Overview
- External ontology
- TOGO:M125 — Rumen Fluid-Glucose-Cellobiose Agar
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M125 Original source: JCM - JCM_M134 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=134
Composition
flowchart LR
CultureMech_007791["rumen_fluid_glucose_cellobiose_agar"]
ing0_0_1__Vitamin_K1["0.1% Vitamin K1"]
CultureMech_007791 --> ing0_0_1__Vitamin_K1
ing1_Distilled_water["Distilled water"]
CultureMech_007791 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_007791 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_Cellobiose["Cellobiose"]
CultureMech_007791 --> ing3_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing3_Cellobiose -.-> CHEBI_17057
ing4_Rumen_fluid["Rumen fluid"]
CultureMech_007791 --> ing4_Rumen_fluid
ing5_Glucose["Glucose"]
CultureMech_007791 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Soluble_starch["Soluble starch"]
CultureMech_007791 --> ing6_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing6_Soluble_starch -.-> CHEBI_28017
ing7_Bacto_agar__BD_Difco_["Bacto agar (BD-Difco)"]
CultureMech_007791 --> ing7_Bacto_agar__BD_Difco_
ing8_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_007791 --> ing8_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L__Cysteine_HCl_H2O -.-> CHEBI_91248
ing9_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007791 --> ing9_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing9_MgSO4_7H2O -.-> CHEBI_31795
ing10_NaCl["NaCl"]
CultureMech_007791 --> ing10_NaCl
CHEBI_26710["CHEBI:26710"]
ing10_NaCl -.-> CHEBI_26710
ing11_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007791 --> ing11_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing11_CaCl2_2H2O -.-> CHEBI_86158
ing12_KH2PO4["KH2PO4"]
CultureMech_007791 --> ing12_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing12_KH2PO4 -.-> CHEBI_63036
ing13_K2HPO4["K2HPO4"]
CultureMech_007791 --> ing13_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing13_K2HPO4 -.-> CHEBI_131527
ing14_NaHCO3["NaHCO3"]
CultureMech_007791 --> ing14_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing14_NaHCO3 -.-> CHEBI_32139
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | 0.1% Vitamin K1 | 0.2 G_PER_L | Role: Vitamin; Properties: Organic compound, Solution, Defined component | |
| 2 |
Distilled water
H2O 18.015 g/mol
|
201.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 200.0, 1.0] |
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
Cellobiose
C12H22O11 342.297 g/mol
|
248 G_PER_L |
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 5 | Rumen fluid | 300 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
248 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 7 | Soluble starch | 0.5 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 8 | Bacto agar (BD-Difco) | 20 G_PER_L | Role: Solidifying component | |
| 9 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Organic compound, Simple component |
| 10 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 11 |
NaCl
Cl.Na 58.443 g/mol
|
2 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 12 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 13 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 14 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 15 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
Solutions
1% Resazurin solution
Dose: 4 G_PER_L
Role: pH dependent redox indicator; Properties: Defined component, Solution
1% Hemin solution
Dose: 10 G_PER_L
Role: Growth factor; Properties: Defined component, Solution, Organic compound
Salt solution (see below)
Dose: 500 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M125_Rumen_Fluid-Glucose-Cellobiose_Agar (CultureMech:007791)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.218681Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M125 |
| 2026-01-28T19:48:31.584685Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:52.626604+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:24.057501Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:51.499940Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007791 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:32.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.925810+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Rumen Fluid-Glucose-Cellobiose Agar" to "rumen_fluid_glucose_cellobiose_agar" for consistent matching |
| 2026-05-16T09:03:08.922452+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:31.837319+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:22.312306+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:47.421614+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:07.228428+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 804f6ce12d86913cdc97476c9e74976bc377806f9527b8f25d7ad1fe153c50a6 |