Overview
- External ontology
- TOGO:M1220 — Natronospira Proteinivora Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1220 Original source: JCM - JCM_M1139 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1139
Composition
flowchart LR
CultureMech_007748["natronospira_proteinivora_medium"]
ing0_Basic_mineral_salt_medium_1__see_below_["Basic mineral salt medium 1 (see below)"]
CultureMech_007748 --> ing0_Basic_mineral_salt_medium_1__see_below_
ing1_Basal_medium_2__see_below_["Basal medium 2 (see below)"]
CultureMech_007748 --> ing1_Basal_medium_2__see_below_
ing2_Distilled_water["Distilled water"]
CultureMech_007748 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
ing3_NaCl["NaCl"]
CultureMech_007748 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_K2HPO4["K2HPO4"]
CultureMech_007748 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_007748 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_NaHCO3["NaHCO3"]
CultureMech_007748 --> ing6_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing6_NaHCO3 -.-> CHEBI_32139
ing7_Na2CO3["Na2CO3"]
CultureMech_007748 --> ing7_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing7_Na2CO3 -.-> CHEBI_29377
ing8_Casein["Casein"]
CultureMech_007748 --> ing8_Casein
ing9_NaOH["NaOH"]
CultureMech_007748 --> ing9_NaOH
CHEBI_32145["CHEBI:32145"]
ing9_NaOH -.-> CHEBI_32145
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Basic mineral salt medium 1 (see below) | 500 G_PER_L | ||
| 2 | Basal medium 2 (see below) | 500 G_PER_L | ||
| 3 |
Distilled water
H2O 18.015 g/mol
|
102.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Defined component, Inorganic compound [Merged 3 duplicates: 1.0, 1.0, 100.0] |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
256.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 240.0, 16.0] |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
4.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound [Merged 3 duplicates: 2.5, 2.0] |
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Simple component, Defined component, Inorganic compound |
| 7 |
NaHCO3
CHO3.Na 84.006 g/mol
|
30 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 8 |
Na2CO3
CO3.2Na 105.988 g/mol
|
190 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 9 | Casein | 5 G_PER_L | Role: Nitrogen source; Properties: Undefined component | |
| 10 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
Solutions
10% Tryptone solution (autoclaved)
Dose: 15 G_PER_L
Properties: Undefined component
1.0 Yeast extract solution (autoclaved)
Dose: 2 G_PER_L
Role: Nutrient source; Properties: Undefined component
Casein solution
Dose: 100 G_PER_L
1 M MgCl2 solution
Dose: 1 G_PER_L
Role: Mineral source
Trace element solution (see Medium [M1148])
Dose: 1 G_PER_L
Cross-reference to Medium M1148
2 M NH4Cl solution
Dose: 4 G_PER_L
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Solution
1 M MgCl2 solution
Dose: 1 G_PER_L
Role: Mineral source
Trace element solution (see Medium [M1148])
Dose: 2 G_PER_L
Cross-reference to Medium M1148
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1220_Natronospira_Proteinivora_Medium (CultureMech:007748)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:54.158628Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1220 |
| 2026-01-28T19:48:09.210533Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: K2HPO4; Added default concentration for ingredient: NaOH |
| 2026-03-09T08:04:14.865332+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 8 solution(s) from ingredients |
| 2026-03-13T03:08:42.021189Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 5 duplicate ingredient(s) |
| 2026-03-13T06:19:51.142615Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007748 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.557709+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Natronospira Proteinivora Medium" to "natronospira_proteinivora_medium" for consistent matching |
| 2026-05-16T09:03:08.572243+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:31.466990+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.272798+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:06.846666+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint f79f93dddea90e3dcb9374b1d87fd8a662a5846a3628eaee4ac934d0dad749d7 |