Overview
- External ontology
- TOGO:M1220 — Natronospira Proteinivora Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- JCM Medium 1139 defines Natronospira Proteinivora Medium as equal volumes of separately autoclaved Basic mineral salt medium 1 and Basal medium 2 plus autoclaved 10% tryptone and 1.0% yeast extract stocks. JCM Medium 1139 also prints a Casein solution subrecipe, but gives no Casein solution addition in the main recipe, so this record omits casein from the final medium.
Ingredients (0)
No ingredients recorded.
Preparation
- Prepare Basic mineral salt medium 1, bring it to 1.0 L, adjust pH to 6.8-6.9 with 1 M K2HPO4, autoclave, cool, then add autoclaved MgCl2 and JCM 1079 Trace element solutions.
- Prepare Basal medium 2, bring it to 1.0 L, confirm pH 9.9-10.0, autoclave, let stand for 3 days, decant the clear solution, then add autoclaved NH4Cl, MgCl2, and JCM 1079 Trace element solutions.
- Autoclave the 10% Tryptone and 1.0% Yeast extract solutions.
- Aseptically combine 500 ml Basic mineral salt medium 1, 500 ml Basal medium 2, 15 ml 10% Tryptone solution, and 2 ml 1.0% Yeast extract solution.
Solutions
Basic mineral salt medium 1
Dose: 500.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaCl | 240.0 G_PER_L | JCM Medium 1139 lists 240.0 g/L NaCl. |
| K2HPO4 | 2.5 G_PER_L | JCM Medium 1139 lists 2.5 g/L K2HPO4. |
| (NH4)2SO4 | 0.5 G_PER_L | JCM Medium 1139 lists 0.5 g/L (NH4)2SO4. |
| Distilled water | 1.0 L | JCM Medium 1139 lists 1.0 L Distilled water. |
| 1 M K2HPO4 solution | variable VARIABLE | JCM Medium 1139 adjusts Basic mineral salt medium 1 to pH 6.8-6.9 with 1 M K2HPO4; the volume is not specified. |
| 1 M MgCl2 solution | 1.0 ML_PER_L | JCM Medium 1139 adds 1.0 ml 1 M MgCl2 solution after autoclaving Basic mineral salt medium 1. |
| Trace element solution | 1.0 ML_PER_L | JCM Medium 1139 adds 1.0 ml JCM 1079 Trace element solution after autoclaving Basic mineral salt medium 1. |
JCM Medium 1139 adds 500.0 ml Basic mineral salt medium 1.
Basal medium 2
Dose: 500.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2CO3 | 190.0 G_PER_L | JCM Medium 1139 lists 190.0 g/L Na2CO3. |
| NaHCO3 | 30.0 G_PER_L | JCM Medium 1139 lists 30.0 g/L NaHCO3. |
| NaCl | 16.0 G_PER_L | JCM Medium 1139 lists 16.0 g/L NaCl. |
| K2HPO4 | 2.0 G_PER_L | JCM Medium 1139 lists 2.0 g/L K2HPO4. |
| Distilled water | 1.0 L | JCM Medium 1139 lists 1.0 L Distilled water. |
| 2 M NH4Cl solution | 4.0 ML_PER_L | JCM Medium 1139 adds 4.0 ml/L 2 M NH4Cl solution after Basal medium 2 is autoclaved and decanted. |
| 1 M MgCl2 solution | 1.0 ML_PER_L | JCM Medium 1139 adds 1.0 ml/L 1 M MgCl2 solution after Basal medium 2 is autoclaved and decanted. |
| Trace element solution | 2.0 ML_PER_L | JCM Medium 1139 adds 2.0 ml/L JCM 1079 Trace element solution after Basal medium 2 is autoclaved and decanted. |
JCM Medium 1139 adds 500.0 ml Basal medium 2.
10% Tryptone solution
Dose: 15.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Tryptone | 100.0 G_PER_L | JCM Medium 1139 lists a 10% Tryptone solution; this records the stock as 100.0 g/L. |
JCM Medium 1139 adds 15.0 ml 10% Tryptone solution.
1.0% Yeast extract solution
Dose: 2.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Yeast extract | 10.0 G_PER_L | JCM Medium 1139 lists a 1.0% Yeast extract solution; this records the stock as 10.0 g/L. |
JCM Medium 1139 adds 2.0 ml 1.0% Yeast extract solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:54.158628Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1220 |
| 2026-01-28T19:48:09.210533Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: K2HPO4; Added default concentration for ingredient: NaOH |
| 2026-03-09T08:04:14.865332+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 8 solution(s) from ingredients |
| 2026-03-13T03:08:42.021189Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 5 duplicate ingredient(s) |
| 2026-03-13T06:19:51.142615Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007748 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.557709+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Natronospira Proteinivora Medium" to "natronospira_proteinivora_medium" for consistent matching |
| 2026-05-16T09:03:08.572243+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:31.466990+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.272798+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m1220_score15.py | RESOLVED_TOGO_M1220_SCORE15 | JCM Medium 1139 defines Natronospira Proteinivora Medium as equal volumes of separately autoclaved Basic mineral salt medium 1 and Basal medium 2 plus autoclaved 10% tryptone and 1.0% yeast extract stocks. JCM Medium 1139 also prints a Casein solution subrecipe, but gives no Casein solution addition in the main recipe, so this record omits casein from the final medium. |