Overview
- External ontology
- TOGO:M1296 — Halanaerobium Hydrogeniformans Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1296 Original source: JCM - JCM_M1209 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1209
Composition
flowchart LR
CultureMech_007829["halanaerobium_hydrogeniformans_medium"]
ing0_N2["N2"]
CultureMech_007829 --> ing0_N2
CHEBI_17997["CHEBI:17997"]
ing0_N2 -.-> CHEBI_17997
ing1_Distilled_water["Distilled water"]
CultureMech_007829 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_NaCl["NaCl"]
CultureMech_007829 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_Na2CO3["Na2CO3"]
CultureMech_007829 --> ing3_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing3_Na2CO3 -.-> CHEBI_29377
ing4_K2HPO4["K2HPO4"]
CultureMech_007829 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_007829 --> ing5_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_2H2O -.-> CHEBI_86158
ing6_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_007829 --> ing6_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_6H2O -.-> CHEBI_86345
ing7_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_007829 --> ing7_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing7_FeSO4_7H2O -.-> CHEBI_75836
ing8_MnSO4_xH2O["MnSO4·xH2O"]
CultureMech_007829 --> ing8_MnSO4_xH2O
ing9_NH4NO3["NH4NO3"]
CultureMech_007829 --> ing9_NH4NO3
CHEBI_63038["CHEBI:63038"]
ing9_NH4NO3 -.-> CHEBI_63038
ing10_SiO2["SiO2"]
CultureMech_007829 --> ing10_SiO2
CHEBI_30563["CHEBI:30563"]
ing10_SiO2 -.-> CHEBI_30563
ing11_Methylene_blue["Methylene blue"]
CultureMech_007829 --> ing11_Methylene_blue
CHEBI_6872["CHEBI:6872"]
ing11_Methylene_blue -.-> CHEBI_6872
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 2 |
Distilled water
H2O 18.015 g/mol
|
881.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 3 duplicates: 500.0, 380.0, 1.0] |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
70 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
Na2CO3
CO3.2Na 105.988 g/mol
|
40 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
6.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 6 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
4.2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 7 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
8.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 8 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
1.8 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 9 | MnSO4·xH2O | 4.5 G_PER_L | Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component | |
| 10 |
NH4NO3
H4N.NO3 80.043 g/mol
|
50 G_PER_L |
CHEBI:63038
ammonium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
SiO2
O2Si 60.084 g/mol
|
7.5 G_PER_L |
CHEBI:30563
silicon dioxide
|
Role: Mineral source |
| 12 |
Methylene blue
C16H18N3S.Cl 319.861 g/mol
|
4 G_PER_L |
CHEBI:6872
methylene blue
|
Properties: Defined component, Simple component |
Solutions
Solution A
Dose: 500 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 400 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
10% Yeast extract solution
Dose: 10 G_PER_L
Role: Nutrient source; Properties: Undefined component
25% (w/v) Glycerin solution
Dose: 100 G_PER_L
Role: Carbon source; Properties: Solution, Defined component, Organic compound
5% Na2S·9H2O solution
Dose: 15 G_PER_L
Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound
5% L-Cysteine·HCl·H2O solution
Dose: 12 G_PER_L
Properties: Defined component, Organic compound, Solution
Basal salts solution (see below)
Dose: 10 G_PER_L
Trace minerals (see Medium [M142])
Dose: 10 G_PER_L
Cross-reference to Medium M142
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1296_Halanaerobium_Hydrogeniformans_Medium (CultureMech:007829)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.304726Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1296 |
| 2026-01-28T19:48:14.857622Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2; Added default concentration for ingredient: N2; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:24.721555+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 8 solution(s) from ingredients |
| 2026-03-13T03:08:53.156012Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:51.793367Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007829 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.229328+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Halanaerobium Hydrogeniformans Medium" to "halanaerobium_hydrogeniformans_medium" for consistent matching |
| 2026-05-16T09:03:09.195474+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:32.128605+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.513540+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:07.650505+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint f05814d96890e28d597519fc04598b26ed7e19e34a295b14efca987aaa6c93c6 |