Overview
- External ontology
- TOGO:M1312 — Modified Nitrate Mineral Salts Medium-2
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1312 Original source: JCM - JCM_M1221 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1221
Composition
flowchart LR
CultureMech_007848["modified_nitrate_mineral_salts_medium_2"]
ing0_Distilled_water["Distilled water"]
CultureMech_007848 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007848 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007848 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KNO3["KNO3"]
CultureMech_007848 --> ing3_KNO3
CHEBI_63043["CHEBI:63043"]
ing3_KNO3 -.-> CHEBI_63043
ing4_Ammonium_ferric_citrate["Ammonium ferric citrate"]
CultureMech_007848 --> ing4_Ammonium_ferric_citrate
CHEBI_31604["CHEBI:31604"]
ing4_Ammonium_ferric_citrate -.-> CHEBI_31604
ing5_Methane_gas["Methane gas"]
CultureMech_007848 --> ing5_Methane_gas
ing6_Air["Air"]
CultureMech_007848 --> ing6_Air
ing7_KH2PO4["KH2PO4"]
CultureMech_007848 --> ing7_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing7_KH2PO4 -.-> CHEBI_63036
ing8_Na2HPO4_2H2O["Na2HPO4・2H2O"]
CultureMech_007848 --> ing8_Na2HPO4_2H2O
CHEBI_34683["CHEBI:34683"]
ing8_Na2HPO4_2H2O -.-> CHEBI_34683
ing9_Vitamin_B12["Vitamin B12"]
CultureMech_007848 --> ing9_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing9_Vitamin_B12 -.-> CHEBI_176843
ing10_Nicotinamide["Nicotinamide"]
CultureMech_007848 --> ing10_Nicotinamide
CHEBI_17154["CHEBI:17154"]
ing10_Nicotinamide -.-> CHEBI_17154
ing11_L_Ascorbic_acid["L-Ascorbic acid"]
CultureMech_007848 --> ing11_L_Ascorbic_acid
CHEBI_29073["CHEBI:29073"]
ing11_L_Ascorbic_acid -.-> CHEBI_29073
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
101.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 2 duplicates: 1.0, 100.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
KNO3
K.NO3 101.102 g/mol
|
0.25 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 5 | Ammonium ferric citrate | 4 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
Role: Nitrogen source; Properties: Organic compound, Simple component, Defined component |
| 6 | Methane gas | variable VARIABLE | Properties: ガス | |
| 7 | Air | variable VARIABLE | Properties: ガス | |
| 8 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.4 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 9 |
Na2HPO4・2H2O
HO4P.2Na 141.958 g/mol
|
3.6 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 10 | Vitamin B12 | 20 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Simple component, Organic compound, Defined component |
| 11 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
5 G_PER_L |
CHEBI:17154
nicotinamide
|
Role: Growth factor; Properties: Organic compound, Simple component, Defined component |
| 12 |
L-Ascorbic acid
C6H8O6 176.124 g/mol
|
5 G_PER_L |
CHEBI:29073
L-ascorbic acid
|
Role: Vitamin; Properties: Defined component, Simple component, Organic compound |
Solutions
1 M HEPES solution (pH 7.0)
Dose: 2 G_PER_L
Role: Buffer; Properties: Defined component, Solution
FeCl2 solution (see Medium [M180])
Dose: 1 G_PER_L
Cross-reference to Medium M180
Trace element solution (see Medium [M180])
Dose: 1 G_PER_L
Cross-reference to Medium M180
Phosphate buffer solution (see below)
Dose: 2 G_PER_L
Vitamin solution (see below)
Dose: 10 G_PER_L
Trace vitamins (see Medium [M190])
Dose: 1 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1312_Modified_Nitrate_Mineral_Salts_Medium-2 (CultureMech:007848)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.338840Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1312 |
| 2026-01-28T19:48:34.460663Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Methane gas; Added default concentration for ingredient: Air |
| 2026-03-09T08:04:57.233408+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T03:09:28.812249Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:51.938296Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007848 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:38.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.379715+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified Nitrate Mineral Salts Medium-2" to "modified_nitrate_mineral_salts_medium_2" for consistent matching |
| 2026-05-16T09:03:09.326790+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:32.282029+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:22.654083+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:47.564971+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:07.919623+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint e610b303ea7f5b9c20d31584a6660aef36aed9dc9df2adae304dca0066b4f01c |