Overview
- External ontology
- TOGO:M1203 — Artficial Freshwater Medium II
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1203 Original source: JCM - JCM_M1125 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1125
Composition
flowchart LR
CultureMech_007730["artficial_freshwater_medium_ii"]
ing0_Distilled_water["Distilled water"]
CultureMech_007730 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_KH2PO4["KH2PO4"]
CultureMech_007730 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_007730 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_Resazurin["Resazurin"]
CultureMech_007730 --> ing3_Resazurin
CHEBI_8806["CHEBI:8806"]
ing3_Resazurin -.-> CHEBI_8806
ing4_KCl["KCl"]
CultureMech_007730 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_Na2SO4["Na2SO4"]
CultureMech_007730 --> ing5_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing5_Na2SO4 -.-> CHEBI_32149
ing6_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007730 --> ing6_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing6_Carbon_dioxide_gas -.-> CHEBI_16526
ing7_Nitrogen_gas["Nitrogen gas"]
CultureMech_007730 --> ing7_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing7_Nitrogen_gas -.-> CHEBI_17997
ing8_N2["N2"]
CultureMech_007730 --> ing8_N2
ing8_N2 -.-> CHEBI_17997
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
960 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Simple component, Organic compound |
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.3 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 6 |
Na2SO4
2Na.O4S 142.043 g/mol
|
1.4 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 7 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 8 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 9 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Solutions
FeCl2 solution (see Medium [M180])
Dose: 1 G_PER_L
Cross-reference to Medium M180
Trace element solution (see Medium [M180])
Dose: 1 G_PER_L
Cross-reference to Medium M180
Selenite--tungstate solution (see Medium [M431])
Dose: 1 G_PER_L
Cross-reference to Medium M431
8% NaHCO3 solution*
Dose: 30 G_PER_L
Role: Buffer; Properties: Solution, Inorganic compound, Defined component
1 M CaCl2・2H2O solution
Dose: 1 G_PER_L
Role: Mineral source; Properties: Inorganic compound, Solution, Defined component
1 M MgCl2・6H2O solution
Dose: 2.5 G_PER_L
Role: Mineral source; Properties: Inorganic compound, Solution, Defined component
10% Yeast extract solution
Dose: 1 G_PER_L
Role: Nutrient source; Properties: Undefined component
1 M Glycerin solution*
Dose: 10 G_PER_L
Role: Carbon source; Properties: Defined component, Solution, Organic compound
Vitamin solution (see Medium [M401])
Dose: 1 G_PER_L
Cross-reference to Medium M401
Thiamine solution (see Medium [M401])
Dose: 1 G_PER_L
Cross-reference to Medium M401
Vitamin B12 solution (see Medium [M401])
Dose: 1 G_PER_L
Cross-reference to Medium M401
5% Na2S・9H2O solution
Dose: 5 G_PER_L
Role: Reducing agent; Properties: Solution, Inorganic compound, Defined component
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- TOGO_M1203_Artficial_Freshwater_Medium_II (CultureMech:007730)
- TOGO_M1204_Artficial_Freshwater_Medium_II (CultureMech:007731)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.121632Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1203 |
| 2026-01-28T19:48:15.426138Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:25.485512+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 12 solution(s) from ingredients |
| 2026-03-13T06:19:50.981512Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007730 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.390459+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Artficial Freshwater Medium II" to "artficial_freshwater_medium_ii" for consistent matching |
| 2026-05-16T09:03:08.393690+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:31.282863+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.200512+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:06.670763+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: TOGO_M1203_Artficial_Freshwater_Medium_II, TOGO_M1204_Artficial_Freshwater_Medium_II |
| 2026-08-06T03:05:06.670809+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint ca49c09f9c5d27624f291b273a36c64c479bb5a20bf23f8012c1d0d083f88dd6 |