Overview
- External ontology
- TOGO:M1244 — NAS-02 Medium
- Applications
-
- Microbial cultivation
- pH
- 5.2
- Notes
- JCM Medium 1162 defines NAS-02 Medium with thiosulfate, Modified Allen's trace metal solution from JCM Medium 1014, N2-CO2 autoclaving at pH 5.2, and anaerobic post-autoclave additions of calcium, magnesium, trace vitamins, yeast extract, cysteine, and ferrous chloride stocks.
Composition
flowchart LR
CultureMech_007774["nas_02_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_007774 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_KH2PO4["KH2PO4"]
CultureMech_007774 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_Na2S2O3_x_5H2O["Na2S2O3 x 5H2O"]
CultureMech_007774 --> ing2_Na2S2O3_x_5H2O
CHEBI_32150["CHEBI:32150"]
ing2_Na2S2O3_x_5H2O -.-> CHEBI_32150
ing3_Distilled_water["Distilled water"]
CultureMech_007774 --> ing3_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing3_Distilled_water -.-> CHEBI_15377
ing4_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007774 --> ing4_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing4_Carbon_dioxide_gas -.-> CHEBI_16526
ing5_N2["N2"]
CultureMech_007774 --> ing5_N2
CHEBI_17997["CHEBI:17997"]
ing5_N2 -.-> CHEBI_17997
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.190295 G_PER_L |
CHEBI:62946
ammonium sulfate
|
JCM Medium 1162 lists 0.2 g (NH4)2SO4; MediaDive J1162 normalizes this to 0.190295 g/L. |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2.85442 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
JCM Medium 1162 lists 3.0 g KH2PO4; MediaDive J1162 normalizes this to 2.85442 g/L. |
| 3 |
Na2S2O3 x 5H2O
5H2O.2Na.O3S2 248.186 g/mol
|
2.37869 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
JCM Medium 1162 lists 2.5 g Na2S2O3 x 5H2O; MediaDive J1162 normalizes this to 2.37869 g/L. |
| 4 |
Distilled water
H2O 18.015 g/mol
|
1000.0 ML_PER_L |
CHEBI:15377
water
|
JCM Medium 1162 lists 1.0 L distilled water before stock additions. |
| 5 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
JCM Medium 1162 autoclaves NAS-02 medium under N2-CO2 (4:1, v/v). |
| 6 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
JCM Medium 1162 autoclaves NAS-02 medium under N2-CO2 (4:1, v/v). |
Preparation
- Mix the main salts, Modified Allen's trace metal solution, sodium thiosulfate, and distilled water thoroughly and adjust pH to 5.2.
- Autoclave the base medium under an N2-CO2 (4:1, v/v) gas mixture.
- After cooling, anaerobically and aseptically add the MgSO4, CaCl2, Trace vitamins, yeast extract, L-cysteine HCl, and FeCl2 solutions.
Solutions
Modified Allen's trace metal solution
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MnCl2 x 4H2O | 1.8 G_PER_L | JCM Medium 1014 lists 1.8 g/L MnCl2 x 4H2O. |
| Na2B4O7 x 10H2O | 4.5 G_PER_L | JCM Medium 1014 lists 4.5 g/L Na2B4O7 x 10H2O. |
| ZnSO4 x 7H2O | 0.22 G_PER_L | JCM Medium 1014 lists 0.22 g/L ZnSO4 x 7H2O. |
| CuCl2 x 2H2O | 0.05 G_PER_L | JCM Medium 1014 lists 0.05 g/L CuCl2 x 2H2O. |
| Na2MoO4 x 2H2O | 0.03 G_PER_L | JCM Medium 1014 lists 0.03 g/L Na2MoO4 x 2H2O. |
| VOSO4 x nH2O | 0.03 G_PER_L | JCM Medium 1014 lists 0.03 g/L VOSO4 x nH2O. |
| CoSO4 x 7H2O | 0.01 G_PER_L | JCM Medium 1014 lists 0.01 g/L CoSO4 x 7H2O. |
| Distilled water | 1.0 L | JCM Medium 1014 lists 1.0 L Distilled water. |
JCM Medium 1162 adds 1.0 ml/L Modified Allen's trace metal solution from JCM Medium 1014.
