Overview
- External ontology
- TOGO:M172 — Medium 10 Broth
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M172 Original source: JCM - JCM_M179 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=179
Composition
flowchart LR
CultureMech_008293["medium_10_broth"]
ing0_Distilled_water["Distilled water"]
CultureMech_008293 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Cellobiose["Cellobiose"]
CultureMech_008293 --> ing1_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing1_Cellobiose -.-> CHEBI_17057
ing2_Glucose["Glucose"]
CultureMech_008293 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Soluble_starch["Soluble starch"]
CultureMech_008293 --> ing3_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing3_Soluble_starch -.-> CHEBI_28017
ing4_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_008293 --> ing4_Yeast_extract__BD_Difco_
ing5_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
CultureMech_008293 --> ing5_Trypticase_peptone__BD_BBL_
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
850 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
Cellobiose
C12H22O11 342.297 g/mol
|
0.5 G_PER_L |
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 4 | Soluble starch | 0.5 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 5 | Yeast extract (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 6 | Trypticase peptone (BD-BBL) | 2 G_PER_L | Properties: Undefined component |
Solutions
0.1% Resazurin solution
Dose: 0.5 G_PER_L
Role: pH dependent redox indicator; Properties: Solution, Defined component
0.2% Hemin solution
Dose: 0.5 G_PER_L
Role: Growth factor; Properties: Solution, Organic compound, Defined component
4% Na2SO3 solution
Dose: 100 G_PER_L
Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution
25% L--Ascorbic acid solution
Dose: 2 G_PER_L
Role: Vitamin; Properties: Organic compound, Solution, Defined component
5% L--Cysteine・HCl・H2O solution
Dose: 10 G_PER_L
Properties: Defined component, Organic compound, Solution
VFA solution (see Medium [M124])
Dose: 3.1 G_PER_L
Cross-reference to Medium M124
Salt solution No. 1 (see Medium [M124])
Dose: 37.5 G_PER_L
Cross-reference to Medium M124
Salt solution No. 2 (see Medium [M124])
Dose: 37.5 G_PER_L
Cross-reference to Medium M124
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M172_Medium_10_Broth (CultureMech:008293)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.552580Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M172 |
| 2026-01-28T19:48:11.462946Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:18.790736+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 8 solution(s) from ingredients |
| 2026-03-13T06:19:55.399198Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008293 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:13.998749+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium 10 Broth" to "medium_10_broth" for consistent matching |
| 2026-05-16T09:03:11.042838+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:34.157481+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:09.453682+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 5591ab3ec47fd69f5407bcc3a001c34015f03e4d122d6903cb228be45709b2cb |