Overview
- External ontology
- TOGO:M1250 — LB (Luria-Bertani) Agar With Kanamycin And Rifampicin
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1250 Original source: JCM - JCM_M1168 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1168
Composition
flowchart LR
CultureMech_007781["lb_luria_bertani_agar_with_kanamycin_and_rifampicin"]
ing0_Distilled_water["Distilled water"]
CultureMech_007781 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_007781 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_Agar["Agar"]
CultureMech_007781 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
ing3_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_007781 --> ing3_Yeast_extract__BD_Difco_
ing4_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_007781 --> ing4_Tryptone__BD_Difco_
ing5_Kanamycin_sulfate__dissolved_in_distilled_water_["Kanamycin sulfate (dissolved in distilled water)"]
CultureMech_007781 --> ing5_Kanamycin_sulfate__dissolved_in_distilled_water_
ing6_Rifampicin__dissolved_in_methanol_["Rifampicin (dissolved in methanol)"]
CultureMech_007781 --> ing6_Rifampicin__dissolved_in_methanol_
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
10 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 4 | Yeast extract (BD-Difco) | 5 G_PER_L | Properties: Undefined component | |
| 5 | Tryptone (BD-Difco) | 10 G_PER_L | Properties: Undefined component | |
| 6 | Kanamycin sulfate (dissolved in distilled water) | 50 G_PER_L | Role: Antimicrobial agent; Properties: Solution, Organic compound, Defined component | |
| 7 | Rifampicin (dissolved in methanol) | 25 G_PER_L | Role: Antimicrobial agent; Properties: Defined component, Organic compound, Simple component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1250_LB_Luria-Bertani_Agar_With_Kanamycin_And_Rifampicin (CultureMech:007781)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.221040Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1250 |
| 2026-01-28T19:48:07.448497Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:51.415889Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007781 |
| 2026-03-17T06:29:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.838302+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LB (Luria-Bertani) Agar With Kanamycin And Rifampicin" to "lb_luria_bertani_agar_with_kanamycin_and_rifampicin" for consistent matching |
| 2026-06-05T07:40:31.751428+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:07.131777+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9161f654e8d9c8431d424c4ce35c76ee7e654b3bbbc27cc46450b9c250445e5f |