Overview
- External ontology
- TOGO:M136 — Medium For Treponema Pectinovorum
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M136 Original source: JCM - JCM_M145 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=145
Composition
flowchart LR
CultureMech_007908["medium_for_treponema_pectinovorum"]
ing0_Distilled_water["Distilled water"]
CultureMech_007908 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_007908 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_007908 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_K2HPO4["K2HPO4"]
CultureMech_007908 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4__NH4_2SO4["(NH4)2SO4"]
CultureMech_007908 --> ing4__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing4__NH4_2SO4 -.-> CHEBI_62946
ing5_Pectin["Pectin"]
CultureMech_007908 --> ing5_Pectin
CHEBI_17309["CHEBI:17309"]
ing5_Pectin -.-> CHEBI_17309
ing6_Rumen_fluid["Rumen fluid"]
CultureMech_007908 --> ing6_Rumen_fluid
ing7_Bacto_agar__BD_Difco_["Bacto agar (BD-Difco)"]
CultureMech_007908 --> ing7_Bacto_agar__BD_Difco_
ing8_Heart_infusion_broth__BD_Difco_["Heart infusion broth (BD-Difco)"]
CultureMech_007908 --> ing8_Heart_infusion_broth__BD_Difco_
ing9_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_007908 --> ing9_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing9_L__Cysteine_HCl_H2O -.-> CHEBI_91248
ing10_Polypeptone_peptone__BD_BBL_["Polypeptone peptone (BD-BBL)"]
CultureMech_007908 --> ing10_Polypeptone_peptone__BD_BBL_
ing11_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007908 --> ing11_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing11_Carbon_dioxide_gas -.-> CHEBI_16526
ing12_Nitrogen_gas["Nitrogen gas"]
CultureMech_007908 --> ing12_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing12_Nitrogen_gas -.-> CHEBI_17997
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
500 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component |
| 6 | Pectin | 0.8 G_PER_L |
CHEBI:17309
pectin
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 7 | Rumen fluid | 500 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 8 | Bacto agar (BD-Difco) | 1 G_PER_L | Role: Solidifying component | |
| 9 | Heart infusion broth (BD-Difco) | 5 G_PER_L | Properties: Undefined component, Complex component | |
| 10 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.68 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Organic compound, Simple component, Defined component |
| 11 | Polypeptone peptone (BD-BBL) | 5 G_PER_L | Properties: Undefined component | |
| 12 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 13 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Solutions
0.25% Resazurin solution
Dose: 4 G_PER_L
Role: pH dependent redox indicator; Properties: Defined component, Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.425380Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M136 |
| 2026-01-28T19:48:15.580147Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:25.733909+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:52.400059Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007908 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.857769+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium For Treponema Pectinovorum" to "medium_for_treponema_pectinovorum" for consistent matching |
| 2026-05-16T09:03:09.750520+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:32.753335+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.718675+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |