Overview
- External ontology
- TOGO:M1203 — Artficial Freshwater Medium II
- Applications
-
- Microbial cultivation
- Notes
- TOGO M1203 records JCM Medium 1125 as Artficial Freshwater Medium II. JCM Medium 1125 / MediaDive J1125 supplies the complete base solution; JCM Media 187, 403, and 431 supply the FeCl2, Trace element, Selenite-tungstate, Vitamin, Thiamine, and Vitamin B12 stock compositions.
Composition
flowchart LR
CultureMech_007730["artficial_freshwater_medium_ii"]
ing0_KH2PO4["KH2PO4"]
CultureMech_007730 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_NH4Cl["NH4Cl"]
CultureMech_007730 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_KCl["KCl"]
CultureMech_007730 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_Na2SO4["Na2SO4"]
CultureMech_007730 --> ing3_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing3_Na2SO4 -.-> CHEBI_32149
ing4_Resazurin["Resazurin"]
CultureMech_007730 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_Distilled_water["Distilled water"]
CultureMech_007730 --> ing5_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing5_Distilled_water -.-> CHEBI_15377
ing6_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007730 --> ing6_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing6_Carbon_dioxide_gas -.-> CHEBI_16526
ing7_N2["N2"]
CultureMech_007730 --> ing7_N2
CHEBI_17997["CHEBI:17997"]
ing7_N2 -.-> CHEBI_17997
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.19685 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
JCM Medium 1125 lists KH2PO4 in the main solution; MediaDive J1125 normalizes this to 0.19685 g/L. |
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.246063 G_PER_L |
CHEBI:31206
ammonium chloride
|
JCM Medium 1125 lists NH4Cl in the main solution; MediaDive J1125 normalizes this to 0.246063 g/L. |
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.295276 G_PER_L |
CHEBI:32588
potassium chloride
|
JCM Medium 1125 lists KCl in the main solution; MediaDive J1125 normalizes this to 0.295276 g/L. |
| 4 |
Na2SO4
2Na.O4S 142.043 g/mol
|
1.37795 G_PER_L |
CHEBI:32149
sodium sulfate
|
JCM Medium 1125 lists Na2SO4 in the main solution; MediaDive J1125 normalizes this to 1.37795 g/L. |
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.984252 MG_PER_L |
CHEBI:8806
Resazurin
|
JCM Medium 1125 lists 1.0 mg Resazurin; MediaDive J1125 normalizes this to 0.984252 mg/L. |
| 6 |
Distilled water
H2O 18.015 g/mol
|
960.0 ML_PER_L |
CHEBI:15377
water
|
JCM Medium 1125 lists 960.0 ml distilled water before stock additions. |
| 7 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
JCM Medium 1125 uses an N2-CO2 (4:1, v/v) gas atmosphere. |
| 8 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
JCM Medium 1125 uses an N2-CO2 (4:1, v/v) gas atmosphere. |
Preparation
- Mix the base salts, FeCl2 solution, Trace element solution, Selenite-tungstate solution, resazurin, and distilled water.
- Autoclave the base medium under an N2-CO2 (4:1, v/v) gas atmosphere.
- After cooling, add the listed NaHCO3, MgCl2, CaCl2, vitamin, thiamine, vitamin B12, and yeast extract and glycerin stock solutions.
- Aseptically and anaerobically distribute under the same gas mixture and seal with butyl rubber stoppers.
- Prior to use, add 5.0 ml/L 5% Na2S x 9H2O solution.
Solutions
FeCl2 solution
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| HCl | 10.0 ML_PER_L | JCM Medium 187 lists 10.0 ml/L HCl. |
| FeCl2 x 4H2O | 1.5 G_PER_L | JCM Medium 187 lists 1.5 g/L FeCl2 x 4H2O. |
| Distilled water | 990.0 ML_PER_L | JCM Medium 187 lists 990.0 ml/L Distilled water. |
JCM Medium 1125 adds 1.0 ml/L FeCl2 solution from JCM Medium 187.
Trace element solution
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| ZnCl2 | 70.0 MG_PER_L | JCM Medium 187 lists 70.0 mg/L ZnCl2. |
| MnCl2 x 4H2O | 100.0 MG_PER_L | JCM Medium 187 lists 100.0 mg/L MnCl2 x 4H2O. |
| H3BO3 | 6.0 MG_PER_L | JCM Medium 187 lists 6.0 mg/L H3BO3. |
| CoCl2 x 6H2O | 190.0 MG_PER_L | JCM Medium 187 lists 190.0 mg/L CoCl2 x 6H2O. |
| CuCl2 x 2H2O | 2.0 MG_PER_L | JCM Medium 187 lists 2.0 mg/L CuCl2 x 2H2O. |
| NiCl2 x 6H2O | 24.0 MG_PER_L | JCM Medium 187 lists 24.0 mg/L NiCl2 x 6H2O. |
| Na2MoO4 x 2H2O | 36.0 MG_PER_L | JCM Medium 187 lists 36.0 mg/L Na2MoO4 x 2H2O. |
| Distilled water | 1.0 L | JCM Medium 187 lists 1.0 L Distilled water. |
JCM Medium 1125 adds 1.0 ml/L Trace element solution from JCM Medium 187.
