halanaerobium_hydrogeniformans_medium

CultureMech:007829 bacterial COMPLEX LIQUID
Original name: Halanaerobium Hydrogeniformans Medium

Overview

External ontology
TOGO:M1296 — Halanaerobium Hydrogeniformans Medium
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M1296 Original source: JCM - JCM_M1209 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1209

Composition

flowchart LR
    CultureMech_007829["halanaerobium_hydrogeniformans_medium"]
    ing0_N2["N2"]
    CultureMech_007829 --> ing0_N2
    CHEBI_17997["CHEBI:17997"]
    ing0_N2 -.-> CHEBI_17997
    ing1_Distilled_water["Distilled water"]
    CultureMech_007829 --> ing1_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Distilled_water -.-> CHEBI_15377
    ing2_NaCl["NaCl"]
    CultureMech_007829 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_Na2CO3["Na2CO3"]
    CultureMech_007829 --> ing3_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing3_Na2CO3 -.-> CHEBI_29377
    ing4_K2HPO4["K2HPO4"]
    CultureMech_007829 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_007829 --> ing5_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_2H2O -.-> CHEBI_86158
    ing6_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_007829 --> ing6_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_6H2O -.-> CHEBI_86345
    ing7_FeSO4_7H2O["FeSO4·7H2O"]
    CultureMech_007829 --> ing7_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_7H2O -.-> CHEBI_75836
    ing8_MnSO4_xH2O["MnSO4·xH2O"]
    CultureMech_007829 --> ing8_MnSO4_xH2O
    ing9_NH4NO3["NH4NO3"]
    CultureMech_007829 --> ing9_NH4NO3
    CHEBI_63038["CHEBI:63038"]
    ing9_NH4NO3 -.-> CHEBI_63038
    ing10_SiO2["SiO2"]
    CultureMech_007829 --> ing10_SiO2
    CHEBI_30563["CHEBI:30563"]
    ing10_SiO2 -.-> CHEBI_30563
    ing11_Methylene_blue["Methylene blue"]
    CultureMech_007829 --> ing11_Methylene_blue
    CHEBI_6872["CHEBI:6872"]
    ing11_Methylene_blue -.-> CHEBI_6872

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
2 Distilled water
H2O 18.015 g/mol
881.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 3 duplicates: 500.0, 380.0, 1.0]
3 NaCl
Cl.Na 58.443 g/mol
70 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
4 Na2CO3
CO3.2Na 105.988 g/mol
40 G_PER_L CHEBI:29377
sodium carbonate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
5 K2HPO4
HO4P.2K 174.174 g/mol
6.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
6 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
4.2 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
7 MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
8.5 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
8 FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
1.8 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
9 MnSO4·xH2O 4.5 G_PER_L Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
10 NH4NO3
H4N.NO3 80.043 g/mol
50 G_PER_L CHEBI:63038
ammonium nitrate
 Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
11 SiO2
O2Si 60.084 g/mol
7.5 G_PER_L CHEBI:30563
silicon dioxide
 Role: Mineral source
12 Methylene blue
C16H18N3S.Cl 319.861 g/mol
4 G_PER_L CHEBI:6872
methylene blue
 Properties: Defined component, Simple component

Solutions

Solution A

Dose: 500 G_PER_L

See mediadive_5342_Solution_A.yaml for composition

Solution B

Dose: 400 G_PER_L

See mediadive_5343_Solution_B.yaml for composition

10% Yeast extract solution

Dose: 10 G_PER_L

Role: Nutrient source; Properties: Undefined component

25% (w/v) Glycerin solution

Dose: 100 G_PER_L

Role: Carbon source; Properties: Solution, Defined component, Organic compound

5% Na2S·9H2O solution

Dose: 15 G_PER_L

Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound

5% L-Cysteine·HCl·H2O solution

Dose: 12 G_PER_L

Properties: Defined component, Organic compound, Solution

Basal salts solution (see below)

Dose: 10 G_PER_L

Trace minerals (see Medium [M142])

Dose: 10 G_PER_L

Cross-reference to Medium M142

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.304726Z togo-import Imported from TOGO Medium Source: JCM, ID: M1296
2026-01-28T19:48:14.857622Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: N2; Added default concentration for ingredient: N2; Added default concentration for ingredient: N2
2026-03-09T08:04:24.721555+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 8 solution(s) from ingredients
2026-03-13T03:08:53.156012Z data-quality-cleanup-v1.0 Data quality fixes Merged 4 duplicate ingredient(s)
2026-03-13T06:19:51.793367Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007829
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:57.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:10.229328+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Halanaerobium Hydrogeniformans Medium" to "halanaerobium_hydrogeniformans_medium" for consistent matching
2026-05-16T09:03:09.195474+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:32.128605+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:47.513540+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.