rumen_fluid_glucose_cellobiose_agar

CultureMech:007791 bacterial COMPLEX SOLID_AGAR
Original name: Rumen Fluid-Glucose-Cellobiose Agar

Overview

External ontology
TOGO:M125 — Rumen Fluid-Glucose-Cellobiose Agar
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M125 Original source: JCM - JCM_M134 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=134

Composition

flowchart LR
    CultureMech_007791["rumen_fluid_glucose_cellobiose_agar"]
    ing0_0_1__Vitamin_K1["0.1% Vitamin K1"]
    CultureMech_007791 --> ing0_0_1__Vitamin_K1
    ing1_Distilled_water["Distilled water"]
    CultureMech_007791 --> ing1_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Distilled_water -.-> CHEBI_15377
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_007791 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_Cellobiose["Cellobiose"]
    CultureMech_007791 --> ing3_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing3_Cellobiose -.-> CHEBI_17057
    ing4_Rumen_fluid["Rumen fluid"]
    CultureMech_007791 --> ing4_Rumen_fluid
    ing5_Glucose["Glucose"]
    CultureMech_007791 --> ing5_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing5_Glucose -.-> CHEBI_17234
    ing6_Soluble_starch["Soluble starch"]
    CultureMech_007791 --> ing6_Soluble_starch
    CHEBI_28017["CHEBI:28017"]
    ing6_Soluble_starch -.-> CHEBI_28017
    ing7_Bacto_agar__BD_Difco_["Bacto agar (BD-Difco)"]
    CultureMech_007791 --> ing7_Bacto_agar__BD_Difco_
    ing8_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
    CultureMech_007791 --> ing8_L__Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing8_L__Cysteine_HCl_H2O -.-> CHEBI_91248
    ing9_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_007791 --> ing9_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing9_MgSO4_7H2O -.-> CHEBI_31795
    ing10_NaCl["NaCl"]
    CultureMech_007791 --> ing10_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing10_NaCl -.-> CHEBI_26710
    ing11_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_007791 --> ing11_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing11_CaCl2_2H2O -.-> CHEBI_86158
    ing12_KH2PO4["KH2PO4"]
    CultureMech_007791 --> ing12_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing12_KH2PO4 -.-> CHEBI_63036
    ing13_K2HPO4["K2HPO4"]
    CultureMech_007791 --> ing13_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing13_K2HPO4 -.-> CHEBI_131527
    ing14_NaHCO3["NaHCO3"]
    CultureMech_007791 --> ing14_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing14_NaHCO3 -.-> CHEBI_32139

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 0.1% Vitamin K1 0.2 G_PER_L Role: Vitamin; Properties: Organic compound, Solution, Defined component
2 Distilled water
H2O 18.015 g/mol
201.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 200.0, 1.0]
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1 G_PER_L CHEBI:62946
ammonium sulfate
 Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound
4 Cellobiose
C12H22O11 342.297 g/mol
248 G_PER_L CHEBI:17057
cellobiose
 Role: Carbon source; Properties: Organic compound, Defined component, Simple component
5 Rumen fluid 300 G_PER_L Role: Nutrient source; Properties: Undefined component
6 Glucose
C6H12O6 180.156 g/mol
248 G_PER_L CHEBI:17234
glucose
 Role: Carbon source; Properties: Defined component, Simple component, Organic compound
7 Soluble starch 0.5 G_PER_L CHEBI:28017
starch
 Role: Carbon source; Properties: Undefined component
8 Bacto agar (BD-Difco) 20 G_PER_L Role: Solidifying component
9 L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
 Properties: Defined component, Organic compound, Simple component
10 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
11 NaCl
Cl.Na 58.443 g/mol
2 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
12 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.2 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
13 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
14 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
15 NaHCO3
CHO3.Na 84.006 g/mol
10 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component

Solutions

1% Resazurin solution

Dose: 4 G_PER_L

Role: pH dependent redox indicator; Properties: Defined component, Solution

1% Hemin solution

Dose: 10 G_PER_L

Role: Growth factor; Properties: Defined component, Solution, Organic compound

Salt solution (see below)

Dose: 500 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.218681Z togo-import Imported from TOGO Medium Source: JCM, ID: M125
2026-01-28T19:48:31.584685Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:52.626604+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T03:09:24.057501Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:51.499940Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007791
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:32.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:09.925810+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Rumen Fluid-Glucose-Cellobiose Agar" to "rumen_fluid_glucose_cellobiose_agar" for consistent matching
2026-05-16T09:03:08.922452+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:31.837319+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:22.312306+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:47.421614+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.