rumen_fluid_xylan_agar

CultureMech:007802 bacterial COMPLEX SOLID_AGAR
Original name: Rumen Fluid-Xylan-Agar

Overview

External ontology
TOGO:M126 — Rumen Fluid-Xylan-Agar
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M126 Original source: JCM - JCM_M135 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=135

Composition

flowchart LR
    CultureMech_007802["rumen_fluid_xylan_agar"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007802 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_007802 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_007802 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_KH2PO4["KH2PO4"]
    CultureMech_007802 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_K2HPO4["K2HPO4"]
    CultureMech_007802 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_Na2S_9H2O["Na2S・9H2O"]
    CultureMech_007802 --> ing5_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing5_Na2S_9H2O -.-> CHEBI_76209
    ing6_NaHCO3["NaHCO3"]
    CultureMech_007802 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7__NH4_2SO4["(NH4)2SO4"]
    CultureMech_007802 --> ing7__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing7__NH4_2SO4 -.-> CHEBI_62946
    ing8_CaCl2["CaCl2"]
    CultureMech_007802 --> ing8_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing8_CaCl2 -.-> CHEBI_3312
    ing9_Xylan["Xylan"]
    CultureMech_007802 --> ing9_Xylan
    CHEBI_37166["CHEBI:37166"]
    ing9_Xylan -.-> CHEBI_37166
    ing10_Indigo_carmine["Indigo carmine"]
    CultureMech_007802 --> ing10_Indigo_carmine
    CHEBI_31695["CHEBI:31695"]
    ing10_Indigo_carmine -.-> CHEBI_31695
    ing11_Rumen_fluid["Rumen fluid"]
    CultureMech_007802 --> ing11_Rumen_fluid
    ing12_Agar["Agar"]
    CultureMech_007802 --> ing12_Agar
    CHEBI_2509["CHEBI:2509"]
    ing12_Agar -.-> CHEBI_2509
    ing13_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
    CultureMech_007802 --> ing13_L__Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing13_L__Cysteine_HCl_H2O -.-> CHEBI_91248

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
600 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
90 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
3 NaCl
Cl.Na 58.443 g/mol
0.45 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
4 KH2PO4
H2O4P.K 136.084 g/mol
225 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
5 K2HPO4
HO4P.2K 174.174 g/mol
225 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
Role: Buffer; Properties: Simple component, Defined component, Inorganic compound
6 Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
0.25 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
Role: Reducing agent; Properties: Simple component, Inorganic compound, Defined component
7 NaHCO3
CHO3.Na 84.006 g/mol
6.37 G_PER_L CHEBI:32139
sodium hydrogencarbonate
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
8 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.45 G_PER_L CHEBI:62946
ammonium sulfate
Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component
9 CaCl2
Ca.2Cl 110.984 g/mol
0.45 G_PER_L CHEBI:3312
calcium dichloride
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
10 Xylan 15 G_PER_L CHEBI:37166
xylan
Role: Carbon source; Properties: Undefined component
11 Indigo carmine
C16H8N2O8S2.2Na 466.36 g/mol
5 G_PER_L CHEBI:31695
indigo carmine
Role: pH indicator; Properties: Organic compound, Simple component, Defined component
12 Rumen fluid 400 G_PER_L Role: Nutrient source; Properties: Undefined component
13 Agar 15 G_PER_L CHEBI:2509
agar
Role: Solidifying component
14 L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.25 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
Properties: Defined component, Organic compound, Simple component

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:54.239600Z togo-import Imported from TOGO Medium Source: JCM, ID: M126
2026-01-28T19:48:18.944446Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:51.590690Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007802
2026-03-17T06:30:05.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:10.020010+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Rumen Fluid-Xylan-Agar" to "rumen_fluid_xylan_agar" for consistent matching
2026-06-05T07:40:31.930189+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:22.380652+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:47.453480+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:07.305895+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 9a40d0785a64b3842d4e32b20227e5aafad0202277d5cfa4184495a7fbba2ac0