medium_10_broth

CultureMech:008293 bacterial COMPLEX LIQUID
Original name: Medium 10 Broth

Overview

External ontology
TOGO:M172 — Medium 10 Broth
Applications
  • Microbial cultivation
pH range
{'min': 6.7, 'max': 6.8}
Notes
TOGO M172 preserves JCM Medium 179 as Medium 10 Broth. TOGO M172 records 850 ml distilled water, 0.5 g each of cellobiose, glucose, soluble starch, and Yeast extract from BD-Difco, 2 g Trypticase peptone from BD-BBL, 0.5 ml 0.1% Resazurin solution, 0.5 ml 0.2% Hemin solution, 100 ml 4% Na2SO3 solution, 2 ml 25% L-Ascorbic acid solution, 10 ml 5% L-Cysteine HCl H2O solution, and VFA and salt stocks from Medium 10 / TOGO M124, with pH adjusted to 6.7-6.8. It omits agar and Toray silicone from the JCM Medium 133 Medium 10 formulation. The current JCM Medium 179 URL no longer exposes the recipe.

Composition

flowchart LR
    CultureMech_008293["medium_10_broth"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008293 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Cellobiose["Cellobiose"]
    CultureMech_008293 --> ing1_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing1_Cellobiose -.-> CHEBI_17057
    ing2_Glucose["Glucose"]
    CultureMech_008293 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Soluble_starch["Soluble starch"]
    CultureMech_008293 --> ing3_Soluble_starch
    CHEBI_28017["CHEBI:28017"]
    ing3_Soluble_starch -.-> CHEBI_28017
    ing4_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_008293 --> ing4_Yeast_extract__BD_Difco_
    ing5_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
    CultureMech_008293 --> ing5_Trypticase_peptone__BD_BBL_

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
850.0 ML_PER_L CHEBI:15377
water
 TOGO M172 / JCM Medium 179 lists 850 ml distilled water.
2 Cellobiose
C12H22O11 342.297 g/mol
0.5 G_PER_L CHEBI:17057
cellobiose
 TOGO M172 / JCM Medium 179 lists 0.5 g/L Cellobiose.
3 Glucose
C6H12O6 180.156 g/mol
0.5 G_PER_L CHEBI:17234
glucose
 TOGO M172 / JCM Medium 179 lists 0.5 g/L Glucose.
4 Soluble starch 0.5 G_PER_L CHEBI:28017
starch
 TOGO M172 / JCM Medium 179 lists 0.5 g/L Soluble starch.
5 Yeast extract (BD-Difco) 0.5 G_PER_L FOODON:03315426
yeast extract
 TOGO M172 / JCM Medium 179 lists 0.5 g/L Yeast extract from BD-Difco.
6 Trypticase peptone (BD-BBL) 2.0 G_PER_L MICRO:0000175 (source link unavailable)
Trypticase peptone
 TOGO M172 / JCM Medium 179 lists 2 g/L Trypticase peptone from BD-BBL.

Preparation

  1. Adjust pH to 6.7-6.8.
  2. Use Medium 10 from JCM Medium 133 with 0.5 ml/L final 0.2% hemin solution, and omit agar and Toray silicone solution.

Solutions

0.1% Resazurin solution

Dose: 0.5 ML_PER_L

Stock composition
IngredientConcentrationNotes
Resazurin 0.1 PERCENT_W_V TOGO M172 / JCM Medium 179 identifies the redox indicator stock as 0.1% Resazurin.
TOGO M172 / JCM Medium 179 adds 0.5 ml/L 0.1% Resazurin solution.

0.2% Hemin solution

Dose: 0.5 ML_PER_L

Stock composition
IngredientConcentrationNotes
Hemin 0.2 PERCENT_W_V TOGO M172 / JCM Medium 179 identifies the growth-factor stock as 0.2% Hemin.
TOGO M172 / JCM Medium 179 adds 0.5 ml/L 0.2% Hemin solution.

4% Na2SO3 solution

Dose: 100.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Na2SO3 4.0 PERCENT_W_V TOGO M172 / JCM Medium 179 identifies the reducing stock as 4% Na2SO3.
TOGO M172 / JCM Medium 179 adds 100.0 ml/L 4% Na2SO3 solution.

25% L--Ascorbic acid solution

Dose: 2.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
L-Ascorbic acid 25.0 PERCENT_W_V TOGO M172 / JCM Medium 179 identifies the vitamin stock as 25% L-Ascorbic acid.
TOGO M172 / JCM Medium 179 adds 2.0 ml/L 25% L--Ascorbic acid solution.

5% L--Cysteine・HCl・H2O solution

Dose: 10.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
L-Cysteine HCl H2O 5.0 PERCENT_W_V TOGO M172 / JCM Medium 179 identifies the cysteine stock as 5% L-Cysteine HCl H2O.
TOGO M172 / JCM Medium 179 adds 10.0 ml/L 5% L--Cysteine・HCl・H2O solution.

VFA solution (see Medium [M124])

Dose: 3.1 ML_PER_L

TOGO M172 / JCM Medium 179 adds 3.1 ml/L VFA solution from Medium 10; TOGO M124 / JCM Medium 133 defines the source stock.

Salt solution No. 1 (see Medium [M124])

Dose: 37.5 ML_PER_L

TOGO M172 / JCM Medium 179 adds 37.5 ml/L Salt solution No. 1 from Medium 10; TOGO M124 / JCM Medium 133 defines the source stock.

Salt solution No. 2 (see Medium [M124])

Dose: 37.5 ML_PER_L

TOGO M172 / JCM Medium 179 adds 37.5 ml/L Salt solution No. 2 from Medium 10; TOGO M124 / JCM Medium 133 defines the source stock.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.552580Z togo-import Imported from TOGO Medium Source: JCM, ID: M172
2026-01-28T19:48:11.462946Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:18.790736+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 8 solution(s) from ingredients
2026-03-13T06:19:55.399198Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008293
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:49.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:13.998749+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Medium 10 Broth" to "medium_10_broth" for consistent matching
2026-05-16T09:03:11.042838+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:34.157481+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m172_score15.py RESOLVED_TOGO_M172_SCORE15 TOGO M172 preserves JCM Medium 179 as Medium 10 Broth. TOGO M172 records 850 ml distilled water, 0.5 g each of cellobiose, glucose, soluble starch, and Yeast extract from BD-Difco, 2 g Trypticase peptone from BD-BBL, 0.5 ml 0.1% Resazurin solution, 0.5 ml 0.2% Hemin solution, 100 ml 4% Na2SO3 solution, 2 ml 25% L-Ascorbic acid solution, 10 ml 5% L-Cysteine HCl H2O solution, and VFA and salt stocks from Medium 10 / TOGO M124, with pH adjusted to 6.7-6.8. It omits agar and Toray silicone from the JCM Medium 133 Medium 10 formulation. The current JCM Medium 179 URL no longer exposes the recipe. Corrected imported ml additions, added the TOGO pH range and Medium 10 note, represented simple concentration stocks, and marked the source formula as curated.
2026-09-12T00:00:00-07:00 repair_jcm_medium_10_duplicates_score15.py LINKED_JCM_MEDIUM_10_SOURCE_DUPLICATE Added or refreshed the reciprocal SOURCE_DUPLICATE link for bacterial/medium_10_broth.yaml.