Overview
- External ontology
- TOGO:M1385 — Natroniella Medium For ANB-PHB2
- Applications
-
- Microbial cultivation
- pH
- 9.6
- Notes
- TOGO M1385 imports JCM Medium 1289. MediaDive J1289 preserves the pH 9.6 base carbonate-phosphate recipe, the nine per-liter stock additions, and anaerobic distribution under an argon gas stream.
Composition
flowchart LR
CultureMech_007919["natroniella_medium_for_anb_phb2"]
ing0_Na2CO3["Na2CO3"]
CultureMech_007919 --> ing0_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing0_Na2CO3 -.-> CHEBI_29377
ing1_NaHCO3["NaHCO3"]
CultureMech_007919 --> ing1_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing1_NaHCO3 -.-> CHEBI_32139
ing2_NaCl["NaCl"]
CultureMech_007919 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_K2HPO4["K2HPO4"]
CultureMech_007919 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Distilled_water["Distilled water"]
CultureMech_007919 --> ing4_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing4_Distilled_water -.-> CHEBI_15377
ing5_HCl["HCl"]
CultureMech_007919 --> ing5_HCl
CHEBI_17883["CHEBI:17883"]
ing5_HCl -.-> CHEBI_17883
ing6_Ar["Ar"]
CultureMech_007919 --> ing6_Ar
CHEBI_49474["CHEBI:49474"]
ing6_Ar -.-> CHEBI_49474
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2CO3
CO3.2Na 105.988 g/mol
|
64.0 G_PER_L |
CHEBI:29377
sodium carbonate
|
TOGO M1385 / MediaDive J1289 lists 64.0 g/L Na2CO3. |
| 2 |
NaHCO3
CHO3.Na 84.006 g/mol
|
40.0 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
TOGO M1385 / MediaDive J1289 lists 40.0 g/L NaHCO3. |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
18.0 G_PER_L |
CHEBI:26710
sodium chloride
|
TOGO M1385 / MediaDive J1289 lists 18.0 g/L NaCl. |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.0 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
TOGO M1385 / MediaDive J1289 lists 1.0 g/L K2HPO4. |
| 5 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
MediaDive J1289 records the base salts as brought to 1.0 L with distilled water before pH adjustment and autoclaving. |
| 6 |
HCl
HCl 36.461 g/mol
|
variable VARIABLE |
CHEBI:17883
hydrogen chloride
|
MediaDive J1289 adjusts the base to pH 9.6 with 6N HCl. |
| 7 |
Ar
Ar 39.948 g/mol
|
variable VARIABLE |
CHEBI:49474
argon(0)
|
MediaDive J1289 distributes the completed medium into culture vessels under an argon gas stream. |
Preparation
- Add Na2CO3, NaHCO3, NaCl, and K2HPO4 to distilled water and bring the volume to 1.0 L.
- Adjust to pH 9.6 with 6N HCl.
- Autoclave the base medium.
- After cooling, aseptically add autoclaved and filter-sterilized stock solutions.
- Aseptically and anaerobically distribute the medium into culture vessels under an argon gas stream and seal with butyl rubber stoppers.
Solutions
1 N NH4Cl solution
Dose: 4.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NH4Cl | 1.0 MOLAR | 1 N NH4Cl solution is represented from the stock label as 1.0 M NH4Cl. |
MediaDive J1289 adds 4.0 ml/L 1 N NH4Cl solution.
1 M MgCl2 solution
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MgCl2 | 1.0 MOLAR | 1 M MgCl2 solution is represented from the stock label as 1.0 M MgCl2. |
MediaDive J1289 adds 1.0 ml/L 1 M MgCl2 solution.
Trace element solution
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| EDTA | 5.0 G_PER_L | TOGO M1385 / MediaDive J1289 lists 5.0 g/L EDTA. |
| FeSO4 x 7H2O | 2.0 G_PER_L | TOGO M1385 / MediaDive J1289 lists 2.0 g/L FeSO4 x 7H2O. |
| ZnSO4 x 7H2O | 0.1 G_PER_L | TOGO M1385 / MediaDive J1289 lists 0.1 g/L ZnSO4 x 7H2O. |
| MnCl2 x 4H2O | 0.03 G_PER_L | TOGO M1385 / MediaDive J1289 lists 0.03 g/L MnCl2 x 4H2O. |
| H3BO3 | 0.3 G_PER_L | TOGO M1385 / MediaDive J1289 lists 0.3 g/L H3BO3. |
| CoCl2 x 6H2O | 0.2 G_PER_L | TOGO M1385 / MediaDive J1289 lists 0.2 g/L CoCl2 x 6H2O. |
| CuCl2 x 2H2O | 0.01 G_PER_L | TOGO M1385 / MediaDive J1289 lists 0.01 g/L CuCl2 x 2H2O. |
| NiCl2 x 6H2O | 0.02 G_PER_L | TOGO M1385 / MediaDive J1289 lists 0.02 g/L NiCl2 x 6H2O. |
| Na2MoO4 x 2H2O | 0.03 G_PER_L | TOGO M1385 / MediaDive J1289 lists 0.03 g/L Na2MoO4 x 2H2O. |
| Distilled water | 1.0 L | TOGO M1385 / MediaDive J1289 lists 1.0 L Distilled water. |
MediaDive J1289 adds 1.0 ml/L Trace element solution and preserves the stock recipe as a 1.0 L solution.
