Overview
- External ontology
- TOGO:M126 — Rumen Fluid-Xylan-Agar
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M126 Original source: JCM - JCM_M135 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=135
Composition
flowchart LR
CultureMech_007802["rumen_fluid_xylan_agar"]
ing0_Distilled_water["Distilled water"]
CultureMech_007802 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007802 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_007802 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_KH2PO4["KH2PO4"]
CultureMech_007802 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_K2HPO4["K2HPO4"]
CultureMech_007802 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_Na2S_9H2O["Na2S・9H2O"]
CultureMech_007802 --> ing5_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing5_Na2S_9H2O -.-> CHEBI_76209
ing6_NaHCO3["NaHCO3"]
CultureMech_007802 --> ing6_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing6_NaHCO3 -.-> CHEBI_32139
ing7__NH4_2SO4["(NH4)2SO4"]
CultureMech_007802 --> ing7__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing7__NH4_2SO4 -.-> CHEBI_62946
ing8_CaCl2["CaCl2"]
CultureMech_007802 --> ing8_CaCl2
CHEBI_3312["CHEBI:3312"]
ing8_CaCl2 -.-> CHEBI_3312
ing9_Xylan["Xylan"]
CultureMech_007802 --> ing9_Xylan
CHEBI_37166["CHEBI:37166"]
ing9_Xylan -.-> CHEBI_37166
ing10_Indigo_carmine["Indigo carmine"]
CultureMech_007802 --> ing10_Indigo_carmine
CHEBI_31695["CHEBI:31695"]
ing10_Indigo_carmine -.-> CHEBI_31695
ing11_Rumen_fluid["Rumen fluid"]
CultureMech_007802 --> ing11_Rumen_fluid
ing12_Agar["Agar"]
CultureMech_007802 --> ing12_Agar
CHEBI_2509["CHEBI:2509"]
ing12_Agar -.-> CHEBI_2509
ing13_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_007802 --> ing13_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing13_L__Cysteine_HCl_H2O -.-> CHEBI_91248
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
600 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
90 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
0.45 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
225 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
225 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Simple component, Defined component, Inorganic compound |
| 6 |
Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
|
0.25 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Simple component, Inorganic compound, Defined component |
| 7 |
NaHCO3
CHO3.Na 84.006 g/mol
|
6.37 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 8 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.45 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component |
| 9 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.45 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 10 | Xylan | 15 G_PER_L |
CHEBI:37166
xylan
|
Role: Carbon source; Properties: Undefined component |
| 11 |
Indigo carmine
C16H8N2O8S2.2Na 466.36 g/mol
|
5 G_PER_L |
CHEBI:31695
indigo carmine
|
Role: pH indicator; Properties: Organic compound, Simple component, Defined component |
| 12 | Rumen fluid | 400 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 13 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 14 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.25 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Organic compound, Simple component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.239600Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M126 |
| 2026-01-28T19:48:18.944446Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:51.590690Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007802 |
| 2026-03-17T06:30:05.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.020010+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Rumen Fluid-Xylan-Agar" to "rumen_fluid_xylan_agar" for consistent matching |
| 2026-06-05T07:40:31.930189+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:22.380652+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:47.453480+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |