Overview
- External ontology
- TOGO:M1176 — Anaerobic Natural Seawater Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1176 Original source: JCM - JCM_M1102-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1102
Composition
flowchart LR
CultureMech_007701["anaerobic_natural_seawater_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007701 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_007701 --> ing1_Yeast_extract
ing2_Resazurin["Resazurin"]
CultureMech_007701 --> ing2_Resazurin
CHEBI_8806["CHEBI:8806"]
ing2_Resazurin -.-> CHEBI_8806
ing3_Natural_seawater__filtrated_["Natural seawater (filtrated)"]
CultureMech_007701 --> ing3_Natural_seawater__filtrated_
ing4_Tryptone["Tryptone"]
CultureMech_007701 --> ing4_Tryptone
ing5_N2["N2"]
CultureMech_007701 --> ing5_N2
CHEBI_17997["CHEBI:17997"]
ing5_N2 -.-> CHEBI_17997
ing6_sodium_pyruvate["sodium pyruvate"]
CultureMech_007701 --> ing6_sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing6_sodium_pyruvate -.-> CHEBI_50144
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
250 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Yeast extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Simple component, Defined component, Organic compound |
| 4 | Natural seawater (filtrated) | 750 G_PER_L | Role: Solvating media; Properties: Undefined component | |
| 5 | Tryptone | 5 G_PER_L | Properties: Undefined component | |
| 6 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 7 |
sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
variable VARIABLE |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Defined component, Organic compound, Solution |
Solutions
5% Na2S・9H2O solution
Dose: 6 G_PER_L
Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution
5% L-Cysteine・HCl・H2O solution
Dose: 6 G_PER_L
Properties: Defined component, Organic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1176_Anaerobic_Natural_Seawater_Medium (CultureMech:007701)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.068832Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1176 |
| 2026-01-28T19:48:23.527194Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2; Added default concentration for ingredient: sodium pyruvate |
| 2026-03-09T08:04:39.113877+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:19:50.749481Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007701 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:15.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.153453+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Anaerobic Natural Seawater Medium" to "anaerobic_natural_seawater_medium" for consistent matching |
| 2026-05-16T09:03:08.187984+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:31.077046+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:06.379162+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint dd7f2b5ddcc57942c0813eca28b90c6394b4078623fb082fefa071b98f0fe3b9 |