Selenite-tungstate solution
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaOH | 0.4 G_PER_L | JCM Medium 431 lists 0.4 g/L NaOH. |
| Na2SeO3 x 5H2O | 6.0 MG_PER_L | JCM Medium 431 lists 6.0 mg/L Na2SeO3 x 5H2O. |
| Na2WO4 x 2H2O | 8.0 MG_PER_L | JCM Medium 431 lists 8.0 mg/L Na2WO4 x 2H2O. |
| Distilled water | 1.0 L | JCM Medium 431 lists 1.0 L Distilled water. |
JCM Medium 1125 adds 1.0 ml/L Selenite-tungstate solution from JCM Medium 431.
8% NaHCO3 solution
Dose: 30.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaHCO3 | 80.0 G_PER_L | JCM Medium 1125 lists 8% NaHCO3 solution; this records the stock as 80.0 g/L NaHCO3. |
JCM Medium 1125 adds 30.0 ml/L 8% NaHCO3 solution.
1 M MgCl2 x 6H2O solution
Dose: 2.5 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MgCl2 x 6H2O | 1.0 MOLAR | JCM Medium 1125 lists 1.0 M MgCl2 x 6H2O. |
JCM Medium 1125 adds 2.5 ml/L 1 M MgCl2 x 6H2O solution.
1 M CaCl2 x 2H2O solution
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| CaCl2 x 2H2O | 1.0 MOLAR | JCM Medium 1125 lists 1.0 M CaCl2 x 2H2O. |
JCM Medium 1125 adds 1.0 ml/L 1 M CaCl2 x 2H2O solution.
Vitamin solution
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| p-Aminobenzoic acid | 40.0 MG_PER_L | JCM Medium 403 lists 4.0 mg p-Aminobenzoic acid in 100.0 ml Vitamin solution; this records the stock as 40.0 mg/L. |
| Biotin | 10.0 MG_PER_L | JCM Medium 403 lists 1.0 mg Biotin in 100.0 ml Vitamin solution; this records the stock as 10.0 mg/L. |
| Nicotinic acid | 100.0 MG_PER_L | JCM Medium 403 lists 10.0 mg Nicotinic acid in 100.0 ml Vitamin solution; this records the stock as 100.0 mg/L. |
| Calcium pantothenate | 50.0 MG_PER_L | JCM Medium 403 lists 5.0 mg DL-Calcium pantothenate in 100.0 ml Vitamin solution; this records the stock as 50.0 mg/L. |
| Pyridoxine HCl | 100.0 MG_PER_L | JCM Medium 403 lists 10.0 mg Pyridoxine HCl in 100.0 ml Vitamin solution; this records the stock as 100.0 mg/L. |
| Sodium phosphate buffer (10 mM, pH 7.1) | 1000.0 ML_PER_L | JCM Medium 403 lists 100.0 ml Sodium phosphate buffer (10 mM, pH 7.1) as the 100.0 ml Vitamin solution base. |
JCM Medium 1125 adds 1.0 ml/L filter-sterilized Vitamin solution.
Thiamine solution
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Thiamine HCl | 100.0 MG_PER_L | JCM Medium 403 lists 10.0 mg Thiamine HCl in 100.0 ml Thiamine solution; this records the stock as 100.0 mg/L. |
| Sodium phosphate buffer (25 mM, pH 3.4) | 1000.0 ML_PER_L | JCM Medium 403 lists 100.0 ml Sodium phosphate buffer (25 mM, pH 3.4) as the 100.0 ml Thiamine solution base. |
JCM Medium 1125 adds 1.0 ml/L filter-sterilized Thiamine solution.
Vitamin B12 solution
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Vitamin B12 | 50.0 MG_PER_L | JCM Medium 403 lists 5.0 mg Vitamin B12 in 100.0 ml Vitamin B12 solution; this records the stock as 50.0 mg/L. |
| Distilled water | 1000.0 ML_PER_L | JCM Medium 403 lists 100.0 ml Distilled water as the 100.0 ml Vitamin B12 solution base. |
JCM Medium 1125 adds 1.0 ml/L filter-sterilized Vitamin B12 solution.
10% Yeast extract solution
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Yeast extract | 100.0 G_PER_L | JCM Medium 1125 lists 10% Yeast extract solution; this records the stock as 100.0 g/L Yeast extract. |
JCM Medium 1125 adds 1.0 ml/L 10% Yeast extract solution.
1 M Glycerin solution
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Glycerol | 1.0 MOLAR | JCM Medium 1125 lists 1.0 M Glycerol. |
JCM Medium 1125 adds 10.0 ml/L 1 M Glycerin solution.
5% Na2S x 9H2O solution
Dose: 5.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2S x 9H2O | 50.0 G_PER_L | JCM Medium 1125 lists 5% Na2S x 9H2O solution; this records the stock as 50.0 g/L Na2S x 9H2O. |
JCM Medium 1125 adds 5.0 ml/L 5% Na2S x 9H2O solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.121632Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1203 |
| 2026-01-28T19:48:15.426138Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:25.485512+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 12 solution(s) from ingredients |
| 2026-03-13T06:19:50.981512Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007730 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.390459+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Artficial Freshwater Medium II" to "artficial_freshwater_medium_ii" for consistent matching |
| 2026-05-16T09:03:08.393690+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:31.282863+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.200512+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m1200_m1204_score15.py | RESOLVED_TOGO_M1200_M1204_SCORE15 | TOGO M1203 records JCM Medium 1125 as Artficial Freshwater Medium II. JCM Medium 1125 / MediaDive J1125 supplies the complete base solution; JCM Media 187, 403, and 431 supply the FeCl2, Trace element, Selenite-tungstate, Vitamin, Thiamine, and Vitamin B12 stock compositions. |