Selenite-tungstate solution
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaOH | 0.4 G_PER_L | TOGO M1385 / MediaDive J1289 lists 0.4 g/L NaOH. |
| Na2SeO3 x 5H2O | 6.0 MG_PER_L | TOGO M1385 / MediaDive J1289 lists 6.0 mg/L Na2SeO3 x 5H2O. |
| Na2WO4 x 2H2O | 8.0 MG_PER_L | TOGO M1385 / MediaDive J1289 lists 8.0 mg/L Na2WO4 x 2H2O. |
| Distilled water | 1.0 L | TOGO M1385 / MediaDive J1289 lists 1.0 L Distilled water. |
MediaDive J1289 adds 1.0 ml/L filter-sterilized Selenite-tungstate solution.
Trace vitamins
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 2.0 MG_PER_L | TOGO M1385 / MediaDive J1289 lists 2.0 mg/L Biotin. |
| Folic acid | 2.0 MG_PER_L | TOGO M1385 / MediaDive J1289 lists 2.0 mg/L Folic acid. |
| Pyridoxine hydrochloride | 10.0 MG_PER_L | TOGO M1385 / MediaDive J1289 lists 10.0 mg/L Pyridoxine hydrochloride. |
| Thiamine HCl | 5.0 MG_PER_L | TOGO M1385 / MediaDive J1289 lists 5.0 mg/L Thiamine HCl. |
| Riboflavin | 5.0 MG_PER_L | TOGO M1385 / MediaDive J1289 lists 5.0 mg/L Riboflavin. |
| Nicotinic acid | 5.0 MG_PER_L | TOGO M1385 / MediaDive J1289 lists 5.0 mg/L Nicotinic acid. |
| Calcium pantothenate | 5.0 MG_PER_L | TOGO M1385 / MediaDive J1289 lists 5.0 mg/L Calcium pantothenate. |
| Vitamin B12 | 0.1 MG_PER_L | TOGO M1385 / MediaDive J1289 lists 0.1 mg/L Vitamin B12. |
| p-Aminobenzoic acid | 5.0 MG_PER_L | TOGO M1385 / MediaDive J1289 lists 5.0 mg/L p-Aminobenzoic acid. |
| Lipoic acid | 5.0 MG_PER_L | TOGO M1385 / MediaDive J1289 lists 5.0 mg/L Lipoic acid. |
| Distilled water | 1.0 L | TOGO M1385 / MediaDive J1289 lists 1.0 L Distilled water. |
MediaDive J1289 adds 1.0 ml/L filter-sterilized Trace vitamins.
1.0% Yeast extract solution
Dose: 2.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Yeast extract | 1.0 PERCENT_W_V | 1.0% Yeast extract solution is represented from the stock label as 1.0 % w/v Yeast extract. |
MediaDive J1289 adds 2.0 ml/L 1.0% Yeast extract solution.
5% Na2S x 9H2O solution
Dose: 2.5 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2S x 9H2O | 5.0 PERCENT_W_V | 5% Na2S x 9H2O solution is represented from the stock label as 5.0 % w/v Na2S x 9H2O. |
MediaDive J1289 adds 2.5 ml/L 5% Na2S x 9H2O solution.
2.0 M Sodium crotonate solution
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Sodium crotonate | 2.0 MOLAR | 2.0 M Sodium crotonate solution is represented from the stock label as 2.0 M Sodium crotonate. |
MediaDive J1289 adds 10.0 ml/L filter-sterilized 2.0 M Sodium crotonate solution.
0.1% Sodium dithionite solution (in 1 M NaHCO3)
Dose: 2.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Sodium dithionite | 0.1 PERCENT_W_V | TOGO M1385 / MediaDive J1289 lists 0.1 % w/v Sodium dithionite. |
| NaHCO3 | 1.0 MOLAR | TOGO M1385 / MediaDive J1289 lists 1.0 M NaHCO3. |
MediaDive J1289 adds 2.0 ml/L filter-sterilized 0.1% Sodium dithionite solution in 1 M NaHCO3.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.462774Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1385 |
| 2026-01-28T19:48:22.818790Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: HCl |
| 2026-03-09T08:04:37.981264+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 9 solution(s) from ingredients |
| 2026-03-13T06:19:52.478806Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007919 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:14.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.940196+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Natroniella Medium For ANB-PHB2" to "natroniella_medium_for_anb_phb2" for consistent matching |
| 2026-05-16T09:03:09.839289+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:32.842781+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.741439+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m1385_score15.py | RESOLVED_TOGO_M1385_SCORE15 | TOGO M1385 imports JCM Medium 1289. MediaDive J1289 preserves the pH 9.6 base carbonate-phosphate recipe, the nine per-liter stock additions, and anaerobic distribution under an argon gas stream. Expanded the empty TOGO stock wrappers with the nested stock compositions and corrected stock additions from g/L artifacts to ml/L volumes. |