Overview
- External ontology
- TOGO:M1485 — Modified Hayflick Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1485 Original source: NBRC - NBRC_M267 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=267
Composition
flowchart LR
CultureMech_008029["modified_hayflick_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008029 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Bacto_Agar__Difco__if_needed_["Bacto Agar (Difco, if needed)"]
CultureMech_008029 --> ing1_Bacto_Agar__Difco__if_needed_
ing2_Bacto_Heart_Infusion_Broth__Difco_["Bacto Heart Infusion Broth (Difco)"]
CultureMech_008029 --> ing2_Bacto_Heart_Infusion_Broth__Difco_
ing3_calf_thymus_DNA__Sigma__Type_I_["calf thymus DNA (Sigma, Type I)"]
CultureMech_008029 --> ing3_calf_thymus_DNA__Sigma__Type_I_
ing4_thallium_acetate["thallium acetate"]
CultureMech_008029 --> ing4_thallium_acetate
ing5_horse_serum__heat_inactivated__56__for_30_minutes_["horse serum (heat inactivated, 56℃ for 30 minutes)"]
CultureMech_008029 --> ing5_horse_serum__heat_inactivated__56__for_30_minutes_
ing6_Penicillin_G["Penicillin G"]
CultureMech_008029 --> ing6_Penicillin_G
ing7_dried_baker_s_yeast["dried baker's yeast"]
CultureMech_008029 --> ing7_dried_baker_s_yeast
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
701.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 700.0, 1.0] |
| 2 | Bacto Agar (Difco, if needed) | 15 G_PER_L | Role: Solidifying component | |
| 3 | Bacto Heart Infusion Broth (Difco) | 25 G_PER_L | Properties: Undefined component, Complex component | |
| 4 | calf thymus DNA (Sigma, Type I) | 12 G_PER_L | Role: Growth factor | |
| 5 | thallium acetate | 10 G_PER_L | Properties: Defined component, Simple component | |
| 6 | horse serum (heat inactivated, 56℃ for 30 minutes) | 200 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 7 | Penicillin G | 25 G_PER_L | Role: Antimicrobial agent; Properties: Simple component, Defined component | |
| 8 | dried baker's yeast | 250 G_PER_L | Role: Nutrient source; Properties: Undefined component |
Solutions
fresh yeast extract (25% solution)
Dose: 100 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_hayflick_medium (CultureMech:008029)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.743606Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1485 |
| 2026-01-28T19:48:10.281449Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:16.803955+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:08:44.253980Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:53.100723Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008029 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:11.604932+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified Hayflick Medium" to "modified_hayflick_medium" for consistent matching |
| 2026-05-16T09:04:25.417028+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:36.697269+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:48.369630+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 853a5703614af5a118bdabac12052e9646b6e4d58ad13f4260728193d9eadf64 |