natroniella_medium_for_anb_phb2

CultureMech:007919 bacterial COMPLEX LIQUID
Original name: Natroniella Medium For ANB-PHB2

Overview

External ontology
TOGO:M1385 — Natroniella Medium For ANB-PHB2
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1385 Original source: JCM - JCM_M1289 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1289

Composition

flowchart LR
    CultureMech_007919["natroniella_medium_for_anb_phb2"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007919 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_007919 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_K2HPO4["K2HPO4"]
    CultureMech_007919 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_NaHCO3["NaHCO3"]
    CultureMech_007919 --> ing3_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing3_NaHCO3 -.-> CHEBI_32139
    ing4_Na2CO3["Na2CO3"]
    CultureMech_007919 --> ing4_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing4_Na2CO3 -.-> CHEBI_29377
    ing5_HCl["HCl"]
    CultureMech_007919 --> ing5_HCl
    CHEBI_17883["CHEBI:17883"]
    ing5_HCl -.-> CHEBI_17883

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 NaCl
Cl.Na 58.443 g/mol
18 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
4 NaHCO3
CHO3.Na 84.006 g/mol
40 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
5 Na2CO3
CO3.2Na 105.988 g/mol
64 G_PER_L CHEBI:29377
sodium carbonate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
6 HCl
HCl 36.461 g/mol
variable VARIABLE CHEBI:17883
hydrogen chloride
 Role: Solvating media; Properties: Defined component, Inorganic compound, Solution

Solutions

1 N NH4Cl solution

Dose: 4 G_PER_L

Role: Nitrogen source; Properties: Inorganic compound, Solution, Defined component

1 M MgCl2 solution

Dose: 1 G_PER_L

Role: Mineral source

Trace element solution (see Medium [M1148])

Dose: 1 G_PER_L

Cross-reference to Medium M1148

Selenite--tungstate solution (see Medium [M431])

Dose: 1 G_PER_L

Cross-reference to Medium M431

Trace vitamins (see Medium [M190])

Dose: 1 G_PER_L

Cross-reference to Medium M190

1.0% Yeast extract solution

Dose: 2 G_PER_L

Role: Nutrient source; Properties: Undefined component

5% Na2S・9H2O solution

Dose: 2.5 G_PER_L

Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound

2.0 M Sodium crotonate solution*

Dose: 10 G_PER_L

Role: Carbon source; Properties: Solution

0.1% Sodium dithionite solution (in 1 M NaHCO3)*

Dose: 2 G_PER_L

Properties: Defined component, Inorganic compound, Solution

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:54.462774Z togo-import Imported from TOGO Medium Source: JCM, ID: M1385
2026-01-28T19:48:22.818790Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: HCl
2026-03-09T08:04:37.981264+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 9 solution(s) from ingredients
2026-03-13T06:19:52.478806Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007919
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:14.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:10.940196+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Natroniella Medium For ANB-PHB2" to "natroniella_medium_for_anb_phb2" for consistent matching
2026-05-16T09:03:09.839289+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:32.842781+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:47.741439+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:08.493391+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 16925f8bf8397a637b5eb0a702a26085e2cde4791bcc56728c758fc68cb4e522