Overview
- External ontology
- TOGO:M1274 — Modified Marine Agar 2216 With 5% NaCl (pH 9)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1274 Original source: JCM - JCM_M1189 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1189
Composition
flowchart LR
CultureMech_007807["modified_marine_agar_2216_with_5_nacl_ph_9"]
ing0_Distilled_water["Distilled water"]
CultureMech_007807 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_007807 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_Malt_extract__BD_Difco_["Malt extract (BD-Difco)"]
CultureMech_007807 --> ing2_Malt_extract__BD_Difco_
ing3_Casitone__BD_Difco_["Casitone (BD-Difco)"]
CultureMech_007807 --> ing3_Casitone__BD_Difco_
ing4_Soytone__BD_Difco__or_Phytone_peptone__BD_BBL_["Soytone (BD-Difco) or Phytone peptone (BD-BBL)"]
CultureMech_007807 --> ing4_Soytone__BD_Difco__or_Phytone_peptone__BD_BBL_
ing5_Marine_agar_2216__BD_Difco_["Marine agar 2216 (BD-Difco)"]
CultureMech_007807 --> ing5_Marine_agar_2216__BD_Difco_
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
990 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
50 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 | Malt extract (BD-Difco) | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 | Casitone (BD-Difco) | 1 G_PER_L | Properties: Undefined component | |
| 5 | Soytone (BD-Difco) or Phytone peptone (BD-BBL) | 1 G_PER_L | Properties: Undefined component | |
| 6 | Marine agar 2216 (BD-Difco) | 55.1 G_PER_L | Properties: Undefined component |
Solutions
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Na2CO3 solution
Dose: variable VARIABLE
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_marine_agar_2216_with_5_nacl_ph_9 (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.269694Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1274 |
| 2026-01-28T19:48:11.534730Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Na2CO3 solution |
| 2026-03-09T08:04:18.900862+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:19:51.623589Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007807 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.055360+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified Marine Agar 2216 With 5% NaCl (pH 9)" to "modified_marine_agar_2216_with_5_nacl_ph_9" for consistent matching |
| 2026-05-16T09:04:25.646089+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:37.029618+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:48.742485+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint fc07982eab48566c783fc24575f9d364b974013a0f44451a0910cb0ba3ac67e6 |