anaerobic_natural_seawater_medium (CultureMech:007701)

Overview

External ontology

TOGO:M1176 — Anaerobic Natural Seawater Medium

Applications

Microbial cultivation

pH

7.0

Notes

TOGO M1176 records JCM Medium 1102 as an anaerobic natural seawater base containing tryptone, yeast extract, resazurin, filtered natural seawater, distilled water, and an N2 atmosphere; the source adjusts the base to pH 7.0. After autoclaving and cooling, the source adds 6.0 ml/L each 5% L-Cysteine HCl H2O and 5% Na2S x 9H2O solutions. For strain JCM 32399, the source supplements 10 mM sodium pyruvate. JCM 32399 variant supplemented with 10 mM sodium pyruvate.

Composition

flowchart LR
    CultureMech_007701["anaerobic_natural_seawater_medium"]
    ing0_Tryptone["Tryptone"]
    CultureMech_007701 --> ing0_Tryptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_007701 --> ing1_Yeast_extract
    ing2_Resazurin["Resazurin"]
    CultureMech_007701 --> ing2_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing2_Resazurin -.-> CHEBI_8806
    ing3_Natural_seawater__filtrated_["Natural seawater (filtrated)"]
    CultureMech_007701 --> ing3_Natural_seawater__filtrated_
    ing4_Distilled_water["Distilled water"]
    CultureMech_007701 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377
    ing5_N2["N2"]
    CultureMech_007701 --> ing5_N2
    CHEBI_17997["CHEBI:17997"]
    ing5_N2 -.-> CHEBI_17997
    ing6_sodium_pyruvate["sodium pyruvate"]
    CultureMech_007701 --> ing6_sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing6_sodium_pyruvate -.-> CHEBI_50144

Ingredients (7)

#	Ingredient	Concentration	Ontology	Notes
1	Tryptone	5.0 G_PER_L		JCM Medium 1102 lists 5.0 g/L Tryptone.
2	Yeast extract	1.0 G_PER_L		JCM Medium 1102 lists 1.0 g/L Yeast extract.
3	Resazurin C12H7NO4 229.191 g/mol	0.5 MG_PER_L	CHEBI:8806 Resazurin	JCM Medium 1102 lists 0.5 mg/L Resazurin.
4	Natural seawater (filtrated)	750.0 ML_PER_L		JCM Medium 1102 lists 750.0 ml/L Natural seawater (filtrated).
5	Distilled water H2O 18.015 g/mol	250.0 ML_PER_L	CHEBI:15377 water	JCM Medium 1102 lists 250.0 ml/L Distilled water.
6	N2 N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	JCM Medium 1102 cools the boiled base, distributes the medium, and stores reducing stocks under an N2 gas atmosphere.
7	sodium pyruvate C3H3O3.Na 110.044 g/mol	10.0 MILLIMOLAR	CHEBI:50144 sodium pyruvate	JCM Medium 1102 supplements strain JCM 32399 with 10 mM sodium pyruvate.

Preparation

Mix tryptone, yeast extract, resazurin, natural seawater, and distilled water.
Adjust to pH 7.0.
Bring the medium to a boil and cool under an N2 gas atmosphere.
Distribute the medium in culture vessels under N2 and seal with butyl rubber stoppers.
Autoclave at 121 degrees C for 15 min.
After cooling, aseptically and anaerobically add 6.0 ml/L each 5% L-Cysteine HCl H2O and 5% Na2S x 9H2O solutions autoclaved and stored under N2.
Supplement the JCM 32399 variant with 10 mM sodium pyruvate.

Solutions

5% L-Cysteine HCl H2O solution

Dose: 6.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
L-Cysteine HCl H2O	5.0 PERCENT_W_V	5% L-Cysteine HCl H2O solution is represented from the stock label as 5.0% w/v L-Cysteine HCl H2O.

JCM Medium 1102 adds 6.0 ml/L 5% L-Cysteine HCl H2O solution.

5% Na2S x 9H2O solution

Dose: 6.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Na2S x 9H2O	5.0 PERCENT_W_V	5% Na2S x 9H2O solution is represented from the stock label as 5.0% w/v Na2S x 9H2O.

JCM Medium 1102 adds 6.0 ml/L 5% Na2S x 9H2O solution.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:54.068832Z	togo-import	Imported from TOGO Medium	Source: JCM, ID: M1176
2026-01-28T19:48:23.527194Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: N2; Added default concentration for ingredient: sodium pyruvate
2026-03-09T08:04:39.113877+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 2 solution(s) from ingredients
2026-03-13T06:19:50.749481Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:007701
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name; Added default solution name
2026-03-17T06:30:15.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:09.153453+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Anaerobic Natural Seawater Medium" to "anaerobic_natural_seawater_medium" for consistent matching
2026-05-16T09:03:08.187984+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:40:31.077046+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00	repair_togo_m1176_m1178_score15.py	RESOLVED_TOGO_M1176_M1178_SCORE15	TOGO M1176 records JCM Medium 1102 as an anaerobic natural seawater base containing tryptone, yeast extract, resazurin, filtered natural seawater, distilled water, and an N2 atmosphere; the source adjusts the base to pH 7.0. After autoclaving and cooling, the source adds 6.0 ml/L each 5% L-Cysteine HCl H2O and 5% Na2S x 9H2O solutions. For strain JCM 32399, the source supplements 10 mM sodium pyruvate. JCM 32399 variant supplemented with 10 mM sodium pyruvate.