modified_hayflick_medium

CultureMech:008029 bacterial COMPLEX SOLID_AGAR
Original name: Modified Hayflick Medium

Overview

External ontology
TOGO:M1485 — Modified Hayflick Medium
Applications
  • Microbial cultivation
pH
7.8
Notes
TOGO M1485 imports NBRC Medium 267 as Modified Hayflick Medium. NBRC 267 lists 25 g Bacto Heart Infusion Broth, 700 ml distilled water, 15 g Bacto Agar if needed, pH 7.8, autoclaving at 121 C for 20 min, and aseptic addition of 200 ml heat-inactivated horse serum, 100 ml fresh yeast extract, 12 ml calf thymus DNA, 10 ml thallium acetate, and 25 ml Penicillin G.

Composition

flowchart LR
    CultureMech_008029["modified_hayflick_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008029 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Bacto_Agar__Difco__if_needed_["Bacto Agar (Difco, if needed)"]
    CultureMech_008029 --> ing1_Bacto_Agar__Difco__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing1_Bacto_Agar__Difco__if_needed_ -.-> CHEBI_2509
    ing2_Bacto_Heart_Infusion_Broth__Difco_["Bacto Heart Infusion Broth (Difco)"]
    CultureMech_008029 --> ing2_Bacto_Heart_Infusion_Broth__Difco_
    ing3_calf_thymus_DNA__Sigma__Type_I_["calf thymus DNA (Sigma, Type I)"]
    CultureMech_008029 --> ing3_calf_thymus_DNA__Sigma__Type_I_
    ing4_thallium_acetate["thallium acetate"]
    CultureMech_008029 --> ing4_thallium_acetate
    CHEBI_75192["CHEBI:75192"]
    ing4_thallium_acetate -.-> CHEBI_75192
    ing5_horse_serum__heat_inactivated__56__for_30_minutes_["horse serum (heat inactivated, 56℃ for 30 minutes)"]
    CultureMech_008029 --> ing5_horse_serum__heat_inactivated__56__for_30_minutes_
    ing6_Penicillin_G["Penicillin G"]
    CultureMech_008029 --> ing6_Penicillin_G
    CHEBI_51765["CHEBI:51765"]
    ing6_Penicillin_G -.-> CHEBI_51765

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
700.0 ML_PER_L CHEBI:15377
water
 TOGO M1485 / NBRC Medium 267 lists 700 ml/L Distilled water.
2 Bacto Agar (Difco, if needed) 15.0 G_PER_L CHEBI:2509
agar
 TOGO M1485 / NBRC Medium 267 lists 15 g/L Bacto Agar from Difco if a solid medium is needed.
3 Bacto Heart Infusion Broth (Difco) 25.0 G_PER_L TOGO M1485 / NBRC Medium 267 lists 25 g/L Bacto Heart Infusion Broth from Difco; this catalog broth is retained as an opaque component.
4 calf thymus DNA (Sigma, Type I) 12.0 ML_PER_L TOGO M1485 / NBRC Medium 267 lists 12 ml/L calf thymus DNA from Sigma Type I as a 0.2% w/v stock; this source-qualified DNA product remains intentionally unmapped.
5 thallium acetate
C2H3O2.Tl 263.427 g/mol
10.0 ML_PER_L CHEBI:75192
Thallium(I) acetate
 TOGO M1485 / NBRC Medium 267 lists 10 ml/L thallium acetate as a 1% w/v stock.
6 horse serum (heat inactivated, 56℃ for 30 minutes) 200.0 ML_PER_L MICRO:0001235 (source link unavailable)
Horse serum
 TOGO M1485 / NBRC Medium 267 lists 200 ml/L horse serum heat inactivated at 56 C for 30 minutes.
7 Penicillin G
C16H17N2O4S.Na 356.379 g/mol
25.0 ML_PER_L CHEBI:51765
benzylpenicillin sodium
 TOGO M1485 / NBRC Medium 267 lists 25 ml/L Penicillin G as a 20,000 IU/ml stock.

Preparation

  1. Combine 25 g Bacto Heart Infusion Broth and 700 ml distilled water; add 15 g Bacto Agar if a solid medium is needed.
  2. Adjust the base to pH 7.8.
  3. Autoclave at 121 C for 20 min.
  4. Aseptically add heat-inactivated horse serum, fresh yeast extract, calf thymus DNA, thallium acetate, and Penicillin G.

Solutions

fresh yeast extract (25% solution)

Dose: 100.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Distilled water 1.0 L TOGO M1485 / NBRC Medium 267 prepares the 25% fresh yeast extract with 1 L Distilled water.
dried baker's yeast 250.0 G_PER_L TOGO M1485 / NBRC Medium 267 boils 250 g dried baker's yeast per liter to prepare 25% fresh yeast extract.
TOGO M1485 / NBRC Medium 267 adds 100 ml/L fresh yeast extract prepared as a 25% solution.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:57.743606Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1485
2026-01-28T19:48:10.281449Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:16.803955+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T03:08:44.253980Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:53.100723Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008029
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:46.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:11.604932+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified Hayflick Medium" to "modified_hayflick_medium" for consistent matching
2026-05-16T09:04:25.417028+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:36.697269+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-20T19:02:55.977624+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-09-12T00:00:00-07:00 repair_togo_m1485_modified_hayflick_score15.py RESOLVED_TOGO_M1485_SCORE15 TOGO M1485 imports NBRC Medium 267 as Modified Hayflick Medium. NBRC 267 lists 25 g Bacto Heart Infusion Broth, 700 ml distilled water, 15 g Bacto Agar if needed, pH 7.8, autoclaving at 121 C for 20 min, and aseptic addition of 200 ml heat-inactivated horse serum, 100 ml fresh yeast extract, 12 ml calf thymus DNA, 10 ml thallium acetate, and 25 ml Penicillin G. Split the merged water row back into the 700 ml/L base water and the 1 L fresh-yeast-extract stock water, restored the fresh yeast extract stock composition, corrected stock addition volumes, added pH 7.8, and grounded machine-usable ingredients.