modified_nitrate_mineral_salts_medium_2 (CultureMech:007848)

Overview

External ontology

TOGO:M1312 — Modified Nitrate Mineral Salts Medium-2

Applications

Microbial cultivation

Notes

Source: https://togomedium.org/medium/M1312 Original source: JCM - JCM_M1221 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1221

Composition

flowchart LR
    CultureMech_007848["modified_nitrate_mineral_salts_medium_2"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007848 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_007848 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_007848 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_KNO3["KNO3"]
    CultureMech_007848 --> ing3_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing3_KNO3 -.-> CHEBI_63043
    ing4_Ammonium_ferric_citrate["Ammonium ferric citrate"]
    CultureMech_007848 --> ing4_Ammonium_ferric_citrate
    CHEBI_31604["CHEBI:31604"]
    ing4_Ammonium_ferric_citrate -.-> CHEBI_31604
    ing5_Methane_gas["Methane gas"]
    CultureMech_007848 --> ing5_Methane_gas
    ing6_Air["Air"]
    CultureMech_007848 --> ing6_Air
    ing7_KH2PO4["KH2PO4"]
    CultureMech_007848 --> ing7_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing7_KH2PO4 -.-> CHEBI_63036
    ing8_Na2HPO4_2H2O["Na2HPO4・2H2O"]
    CultureMech_007848 --> ing8_Na2HPO4_2H2O
    CHEBI_34683["CHEBI:34683"]
    ing8_Na2HPO4_2H2O -.-> CHEBI_34683
    ing9_Vitamin_B12["Vitamin B12"]
    CultureMech_007848 --> ing9_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing9_Vitamin_B12 -.-> CHEBI_176843
    ing10_Nicotinamide["Nicotinamide"]
    CultureMech_007848 --> ing10_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing10_Nicotinamide -.-> CHEBI_17154
    ing11_L_Ascorbic_acid["L-Ascorbic acid"]
    CultureMech_007848 --> ing11_L_Ascorbic_acid
    CHEBI_29073["CHEBI:29073"]
    ing11_L_Ascorbic_acid -.-> CHEBI_29073

Ingredients (12)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	101.0 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 2 duplicates: 1.0, 100.0]
2	MgSO4・7H2O 7H2O.Mg.O4S 246.473 g/mol	1 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3	CaCl2・2H2O Ca.2Cl.2H2O 147.014 g/mol	0.2 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
4	KNO3 K.NO3 101.102 g/mol	0.25 G_PER_L	CHEBI:63043 potassium nitrate	Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
5	Ammonium ferric citrate	4 G_PER_L	CHEBI:31604 ferric ammonium citrate	Role: Nitrogen source; Properties: Organic compound, Simple component, Defined component
6	Methane gas	variable VARIABLE		Properties: ガス
7	Air	variable VARIABLE		Properties: ガス
8	KH2PO4 H2O4P.K 136.084 g/mol	1.4 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
9	Na2HPO4・2H2O HO4P.2Na 141.958 g/mol	3.6 G_PER_L	CHEBI:34683 disodium hydrogenphosphate	Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
10	Vitamin B12	20 G_PER_L	CHEBI:176843 vitamin B12	Role: Vitamin; Properties: Simple component, Organic compound, Defined component
11	Nicotinamide C6H6N2O 122.127 g/mol	5 G_PER_L	CHEBI:17154 nicotinamide	Role: Growth factor; Properties: Organic compound, Simple component, Defined component
12	L-Ascorbic acid C6H8O6 176.124 g/mol	5 G_PER_L	CHEBI:29073 L-ascorbic acid	Role: Vitamin; Properties: Defined component, Simple component, Organic compound

Solutions

1 M HEPES solution (pH 7.0)

Dose: 2 G_PER_L

Role: Buffer; Properties: Defined component, Solution

FeCl2 solution (see Medium [M180])

Dose: 1 G_PER_L

Cross-reference to Medium M180

Trace element solution (see Medium [M180])

Dose: 1 G_PER_L

Cross-reference to Medium M180

Phosphate buffer solution (see below)

Dose: 2 G_PER_L

Vitamin solution (see below)

Dose: 10 G_PER_L

Trace vitamins (see Medium [M190])

Dose: 1 G_PER_L

Cross-reference to Medium M190

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:54.338840Z	togo-import	Imported from TOGO Medium	Source: JCM, ID: M1312
2026-01-28T19:48:34.460663Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: Methane gas; Added default concentration for ingredient: Air
2026-03-09T08:04:57.233408+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 6 solution(s) from ingredients
2026-03-13T03:09:28.812249Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 1 duplicate ingredient(s)
2026-03-13T06:19:51.938296Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:007848
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:38.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:10.379715+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Modified Nitrate Mineral Salts Medium-2" to "modified_nitrate_mineral_salts_medium_2" for consistent matching
2026-05-16T09:03:09.326790+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:40:32.282029+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:22.654083+00:00	mgso4-heptahydrate-term-fix-v1.0	Corrected MgSO4·7H2O primary term grounding to heptahydrate	Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:47.564971+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.