modified_marine_agar_2216_with_5_nacl_ph_9

CultureMech:007807 bacterial COMPLEX SOLID_AGAR
Original name: Modified Marine Agar 2216 With 5% NaCl (pH 9)

Overview

External ontology
TOGO:M1274 — Modified Marine Agar 2216 With 5% NaCl (pH 9)
Applications
  • Microbial cultivation
pH
9.0
Notes
JCM Medium 1189 Modified Marine Agar 2216 with 5% NaCl (pH 9) lists the JCM 913 marine-agar base plus 50.0 g/L NaCl, 10.0 ml/L Trace vitamins, 990.0 ml/L Distilled water, and pH adjustment to 9.0 with sterilized 10% Na2CO3 solution.

Composition

flowchart LR
    CultureMech_007807["modified_marine_agar_2216_with_5_nacl_ph_9"]
    ing0_Marine_agar_2216__BD_Difco_["Marine agar 2216 (BD-Difco)"]
    CultureMech_007807 --> ing0_Marine_agar_2216__BD_Difco_
    ing1_Casitone__BD_Difco_["Casitone (BD-Difco)"]
    CultureMech_007807 --> ing1_Casitone__BD_Difco_
    ing2_Soytone__BD_Difco__or_Phytone_peptone__BD_BBL_["Soytone (BD-Difco) or Phytone peptone (BD-BBL)"]
    CultureMech_007807 --> ing2_Soytone__BD_Difco__or_Phytone_peptone__BD_BBL_
    ing3_Malt_extract__BD_Difco_["Malt extract (BD-Difco)"]
    CultureMech_007807 --> ing3_Malt_extract__BD_Difco_
    ing4_NaCl["NaCl"]
    CultureMech_007807 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_Distilled_water["Distilled water"]
    CultureMech_007807 --> ing5_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing5_Distilled_water -.-> CHEBI_15377

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Marine agar 2216 (BD-Difco) 55.1 G_PER_L TOGO M1274 / JCM Medium 1189 lists 55.1 g/L Marine agar 2216 (BD-Difco).
2 Casitone (BD-Difco) 1.0 G_PER_L TOGO M1274 / JCM Medium 1189 lists 1.0 g/L Casitone (BD-Difco).
3 Soytone (BD-Difco) or Phytone peptone (BD-BBL) 1.0 G_PER_L TOGO M1274 / JCM Medium 1189 lists 1.0 g/L Soytone (BD-Difco) or Phytone peptone (BD-BBL).
4 Malt extract (BD-Difco) 1.0 G_PER_L FOODON:03301056
malt extract
 TOGO M1274 / JCM Medium 1189 lists 1.0 g/L Malt extract (BD-Difco).
5 NaCl
Cl.Na 58.443 g/mol
50.0 G_PER_L CHEBI:26710
sodium chloride
 TOGO M1274 / JCM Medium 1189 lists 50.0 g/L NaCl.
6 Distilled water
H2O 18.015 g/mol
990.0 ML_PER_L CHEBI:15377
water
 TOGO M1274 / JCM Medium 1189 lists 990.0 ml/L Distilled water.

Preparation

  1. Combine the Modified Marine Agar 2216 components listed by TOGO M1274 / JCM Medium 1189.
  2. Adjust pH to 9.0.

Solutions

Trace vitamins

Dose: 10.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Biotin 2.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 2.0 mg/L Biotin.
p-Aminobenzoic acid 5.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 5.0 mg/L p-Aminobenzoic acid.
Thiamine HCl 5.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 5.0 mg/L Thiamine HCl.
Calcium pantothenate 5.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 5.0 mg/L Calcium pantothenate.
Pyridoxine HCl 10.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 10.0 mg/L Pyridoxine HCl.
Folic acid 2.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 2.0 mg/L Folic acid.
Vitamin B12 0.1 MG_PER_L TOGO M190 / JCM Medium 197 lists 0.1 mg/L Vitamin B12.
Riboflavin 5.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 5.0 mg/L Riboflavin.
Nicotinic acid 5.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 5.0 mg/L Nicotinic acid.
Lipoic acid 5.0 MG_PER_L TOGO M190 / JCM Medium 197 lists 5.0 mg/L Lipoic acid.
Distilled water 1000.0 ML_PER_L TOGO M190 / JCM Medium 197 lists 1000.0 ml/L Distilled water.
TOGO M1274 / JCM Medium 1189 adds 10 ml/L Trace vitamins from TOGO M190/JCM Medium 197.

10% Na2CO3 solution

Dose: variable VARIABLE

Stock composition
IngredientConcentrationNotes
Na2CO3 10.0 PERCENT_W_V TOGO M1274 / JCM Medium 1189 specifies the pH-adjustment Na2CO3 solution as 10% w/v.
TOGO M1274 / JCM Medium 1189 adjusts the medium to pH 9.0 with sterilized 10% Na2CO3 solution.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.269694Z togo-import Imported from TOGO Medium Source: JCM, ID: M1274
2026-01-28T19:48:11.534730Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Na2CO3 solution
2026-03-09T08:04:18.900862+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T06:19:51.623589Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007807
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:29:49.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:10.055360+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified Marine Agar 2216 With 5% NaCl (pH 9)" to "modified_marine_agar_2216_with_5_nacl_ph_9" for consistent matching
2026-05-16T09:04:25.646089+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:41:37.029618+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m958_modified_marine_score15.py RESOLVED_TOGO_M1274_MODIFIED_MARINE_NACL_PH9 Corrected JCM Modified Marine Agar 2216 water units, added the source pH, grounded the disclosed complex nutrient products where possible, and marked remaining BD products as intentionally unmapped source components.