_007695["seawater_pyruvate_thiosulfate_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007695 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_007695 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_007695 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_007695 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_NH4Cl["NH4Cl"]
    CultureMech_007695 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_Resazurin["Resazurin"]
    CultureMech_007695 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_007695 --> ing6_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_6H2O -.-> CHEBI_86345
    ing7_KCl["KCl"]
    CultureMech_007695 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_007695 --> ing8_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing8_Carbon_dioxide_gas -.-> CHEBI_16526
    ing9_Nitrogen_gas["Nitrogen gas"]
    CultureMech_007695 --> ing9_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing9_Nitrogen_gas -.-> CHEBI_17997
    ing10_Biotin["Biotin"]
    CultureMech_007695 --> ing10_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing10_Biotin -.-> CHEBI_15956
    ing11_p__Aminobenzoic_acid["p--Aminobenzoic acid"]
    CultureMech_007695 --> ing11_p__Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing11_p__Aminobenzoic_acid -.-> CHEBI_30753
    ing12_Thiamine_HCl["Thiamine・HCl"]
    CultureMech_007695 --> ing12_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing12_Thiamine_HCl -.-> CHEBI_49105
    ing13_Pyridoxine_HCl["Pyridoxine・HCl"]
    CultureMech_007695 --> ing13_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing13_Pyridoxine_HCl -.-> CHEBI_30961
    ing14_Folic_acid["Folic acid"]
    CultureMech_007695 --> ing14_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing14_Folic_acid -.-> CHEBI_27470
    ing15_Riboflavin["Riboflavin"]
    CultureMech_007695 --> ing15_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing15_Riboflavin -.-> CHEBI_17015
    ing16_Nicotinic_acid["Nicotinic acid"]
    CultureMech_007695 --> ing16_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing16_Nicotinic_acid -.-> CHEBI_15940
    ing17_Coenzyme_M["Coenzyme M"]
    CultureMech_007695 --> ing17_Coenzyme_M
    CHEBI_17905["CHEBI:17905"]
    ing17_Coenzyme_M -.-> CHEBI_17905
    ing18_Lipoic_acid["Lipoic acid"]
    CultureMech_007695 --> ing18_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing18_Lipoic_acid -.-> CHEBI_16494
    ing19_DL__Calcium_pantothenate["DL--Calcium pantothenate"]
    CultureMech_007695 --> ing19_DL__Calcium_pantothenate

Ingredients (20)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
923.5 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 922.5, 1.0]
2 NaCl
Cl.Na 58.443 g/mol
20 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.15 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4 KH2PO4
H2O4P.K 136.084 g/mol
0.2 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
5 NH4Cl
Cl.H4N 53.492 g/mol
0.25 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
6 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component
7 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
3 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
8 KCl
Cl.K 74.551 g/mol
0.5 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
9 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
10 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
11 Biotin
C10H16N2O3S 244.316 g/mol
2 G_PER_L CHEBI:15956
biotin
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
12 p--Aminobenzoic acid
C7H7NO2 137.138 g/mol
5 G_PER_L CHEBI:30753
4-aminobenzoic acid
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
13 Thiamine・HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
5 G_PER_L CHEBI:49105
thiamine hydrochloride
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
14 Pyridoxine・HCl
C8H11NO3.HCl 205.641 g/mol
10 G_PER_L CHEBI:30961
pyridoxine hydrochloride
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
15 Folic acid
C19H19N7O6 441.404 g/mol
2 G_PER_L CHEBI:27470
folic acid
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
16 Riboflavin
C17H20N4O6 376.369 g/mol
5 G_PER_L CHEBI:17015
riboflavin
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
17 Nicotinic acid
C6H5NO2 123.111 g/mol
5 G_PER_L CHEBI:15940
nicotinic acid
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
18 Coenzyme M
C2H6O3S2 142.201 g/mol
5 G_PER_L CHEBI:17905
coenzyme M
 Role: Growth factor; Properties: Organic compound, Defined component, Simple component
19 Lipoic acid
C8H14O2S2 206.332 g/mol
5 G_PER_L CHEBI:16494
lipoic acid
 Role: Growth factor; Properties: Organic compound, Defined component, Simple component
20 DL--Calcium pantothenate 5 G_PER_L Role: Vitamin; Properties: Organic compound, Defined component, Simple component

Solutions

RST trace elements (see Medium [M979])

Dose: 10 G_PER_L

Cross-reference to Medium M979

8% NaHCO3 solution*

Dose: 37.5 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

1% Yeast extract solution

Dose: 10 G_PER_L

Role: Nutrient source; Properties: Undefined component

1 M Sodium pyruvate solution

Dose: 20 G_PER_L

Role: Carbon source; Properties: Solution, Defined component, Organic compound

RST vitamin solution* (see below)

Dose: 10 G_PER_L

Reducing agent solution (see Medium [M522])

Dose: 10 G_PER_L

Cross-reference to Medium M522

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:54.051244Z togo-import Imported from TOGO Medium Source: JCM, ID: M1170
2026-01-28T19:48:26.396094Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:04:43.822389+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 6 solution(s) from ingredients
2026-03-13T03:09:13.996969Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:19:50.682776Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007695
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:21.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:09.085508+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Seawater Pyruvate Thiosulfate Medium" to "seawater_pyruvate_thiosulfate_medium" for consistent matching
2026-05-16T09:03:08.120363+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:31.000299+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:29.950414+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 1 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:47.105189+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:06.296873+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 99d004cdfd27a371f1b2db1b84330c4b95f0ae033b9888895078a8631621da0c