Overview
- External ontology
- TOGO:M154 — PPLO-Glucose Broth
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M154 Original source: JCM - JCM_M163 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=163
Composition
flowchart LR
CultureMech_008098["pplo_glucose_broth"]
ing0_Distilled_water["Distilled water"]
CultureMech_008098 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Sterile_horse_serum["Sterile horse serum"]
CultureMech_008098 --> ing1_Sterile_horse_serum
ing2_Glucose["Glucose"]
CultureMech_008098 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_PPLO_broth__BD_Difco_["PPLO broth (BD-Difco)"]
CultureMech_008098 --> ing3_PPLO_broth__BD_Difco_
ing4_Penicillin["Penicillin"]
CultureMech_008098 --> ing4_Penicillin
CHEBI_17334["CHEBI:17334"]
ing4_Penicillin -.-> CHEBI_17334
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
700 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Sterile horse serum | 200 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Simple component, Defined component, Organic compound |
| 4 | PPLO broth (BD-Difco) | 21 G_PER_L | Properties: Undefined component, Complex component | |
| 5 |
Penicillin
C9H11N2O4SR 243.26 g/mol
|
variable VARIABLE |
CHEBI:17334
penicillin
|
Role: Antimicrobial agent; Properties: Defined component, Organic compound, Simple component |
Solutions
0.4% Phenol red solution
Dose: 5 G_PER_L
Role: pH indicator; Properties: Defined component, Organic compound, Solution
25% Solution of fresh baker's yeast extract (Gibco 360-8180)
Dose: 100 G_PER_L
Role: Nutrient source; Properties: Undefined component
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M154_PPLO-Glucose_Broth (CultureMech:008098)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.525986Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M154 |
| 2026-01-28T19:48:37.652792Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Penicillin |
| 2026-03-09T08:05:02.856629+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:19:53.480695Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008098 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:12.016002+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PPLO-Glucose Broth" to "pplo_glucose_broth" for consistent matching |
| 2026-05-16T09:03:10.163867+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:33.230581+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:08.928529+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint ddd2380ade8b7bc006c9117dc1ef97b3baca21519c5078454d43212dbdd2bacb |