lb_luria_bertani_agar_with_kanamycin_and_rifampicin

CultureMech:007781 bacterial COMPLEX SOLID_AGAR
Original name: LB (Luria-Bertani) Agar With Kanamycin And Rifampicin

Overview

External ontology
TOGO:M1250 — LB (Luria-Bertani) Agar With Kanamycin And Rifampicin
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: https://togomedium.org/medium/M1250 Original source: JCM - JCM_M1168 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1168

Composition

flowchart LR
    CultureMech_007781["lb_luria_bertani_agar_with_kanamycin_and_rifampicin"]
    ing0_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
    CultureMech_007781 --> ing0_Tryptone__BD_Difco_
    ing1_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_007781 --> ing1_Yeast_extract__BD_Difco_
    ing2_NaCl["NaCl"]
    CultureMech_007781 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_Agar["Agar"]
    CultureMech_007781 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_Distilled_water["Distilled water"]
    CultureMech_007781 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377
    ing5_Kanamycin_sulfate["Kanamycin sulfate"]
    CultureMech_007781 --> ing5_Kanamycin_sulfate
    CHEBI_6109["CHEBI:6109"]
    ing5_Kanamycin_sulfate -.-> CHEBI_6109
    ing6_Rifampicin["Rifampicin"]
    CultureMech_007781 --> ing6_Rifampicin
    CHEBI_28077["CHEBI:28077"]
    ing6_Rifampicin -.-> CHEBI_28077

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Tryptone (BD-Difco) 10 G_PER_L MediaDive J1168 lists 10 g/L Tryptone with the BD-Difco attribute; the product is source-disclosed but not reducible to one ChEBI molecule.
2 Yeast extract (BD-Difco) 5 G_PER_L MediaDive J1168 lists 5 g/L Yeast extract with the BD-Difco attribute; the product is source-disclosed but not reducible to one ChEBI molecule.
3 NaCl
Cl.Na 58.443 g/mol
10 G_PER_L CHEBI:26710
sodium chloride
 MediaDive J1168 lists 10 g/L sodium chloride.
4 Agar 15 G_PER_L CHEBI:2509
agar
 MediaDive J1168 lists 15 g/L agar as the solidifying component.
5 Distilled water
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 MediaDive J1168 lists 1000 ml distilled water in the 1 L main solution.
6 Kanamycin sulfate
C18H36N4O11.H2O4S 582.582 g/mol
0.05 G_PER_L CHEBI:6109
kanamycin A sulfate
 MediaDive J1168 lists 50 mg Kanamycin sulfate, dissolved in distilled water and filter-sterilized before aseptic post-autoclave addition; 50 mg/L equals 0.05 g/L.
7 Rifampicin
C43H58N4O12 822.953 g/mol
0.025 G_PER_L CHEBI:28077
rifampicin
 MediaDive J1168 lists 25 mg Rifampicin, dissolved in methanol and filter-sterilized before aseptic post-autoclave addition; 25 mg/L equals 0.025 g/L.

Preparation

  1. Adjust the LB agar base to pH 7.0 before autoclaving.
  2. After autoclaving, aseptically add the kanamycin sulfate and rifampicin antibiotics as filter-sterilized stocks.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.221040Z togo-import Imported from TOGO Medium Source: JCM, ID: M1250
2026-01-28T19:48:07.448497Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:51.415889Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007781
2026-03-17T06:29:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:09.838302+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LB (Luria-Bertani) Agar With Kanamycin And Rifampicin" to "lb_luria_bertani_agar_with_kanamycin_and_rifampicin" for consistent matching
2026-06-05T07:40:31.751428+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m1250_score15.py RESOLVED_TOGO_M1250_SCORE15 MediaDive J1168 records pH 7.0 LB agar with 10 g/L Tryptone (BD-Difco), 5 g/L Yeast extract (BD-Difco), 10 g/L NaCl, 15 g/L agar, and filter-sterilized post-autoclave additions of 50 mg/L kanamycin sulfate and 25 mg/L rifampicin.