Overview
- External ontology
- TOGO:M1244 — NAS-02 Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1244 Original source: JCM - JCM_M1162 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1162
Composition
flowchart LR
CultureMech_007774["nas_02_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007774 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_KH2PO4["KH2PO4"]
CultureMech_007774 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_007774 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_Na2S2O3_5H2O["Na2S2O3・5H2O"]
CultureMech_007774 --> ing3_Na2S2O3_5H2O
CHEBI_32150["CHEBI:32150"]
ing3_Na2S2O3_5H2O -.-> CHEBI_32150
ing4_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007774 --> ing4_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing4_Carbon_dioxide_gas -.-> CHEBI_16526
ing5_Nitrogen_gas["Nitrogen gas"]
CultureMech_007774 --> ing5_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing5_Nitrogen_gas -.-> CHEBI_17997
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component |
| 4 |
Na2S2O3・5H2O
5H2O.2Na.O3S2 248.186 g/mol
|
2.5 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Defined component, Inorganic compound, Simple component |
| 5 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 6 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Solutions
Modified Allen's trace metal solution (see Medium [M1072])
Dose: 1 G_PER_L
Cross-reference to Medium M1072
5% (w/v) CaCl2・2H2O solution
Dose: 5 G_PER_L
Role: Mineral source; Properties: Defined component, Solution, Inorganic compound
10% (w/v) Yeast extract solution
Dose: 5 G_PER_L
Role: Nutrient source; Properties: Undefined component
5% (w/v) MgSO4・7H2O solution
Dose: 10 G_PER_L
Role: Mineral source; Properties: Defined component, Solution, Inorganic compound
130 mM FeCl2 solution
Dose: 10 G_PER_L
Role: Mineral source; Properties: Solution, Inorganic compound, Defined component
25 mM L-Cysteine・HCl solution
Dose: 10 G_PER_L
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1244_NAS-02_Medium (CultureMech:007774)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.208747Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1244 |
| 2026-01-28T19:48:12.601568Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:20.816370+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 7 solution(s) from ingredients |
| 2026-03-13T06:19:51.358799Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007774 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.782870+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NAS-02 Medium" to "nas_02_medium" for consistent matching |
| 2026-05-16T09:03:08.789373+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:31.694305+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.362769+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:07.068539+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint ca29d7a3d7e78fd4bb8adce937aa6908ad931c898a1bc18b5231649e61492f3d |