pplo_glucose_broth

CultureMech:008098 bacterial COMPLEX LIQUID
Original name: PPLO-Glucose Broth

Overview

External ontology
TOGO:M154 — PPLO-Glucose Broth
Applications
  • Microbial cultivation
pH
7.6
Notes
TOGO M154 imports JCM Medium 163 as PPLO-Glucose Broth. TOGO lists 5 ml 0.4% Phenol red solution, 100 ml 25% Solution of fresh baker's yeast extract (Gibco 360-8180), 700 ml distilled water, 200 ml sterile horse serum, 5 g glucose, 21 g PPLO broth from BD-Difco, and Penicillin; the pH is adjusted to 7.6. MediaDive J163 mirrors the retired JCM recipe and records the Penicillin amount as 1,000,000 U. The current JCM URL for Medium 163 no longer exposes the recipe.

Composition

flowchart LR
    CultureMech_008098["pplo_glucose_broth"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008098 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Sterile_horse_serum["Sterile horse serum"]
    CultureMech_008098 --> ing1_Sterile_horse_serum
    ing2_Glucose["Glucose"]
    CultureMech_008098 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_PPLO_broth__BD_Difco_["PPLO broth (BD-Difco)"]
    CultureMech_008098 --> ing3_PPLO_broth__BD_Difco_
    ing4_Penicillin["Penicillin"]
    CultureMech_008098 --> ing4_Penicillin
    CHEBI_17334["CHEBI:17334"]
    ing4_Penicillin -.-> CHEBI_17334

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
700.0 ML_PER_L CHEBI:15377
water
 TOGO M154 / JCM Medium 163 lists 700 ml distilled water.
2 Sterile horse serum 200.0 ML_PER_L TOGO M154 / JCM Medium 163 lists 200 ml sterile horse serum; this serum is retained as an opaque complex component.
3 Glucose
C6H12O6 180.156 g/mol
5.0 G_PER_L CHEBI:17234
glucose
 TOGO M154 / JCM Medium 163 lists 5.0 g/L Glucose.
4 PPLO broth (BD-Difco) 21.0 G_PER_L TOGO M154 / JCM Medium 163 lists 21 g PPLO broth from BD-Difco; the catalog broth is not reducible to one ChEBI molecule.
5 Penicillin
C9H11N2O4SR 243.26 g/mol
variable VARIABLE CHEBI:17334
penicillin
 TOGO M154 / JCM Medium 163 lists Penicillin without a mass or volume. MediaDive J163 reports the source JCM recipe used 1,000,000 U, which is retained as a variable concentration because the schema has no unit for activity units.

Preparation

  1. Adjust pH to 7.6.

Solutions

0.4% Phenol red solution

Dose: 5.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Phenol red 0.4 PERCENT_W_V TOGO M154 / JCM Medium 163 identifies the pH indicator stock as 0.4% Phenol red solution.
TOGO M154 / JCM Medium 163 lists 5 ml of 0.4% Phenol red solution.

25% Solution of fresh baker's yeast extract (Gibco 360-8180)

Dose: 100.0 ML_PER_L

TOGO M154 / JCM Medium 163 lists 100 ml of 25% Solution of fresh baker's yeast extract (Gibco 360-8180); this commercial yeast extract solution is retained as an opaque complex component.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.525986Z togo-import Imported from TOGO Medium Source: JCM, ID: M154
2026-01-28T19:48:37.652792Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Penicillin
2026-03-09T08:05:02.856629+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T06:19:53.480695Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008098
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:30:45.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:12.016002+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PPLO-Glucose Broth" to "pplo_glucose_broth" for consistent matching
2026-05-16T09:03:10.163867+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:33.230581+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m154_score15.py RESOLVED_TOGO_M154_SCORE15 TOGO M154 imports JCM Medium 163 as PPLO-Glucose Broth. TOGO lists 5 ml 0.4% Phenol red solution, 100 ml 25% Solution of fresh baker's yeast extract (Gibco 360-8180), 700 ml distilled water, 200 ml sterile horse serum, 5 g glucose, 21 g PPLO broth from BD-Difco, and Penicillin; the pH is adjusted to 7.6. MediaDive J163 mirrors the retired JCM recipe and records the Penicillin amount as 1,000,000 U. The current JCM URL for Medium 163 no longer exposes the recipe. Corrected ml import artifacts, added the source pH, kept PPLO broth and serum unmapped, and retained Penicillin as a variable-concentration antibiotic.