medium_for_treponema_pectinovorum

CultureMech:007908 bacterial COMPLEX SOLID_AGAR
Original name: Medium For Treponema Pectinovorum

Overview

External ontology
TOGO:M136 — Medium For Treponema Pectinovorum
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M136 Original source: JCM - JCM_M145 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=145

Composition

flowchart LR
    CultureMech_007908["medium_for_treponema_pectinovorum"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007908 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_007908 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_007908 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_K2HPO4["K2HPO4"]
    CultureMech_007908 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4__NH4_2SO4["(NH4)2SO4"]
    CultureMech_007908 --> ing4__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing4__NH4_2SO4 -.-> CHEBI_62946
    ing5_Pectin["Pectin"]
    CultureMech_007908 --> ing5_Pectin
    CHEBI_17309["CHEBI:17309"]
    ing5_Pectin -.-> CHEBI_17309
    ing6_Rumen_fluid["Rumen fluid"]
    CultureMech_007908 --> ing6_Rumen_fluid
    ing7_Bacto_agar__BD_Difco_["Bacto agar (BD-Difco)"]
    CultureMech_007908 --> ing7_Bacto_agar__BD_Difco_
    ing8_Heart_infusion_broth__BD_Difco_["Heart infusion broth (BD-Difco)"]
    CultureMech_007908 --> ing8_Heart_infusion_broth__BD_Difco_
    ing9_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
    CultureMech_007908 --> ing9_L__Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing9_L__Cysteine_HCl_H2O -.-> CHEBI_91248
    ing10_Polypeptone_peptone__BD_BBL_["Polypeptone peptone (BD-BBL)"]
    CultureMech_007908 --> ing10_Polypeptone_peptone__BD_BBL_
    ing11_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_007908 --> ing11_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing11_Carbon_dioxide_gas -.-> CHEBI_16526
    ing12_Nitrogen_gas["Nitrogen gas"]
    CultureMech_007908 --> ing12_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing12_Nitrogen_gas -.-> CHEBI_17997

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
500 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 Yeast extract 5 G_PER_L Role: Nutrient source; Properties: Undefined component
3 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
4 K2HPO4
HO4P.2K 174.174 g/mol
2 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
5 (NH4)2SO4
2H4N.O4S 132.141 g/mol
2 G_PER_L CHEBI:62946
ammonium sulfate
 Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component
6 Pectin 0.8 G_PER_L CHEBI:17309
pectin
 Role: Carbon source; Properties: Organic compound, Simple component, Defined component
7 Rumen fluid 500 G_PER_L Role: Nutrient source; Properties: Undefined component
8 Bacto agar (BD-Difco) 1 G_PER_L Role: Solidifying component
9 Heart infusion broth (BD-Difco) 5 G_PER_L Properties: Undefined component, Complex component
10 L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.68 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
 Properties: Organic compound, Simple component, Defined component
11 Polypeptone peptone (BD-BBL) 5 G_PER_L Properties: Undefined component
12 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
13 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス

Solutions

0.25% Resazurin solution

Dose: 4 G_PER_L

Role: pH dependent redox indicator; Properties: Defined component, Solution

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:54.425380Z togo-import Imported from TOGO Medium Source: JCM, ID: M136
2026-01-28T19:48:15.580147Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:04:25.733909+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:52.400059Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007908
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:58.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:10.857769+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Medium For Treponema Pectinovorum" to "medium_for_treponema_pectinovorum" for consistent matching
2026-05-16T09:03:09.750520+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:32.753335+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:47.718675+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:08.385773+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 009ea251b5bf0c76224d5889171ab6c5a43c462eddec70dbd1161f43a35eda22