5% (w/v) MgSO4 x 7H2O solution
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MgSO4 x 7H2O | 50.0 G_PER_L | JCM Medium 1162 lists 5% (w/v) MgSO4 x 7H2O solution; this records the stock as 50.0 g/L MgSO4 x 7H2O. |
JCM Medium 1162 adds 10.0 ml/L 5% (w/v) MgSO4 x 7H2O solution.
5% (w/v) CaCl2 x 2H2O solution
Dose: 5.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| CaCl2 x 2H2O | 50.0 G_PER_L | JCM Medium 1162 lists 5% (w/v) CaCl2 x 2H2O solution; this records the stock as 50.0 g/L CaCl2 x 2H2O. |
JCM Medium 1162 adds 5.0 ml/L 5% (w/v) CaCl2 x 2H2O solution.
Trace vitamins
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 2.0 MG_PER_L | JCM Medium 197 lists 2.0 mg/L Biotin. |
| Folic acid | 2.0 MG_PER_L | JCM Medium 197 lists 2.0 mg/L Folic acid. |
| Pyridoxine HCl | 10.0 MG_PER_L | JCM Medium 197 lists 10.0 mg/L Pyridoxine HCl. |
| Thiamine HCl | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Thiamine HCl. |
| Riboflavin | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Riboflavin. |
| Nicotinic acid | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Nicotinic acid. |
| Calcium pantothenate | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Calcium pantothenate. |
| Vitamin B12 | 0.1 MG_PER_L | JCM Medium 197 lists 0.1 mg/L Vitamin B12. |
| p-Aminobenzoic acid | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L p-Aminobenzoic acid. |
| Lipoic acid | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Lipoic acid. |
| Distilled water | 1.0 L | JCM Medium 197 lists 1.0 L Distilled water. |
JCM Medium 1162 adds 10.0 ml/L filter-sterilized Trace vitamins from JCM Medium 197.
10% (w/v) Yeast extract solution
Dose: 5.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Yeast extract | 100.0 G_PER_L | JCM Medium 1162 lists 10% (w/v) Yeast extract solution; this records the stock as 100.0 g/L Yeast extract. |
JCM Medium 1162 adds 5.0 ml/L 10% (w/v) Yeast extract solution.
25 mM L-Cysteine HCl solution
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| L-Cysteine HCl | 25.0 MILLIMOLAR | JCM Medium 1162 lists 25 mM L-Cysteine HCl solution; this records the stock as 25.0 mM L-Cysteine HCl. |
JCM Medium 1162 adds 10.0 ml/L 25 mM L-Cysteine HCl solution.
130 mM FeCl2 solution
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| FeCl2 | 130.0 MILLIMOLAR | JCM Medium 1162 lists 130 mM FeCl2 solution; this records the stock as 130.0 mM FeCl2. |
JCM Medium 1162 adds 10.0 ml/L 130 mM FeCl2 solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.208747Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1244 |
| 2026-01-28T19:48:12.601568Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:20.816370+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 7 solution(s) from ingredients |
| 2026-03-13T06:19:51.358799Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007774 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.782870+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NAS-02 Medium" to "nas_02_medium" for consistent matching |
| 2026-05-16T09:03:08.789373+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:31.694305+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.362769+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m1244_score15.py | RESOLVED_TOGO_M1244_SCORE15 | JCM Medium 1162 defines NAS-02 Medium with thiosulfate, Modified Allen's trace metal solution from JCM Medium 1014, N2-CO2 autoclaving at pH 5.2, and anaerobic post-autoclave additions of calcium, magnesium, trace vitamins, yeast extract, cysteine, and ferrous chloride stocks. |