Overview
- External ontology
- TOGO:M1170 — Seawater Pyruvate Thiosulfate Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1170 Original source: JCM - JCM_M1097 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1097
Composition
flowchart LR
CultureMech_007695["seawater_pyruvate_thiosulfate_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007695 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_007695 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007695 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_007695 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_007695 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_Resazurin["Resazurin"]
CultureMech_007695 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_007695 --> ing6_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_6H2O -.-> CHEBI_86345
ing7_KCl["KCl"]
CultureMech_007695 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007695 --> ing8_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing8_Carbon_dioxide_gas -.-> CHEBI_16526
ing9_Nitrogen_gas["Nitrogen gas"]
CultureMech_007695 --> ing9_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing9_Nitrogen_gas -.-> CHEBI_17997
ing10_Biotin["Biotin"]
CultureMech_007695 --> ing10_Biotin
CHEBI_15956["CHEBI:15956"]
ing10_Biotin -.-> CHEBI_15956
ing11_p__Aminobenzoic_acid["p--Aminobenzoic acid"]
CultureMech_007695 --> ing11_p__Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing11_p__Aminobenzoic_acid -.-> CHEBI_30753
ing12_Thiamine_HCl["Thiamine・HCl"]
CultureMech_007695 --> ing12_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing12_Thiamine_HCl -.-> CHEBI_49105
ing13_Pyridoxine_HCl["Pyridoxine・HCl"]
CultureMech_007695 --> ing13_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing13_Pyridoxine_HCl -.-> CHEBI_30961
ing14_Folic_acid["Folic acid"]
CultureMech_007695 --> ing14_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing14_Folic_acid -.-> CHEBI_27470
ing15_Riboflavin["Riboflavin"]
CultureMech_007695 --> ing15_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing15_Riboflavin -.-> CHEBI_17015
ing16_Nicotinic_acid["Nicotinic acid"]
CultureMech_007695 --> ing16_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing16_Nicotinic_acid -.-> CHEBI_15940
ing17_Coenzyme_M["Coenzyme M"]
CultureMech_007695 --> ing17_Coenzyme_M
CHEBI_17905["CHEBI:17905"]
ing17_Coenzyme_M -.-> CHEBI_17905
ing18_Lipoic_acid["Lipoic acid"]
CultureMech_007695 --> ing18_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing18_Lipoic_acid -.-> CHEBI_16494
ing19_DL__Calcium_pantothenate["DL--Calcium pantothenate"]
CultureMech_007695 --> ing19_DL__Calcium_pantothenate
Ingredients (20)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
923.5 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 922.5, 1.0] |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
20 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 7 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
3 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 10 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 11 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 12 |
p--Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 13 |
Thiamine・HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
5 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 14 |
Pyridoxine・HCl
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 15 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 16 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 17 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 18 |
Coenzyme M
C2H6O3S2 142.201 g/mol
|
5 G_PER_L |
CHEBI:17905
coenzyme M
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 19 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
5 G_PER_L |
CHEBI:16494
lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 20 | DL--Calcium pantothenate | 5 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
Solutions
RST trace elements (see Medium [M979])
Dose: 10 G_PER_L
Cross-reference to Medium M979
8% NaHCO3 solution*
Dose: 37.5 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
1% Yeast extract solution
Dose: 10 G_PER_L
Role: Nutrient source; Properties: Undefined component
1 M Sodium pyruvate solution
Dose: 20 G_PER_L
Role: Carbon source; Properties: Solution, Defined component, Organic compound
RST vitamin solution* (see below)
Dose: 10 G_PER_L
Reducing agent solution (see Medium [M522])
Dose: 10 G_PER_L
Cross-reference to Medium M522
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.051244Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1170 |
| 2026-01-28T19:48:26.396094Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:43.822389+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T03:09:13.996969Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:50.682776Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007695 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:21.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.085508+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Seawater Pyruvate Thiosulfate Medium" to "seawater_pyruvate_thiosulfate_medium" for consistent matching |
| 2026-05-16T09:03:08.120363+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:31.000299+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:29.950414+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 1 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:47.105189+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |